Polymeric Functionalized Stationary Phase for Separation of Ionic Compounds by IC

Abstract

Synthesis and properties are described of multilayered stationary phases containing quaternary amine functional groups used for the analysis of inorganic anions by ion chromatography. The bonded phases were characterized by elemental analysis, solid state 13C NMR spectroscopy and chromatographic methods. The surface of polyhydroxyethyl methacrylate (solid support) was coated with polymeric layers formed by condensation polymerization of primary amine with diepoxide. Each layer of the anion exchange stationary phase consisted of methylamine and 1,4-butanedioldiglycidyl ether copolymer. A series of stationary phases with different number of polymerized layers were tested. Separation of inorganic anions, such as F−, Cl−, NO2−, Br−, NO3−, were performed. Aqueous hydroxide, carbonate and bicarbonate solutions were used as mobile phases.

Functionalized polymeric stationary phases for ion chromatography

“…The interpretation was difficult because the amount of added carbon during synthesis is relatively low in comparison with the carbon content present in the support. The NMR spectra confirm the presence of hydroxyl groups on the unmodified support [20].…”

“…Bare polymer after drying does not contain any nitrogen. In the previous study [20], for the same number of synthesis steps the nitrogen content was 0.14, 0.40, 0.91 and 1.35% respectively. The difference is observed especially for first seven steps.…”

“…The last two steps were repeated 3, 7, 11 and 15 times to make 3, 7, 11 and 15 layers on solid support, respectively. The detailed recipe synthesis of the stationary phases was presented earlier [20]. In the present work, 1,4DMH was used as a support.…”

“…In a previous study, polyhydroxyethyl methacrylate was used as the support for anion exchanger [20].…”

Investigation of Chromatographic Performance of Hyperbranched Amine-Modified Stationary Phases in Ion Chromatography

“…For the separation of anions, hyperbranched condensation polymers have been prepared by reacting several different diepoxides and methylamine on sulfonated ethylvinylbenzenedivinylbenzene [10,11], acrylate polymers [14,15] and silica gels [16,17]. Nucleotides have been separated on dendrimeric stationary phases [18].…”