Polymeric integration of structure-switching aptamers on transistors for histamine sensing

Shkodra, Bajramshahe; Petrelli, Mattia; Yang, Kiyull; Tagliaferri, Anna; Lugli, Paolo; Petti, Luisa; Nakatsuka, Nako

doi:10.1039/d3fd00123g

Cited by 8 publications

(3 citation statements)

References 61 publications

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“…Structure-switching aptamers provide a solution to overcome the limitations of λ D while amplifying small-molecule binding effects. 31 − 33 These aptamers undergo significant conformational rearrangements upon target capture, and the signal is amplified by the movement of counterions associated with the negatively charged oligonucleotide backbone. These aptamers facilitate signal transduction in close proximity to the sensing surface through target-induced structural rearrangements that alters the charge density within the sensitive region ( Figure 2 b).…”

Section: Aptamers As Molecular Recognition Elementsmentioning

confidence: 99%

Aptamer Renaissance for Neurochemical Biosensing

Stuber,

Nakatsuka

2024

ACS Nano

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Unraveling the complexities of brain function, which is crucial for advancing human health, remains a grand challenge. This endeavor demands precise monitoring of small molecules such as neurotransmitters, the chemical messengers in the brain. In this Perspective, we explore the potential of aptamers, selective synthetic bioreceptors integrated into electronic affinity platforms to address limitations in neurochemical biosensing. We emphasize the importance of characterizing aptamer thermodynamics and target binding to realize functional biosensors in biological systems. We focus on two label-free affinity platforms spanning the micro-to nanoscale: field-effect transistors and nanopores. Integration of wellcharacterized structure-switching aptamers overcame nonspecific binding, a challenge that has hindered the translation of biosensors from the lab to the clinic. In a transformative era driven by neuroscience breakthroughs, technological innovations, and multidisciplinary collaborations, an aptamer renaissance holds the potential to bridge technological gaps and reshape the landscape of diagnostics and neuroscience.

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Section: Aptamers As Molecular Recognition Elementsmentioning

confidence: 99%

Aptamer Renaissance for Neurochemical Biosensing

Stuber,

Nakatsuka

2024

ACS Nano

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“…32 Carbon nanotubes (CNTs) are an interesting type of ptype semiconductor material, because of their good electrical properties, their small size (nanometric), their high surface ratio, and their quite simple processability. 33 EG-FETs have also been used for the detection of environmental contaminants, for example pesticides. 31,34 However, to the best of our knowledge, this transduction platform has not yet been used for the detection of NPs.…”

Section: ■ Introductionmentioning

confidence: 99%

“…In p-type EG-FETs, when a negative voltage is applied between the source and the gate and between the source and the drain, the ions of the electrolyte rearrange themselves to form two electric double layers (EDLs) at the semiconductor-liquid interface and at the gate-liquid interface . Carbon nanotubes (CNTs) are an interesting type of p-type semiconductor material, because of their good electrical properties, their small size (nanometric), their high surface ratio, and their quite simple processability . EG-FETs have also been used for the detection of environmental contaminants, for example pesticides. , However, to the best of our knowledge, this transduction platform has not yet been used for the detection of NPs.…”

Section: Introductionmentioning

confidence: 99%

Electrolyte-Gated Carbon Nanotube Field-Effect Transistor-Based Sensors for Nanoplastics Detection in Seawater: A Study of the Interaction between Nanoplastics and Carbon Nanotubes

Elli,

Ciocca,

Shkodra

et al. 2024

ACS Appl. Mater. Interfaces

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Plastics accumulating in the environment are nowadays of great concern for aquatic systems and for the living organisms populating them. In this context, nanoplastics (NPs) are considered the major and most dangerous contaminants because of their small size and active surface, which allow them to interact with a variety of other molecules. Current methods used for the detection of NPs rely on bulky and expensive techniques such as spectroscopy. Here we propose, for the first time, a novel, fast, and easy-to-use sensor based on an electrolyte-gated field-effect transistor (EG-FET) with a carbon nanotube (CNT) semiconductor (EG-CNTFET) for the detection of NPs in aquatic environments, using polystyrene NPs (PS-NPs) as a model material. In particular, as a working mechanism for the EG-CNTFETs we exploited the ability of CNTs and PS to form noncovalent interactions. Indeed, in our EG-CNTFET devices, the interaction between NPs and CNTs caused a change in the electric double layers. A linear increase in the corrected on current (*I ON ) of the EG-CNTFETs, with a sensitivity of 9.68 μA/(1 mg/mL) and a linear range of detection from 0.025 to 0.25 mg/mL were observed. A π−π interaction was hypothesized to take place between the two materials, as indicated by an X-ray photoelectron spectroscopy analysis. Using artificial seawater as an electrolyte, to mimic a real-case scenario, a linear increase in *I ON was also observed, with a sensitivity of 6.19 μA/(1 mg/mL), proving the possibility to use the developed sensor in more complex solutions, as well as in low concentrations. This study offers a starting point for future exploitation of electrochemical sensors for NP detection and identification.

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Granular Biomaterials as Bioactive Sponges for the Sequestration and Release of Signaling Molecules

Emiroglu,

Singh,

Marco‐Dufort

et al. 2024

Adv Healthcare Materials

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A major challenge for the regeneration of chronic wounds is an underlying dysregulation of signaling molecules, including inflammatory cytokines and growth factors. To address this, we propose to use granular biomaterials composed of jammed microgels, to enable the rapid uptake and delivery of biomolecules, and provide a strategy to locally sequester and release biomolecules. Sequestration assays on model biomolecules of different sizes demonstrated that granular hydrogels exhibit faster transport than comparable bulk hydrogels due to enhanced surface area and decreased diffusion lengths. To demonstrate the potential of modular granular hydrogels to modulate local biomolecule concentrations, we engineered microgel scaffolds that can simultaneously sequester excess pro‐inflammatory factors and release pro‐healing factors. To target specific biomolecules, microgels were functionalized with affinity ligands that bind either to interleukin 6 (IL‐6) or to vascular endothelial growth factor A (VEGF‐A). Finally, disparate microgels were combined into a single granular biomaterial for simultaneous sequestration of IL‐6 and release of VEGF‐A. Overall, we demonstrate the potential of modular granular hydrogels to locally tailor the relative concentrations of pro‐ and anti‐inflammatory factors.This article is protected by copyright. All rights reserved

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Polymeric integration of structure-switching aptamers on transistors for histamine sensing

Abstract: Aptamers that undergo large conformational rearrangements at the surface of electrolyte-gated field-effect transistor (EG-FETs)-based biosensors can overcome the Debye length limitation in physiological high ionic strength environments. For the sensitive...

Cited by 8 publications

References 61 publications

Aptamer Renaissance for Neurochemical Biosensing

Aptamer Renaissance for Neurochemical Biosensing

Electrolyte-Gated Carbon Nanotube Field-Effect Transistor-Based Sensors for Nanoplastics Detection in Seawater: A Study of the Interaction between Nanoplastics and Carbon Nanotubes

Granular Biomaterials as Bioactive Sponges for the Sequestration and Release of Signaling Molecules

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