Polymeric cryogels as promising materials of biotechnological interest

Lozinsky, V. I.; Galaev, Igor Yu.; Plieva, Fatima M.; Savina, Irina N.; Jungvid, Hans; Mattìasson, Bo

doi:10.1016/j.tibtech.2003.08.002

Cited by 732 publications

(551 citation statements)

References 36 publications

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“…Organic nitrogen sources are directly incorporated into proteins or transformed into other cellular nitrogenous constituents [16]. Enhancement using immobilized cells of phototrophic bacteria was also reported by Singh et al (1994) [17], Zhu et al (1999) [10], Yokoi et al (1997) [18], Lozinsky et al (2003) [19], and many other workers. Immobilized, sulfur-deprived algal cultures of Chlamyodomonas reinhardtii could photoproduce hydrogen for longer periods of time [20,21].…”

Section: Resultsmentioning

confidence: 67%

Photoproduction of Hydrogen under Different Cultural Conditions by Alginate Immobilized Rhodopsedomonas palustris KU003

Merugu

Rudra

Nageshwari

et al. 2012

ISRN Renewable Energy

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Rhodopsedomonas palustris KU003 was immobilized in alginate and its hydrogen producing abilities were assessed. Maximum hydrogen production took place between 120 and 144 hrs in most carbon sources. Alginate immobilization induced higher amount of hydrogen in malate-, lactate-, and succinate-containing medium in Rps. palustris. Incubation period of 120 hrs was optimum for production of hydrogen. pH 7.0 ± 0.4 was optimum for production of hydrogen. L-glutamic acid was a good nitrogen source for production of hydrogen. Glucose and sorbitol were poorer substrates as they induced only limited amount of hydrogen. Anaerobic light induced comparatively more amount of hydrogen in the bacteria under investigation than in anaerobic dark. Thiourea was a poor nitrogen source for the production of hydrogen by Rps. palustris. Results of the above are discussed in the light of existing literature in this communication.

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Section: Resultsmentioning

confidence: 67%

Photoproduction of Hydrogen under Different Cultural Conditions by Alginate Immobilized Rhodopsedomonas palustris KU003

Merugu

Rudra

Nageshwari

et al. 2012

ISRN Renewable Energy

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“…are attractive for many applications, especially in biomedicine and biotechnology (Lozinsky, 2002;Lozinsky et al, 2003). In particular, the synthesis of cryogels via UV irradiation appears to be a very fast and effi cient approach which can be set up at low cost (Doycheva et al, 2004;Petrov et al 2006Petrov et al , 2007.…”

Section: Bn10 Cells Entrapped In Cryogelsmentioning

confidence: 99%

Biosurfactant Production by Pseudomonas aeruginosa BN10 Cells Entrapped in Cryogels

Christova

Petrov

Kabaivanova

2013

Zeitschrift Für Naturforschung C

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Production of a rhamnolipid biosurfactant by cells of Pseudomonas aeruginosa strain BN10 immobilized into poly(ethylene oxide) (PEO) and polyacrylamide (PAAm) cryogels was investigated under semicontinuous shake fl ask conditions and compared to biosurfactant secretion by free cells. The biosurfactant synthesis was followed over 9 cycles of operation of the immobilized system, each cycle comprising 7 days at ambient temperature and neutral pH. Type and quantity of the carrier were optimized for the rhamnolipid production. The highest rhamnolipid yield of 4.6 g l-1 was obtained in the 6th cycle for the immobilized system with 3 g PEO compared to 4.2 g l-1 obtained for the free cells, thus immobilization provided physiological stability of the cells. Scanning electron microscopy revealed preservation of the cell shape and regular distribution of the cells under the matrix surface. The polymer matrices possessed chemical and biological stability and very good physico-mechanical characteristics which are a prerequisite for a high life span of these materials for the production of rhamnolipids

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“…Diffusion of macromolecules in porous structures is hindered in comparison to diffusion in free solution (Schroder et al, 2006). Various supports have been used for enzymes immobilization such as synthetic organic polymers, biopolymers, hydrogels, smart polymers and inorganic Science Publications AJBB supports (Katchalski-Katzir and Kraemer, 2000;Lozinsky et al, 2003;Sheldon, 2007;Salemi, 2010). A variety of biopolymers, mainly water insoluble polysaccharides such as cellulose, starch, agarose and chitosan and proteins such as gelatin and albumin have been widely used as supports for immobilizing enzymes (Krajewska, 2004;Spahn and Minteer, 2008).…”

Section: Introductionmentioning

confidence: 99%

Potential Applications of Chitosan Nanoparticles as Novel Support in Enzyme Immobilization

Malmiri

Jahanian

Berenjian

2012

American Journal of Biochemistry and Biotechnology

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Chitosan is an attractive natural biopolymer from renewable resources with the presence of reactive amino and hydroxyl functional groups in its structure. Due to the good biocompatibility of chitosan, it can be used in magnetic-field assisted drug delivery, enzyme or cell immobilization and many other industrial applications. In the past decade, nanotechnology has been a considerable research interest in the area of preparation of immobilized enzyme carriers. This study looks at characteristics and applications of chitosan and chitosan nanoparticles and their potentials as suitable supports for enzyme immobilization. Results indicated that activity of immobilized enzymes and performance of enzyme immobilization onto chitosan nanoparticles are higher than chitosan macro and microparticles. As compared to other biopolymers nanoparticles, application of chitosan nanoparticles to immobilize enzymes strongly increases stability of immobilized enzymes and their easy separability from the reaction mixture at the end of the biochemical process.

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Polymeric cryogels as promising materials of biotechnological interest

Cited by 732 publications

References 36 publications

Photoproduction of Hydrogen under Different Cultural Conditions by Alginate Immobilized Rhodopsedomonas palustris KU003

Photoproduction of Hydrogen under Different Cultural Conditions by Alginate Immobilized Rhodopsedomonas palustris KU003

Biosurfactant Production by Pseudomonas aeruginosa BN10 Cells Entrapped in Cryogels

Potential Applications of Chitosan Nanoparticles as Novel Support in Enzyme Immobilization

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