Polymeric coatings in packed fused silica micro‐LC columns

Abstract

Abstract. Different polymercoated stationary phases have been evaluated in micro-LC: Nucleosil-100-5-Cl-PMSClS (C,,-substituted polymethylsiloxane, C1-deactivated with trimethylsilylenolate) and Nucleosil-100-5-C1-PMSCN (cyanopropyl-substituted polymethylsiloxane, Cl-deactivated) in the reversed phase mode and Nucleosil-100-5-PEG (polyethylene glycol-coated) in the normal phase mode.Fused silica tubing of 320-pm i.d. was slurry packed with suspensions optimized for each packing material. The micropacked columns were studied for efficiency and selectivity. The silanol activities of the polymer coated phases were compared to monomeric phases for the reversed phase mode and to silica for the normal phase mode.

Microcolumn liquid chromatography: instrumentation, detection and applications

“…The question whether small-I.D. LC columns show better kinetic properties compared to their conventional counterparts has been the subject of much discussion in the literature [60][61][62][63][64][65]. The results of these investigations covering different column I.D.s, and performed under test conditions which were not always similar, can be summarized as follows: h values are typically in the same range as conventional LC columns.…”