Polymeric electrospun scaffolds for bone morphogenetic protein 2 delivery in bone tissue engineering

Aragón, Javier; Salerno, Simona; Irusta, Silvia; Mendoza, Gracia

doi:10.1016/j.jcis.2018.07.029

Cited by 57 publications

(42 citation statements)

References 52 publications

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“…25,26 Moreover, generalized osteoporosis was reported to negatively influence implant stability and implant failures are mostly due to poor bone quality. [29][30][31] Nevertheless, native proteins which are applied are prone to degrade at a relatively rapid speed in humoral environment. [29][30][31] Nevertheless, native proteins which are applied are prone to degrade at a relatively rapid speed in humoral environment.…”

Section: Discussionmentioning

confidence: 99%

“…27,28 In recent years, scaffolds combined with specific actives substance (eg vascular endothelial growth factor and bone morphogenetic protein 2) were utilized to regulate cell behaviour in acellular therapy to promote bone formation. [29][30][31] Nevertheless, native proteins which are applied are prone to degrade at a relatively rapid speed in humoral environment. 32,33 Furthermore, HA has been F I G U R E 2 Characterization of FHA.…”

Section: Bone Disorders Such As Osteoporosis Have Become One Of Healthmentioning

confidence: 99%

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Fluorine‐contained hydroxyapatite suppresses bone resorption through inhibiting osteoclasts differentiation and function in vitro and in vivo

et al. 2019

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Objectives: Fluorine, an organic trace element, has been shown to unfavourably effect osteoclasts function at a low dose. Use of hydroxyapatite (HA) has been effective in exploring its roles in promoting bone repair. In this study, we used HA modified with fluorine to investigate whether it could influence osteoclastic activity in vitro and ovariectomy-induced osteoclasts hyperfunction in vivo. Materials and methods: Fluorohydroxyapatite (FHA) was obtained and characterized by scanning electron microscope (SEM). Osteoclasts proliferation and apoptosis treated with FHA were assessed by MTT and TUNEL assay. SEM, F-actin, TRAP activity and bone resorption experiment were performed to determine the influence of FHA on osteoclasts differentiation and function. Moreover, HA and FHA were implanted into ovariectomized osteoporotic and sham surgery rats. Histology and Micro-CT were examined for further verification. Results: Fluorine released from FHA slowly and sustainably. FHA hampered osteoclasts proliferation, promoted osteoclasts apoptosis, suppressed osteoclasts differentiation and function. Experiments in vivo validated that FHA participation brought about an inhibitory effect on osteoclasts hyperfunction and less bone absorption. Conclusion:The results indicated that FHA served as an efficient regulator to attenuate osteoclasts formation and function and was proposed as a candidature for bone tissue engineering applications.

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Section: Discussionmentioning

confidence: 99%

Section: Bone Disorders Such As Osteoporosis Have Become One Of Healthmentioning

confidence: 99%

Fluorine‐contained hydroxyapatite suppresses bone resorption through inhibiting osteoclasts differentiation and function in vitro and in vivo

et al. 2019

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“…PCL membranes decorated with PLGA particles loaded with RFP were also produced using a Yflow 2.2.D-500 electrospinner (Electrospinning Machines/R&D Microencapsulation, Spain) following the protocol described in previous studies (Aragón et al, 2018). Two needles were used to obtain the PCL fibres (needle 1) and to electrospray the PLGA-RFP particles (needle 2).…”

Section: Electrospun Scaffold Productionmentioning

confidence: 99%

Composite scaffold obtained by electro-hydrodynamic technique for infection prevention and treatment in bone repair

Aragón

Feoli

Irusta

et al. 2019

International Journal of Pharmaceutics

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Bone infection is a devastating condition resulting from implant or orthopaedic surgery. Therapeutic strategies are extremely complicated and may result in serious side effects or disabilities. The development of enhanced 3D scaffolds, able to promote efficient bone regeneration, combined with targeted antibiotic release to prevent bacterial colonization, is a promising tool for the successful repair of bone defects. Herein, polymeric electrospun scaffolds composed of polycaprolactone (PCL) nanofibres decorated with poly(lactic-co-glycolic acid) (PLGA) particles loaded with rifampicin were fabricated to achieve local and sustained drug release for more efficient prevention and treatment of infection. The release profile showed an initial burst of rifampicin in the first six hours, enabling complete elimination of bacteria. Sustained and long-term release was observed until the end of the experiments (28 days), facilitating a prolonged effect on the inhibition of bacterial growth, which is in agreement with the common knowledge concerning the acidic degradation of the microparticles. In addition, bactericidal effects against gram negative (Escherichia coli) and gram positive (Staphylococcus aureus) bacteria were demonstrated at concentrations of released rifampicin up to 58 ppm after 24 h, with greater efficacy against S. aureus (13 ppm vs 58 ppm for E. coli). Cell morphology and cytocompatibility studies highlighted the suitability of the fabricated scaffolds to support cell growth, as well as their promising clinical application for bone regeneration combined with prevention or treatment of bacterial infection.

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“…This technique is often used with degradable scaffolds or hydrogels, as the protein release can be controlled by the degradation or swelling of the matrix. Several techniques have been used to encapsulate GFs in the scaffold including electrospinning, [38,39] gas foaming/particle leaching, [40] and in situ polymerization. [41] The release profile of GFs encapsulated using these techniques will depend on whether the polymer matrix undergoes surface erosion or bulk erosion.…”

Section: Conventional Controlled Release Systemsmentioning

confidence: 99%

Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

Teixeira

Domingues

Shevchuk

et al. 2020

Adv Funct Materials

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Growth factors (GFs) are proteins secreted by cells that regulate a variety of biological processes. Although they have long been proposed as potent therapeutic agents, their administration in a soluble form has proven costly and ineffective due to their short half-lives in biological environments. Biomaterial-based approaches are increasingly sought as alternatives to improve the efficacy or, ideally, replace the need for exogenous administration of GFs in regenerative medicine strategies. The means by which these systems evolve from biomaterials for conventional controlled release of GFs to the recent extracellular matrix (ECM)-inspired approaches for sequestering these labile molecules and regulating their spatiotemporal activity and presentation are reviewed. Focus is placed on biomaterials functionalized either with ECM components, which show promiscuous GF binding, or with targeted GF ligands (antibodies, aptamers, or peptides). The potential of synthetic platforms with abiotic affinity as cost-effective alternatives to the current biological ligands is also discussed. Overall, the various GF sequestering systems developed so far have remarkably improved the activity of GFs at reduced doses and, in some cases, completely avoided the need for their exogenous administration to guide cell fates. These bioinspired concepts thus enable the rational exploration of the full therapeutic potential of GFs in regenerative medicine.

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Polymeric electrospun scaffolds for bone morphogenetic protein 2 delivery in bone tissue engineering

Cited by 57 publications

References 52 publications

Fluorine‐contained hydroxyapatite suppresses bone resorption through inhibiting osteoclasts differentiation and function in vitro and in vivo

Fluorine‐contained hydroxyapatite suppresses bone resorption through inhibiting osteoclasts differentiation and function in vitro and in vivo

Composite scaffold obtained by electro-hydrodynamic technique for infection prevention and treatment in bone repair

Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

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