Kinetic performance of open-tubular and packed columns in LC using the same stationary phase: Immobilized polymethyloctadecylsiloxane

New trends in reversed-phase liquid chromatographic separations of therapeutic peptides and proteins: Theory and applications

“…An alternative to packed and monolithic columns may be the so-called porous-layer open-tubular-type (PLOT) columns, which have been shown to provide very high column efficiency compared to conventional packed columns [158]. Open-tubular columns for liquid chromatographic applications were first investigated by Tsuda Adapted from Ref.…”

Biomolecule Analyses in an Open-Tubular Capillary Chromatography Using Ternary Mixed Carrier Solvents with Chemiluminescence Detection

“…Most of these studies, however, used coated or modified capillary tubes. [8][9][10][11][12][13][14] We also modified the inner walls with specific functional groups or molecules, such as phenylboronic acid, iminodiacetic acid, and antibodies for capillary chromatography to take advantage of specific interactions between the solutes and the modified walls. [15][16][17][18] The coating of the inner walls required for separation in chromatography is both a time-consuming and laborious procedure.…”

Analytical Conditions and Separation Performance of Capillary Chromatography Based on the Tube Radial Distribution of Aqueous-Organic Mixture Carrier Solvents under Laminar-Flow Conditions

“…9 Porous silica structures were also introduced on the wall by dynamic or static coating techniques, [10][11][12] and various polymeric siloxanes were immobilized on the walls. 13,14 We modified the inner walls with specific functional groups or molecules, such as phenylboronic acid, iminodiacetic acid, and antibodies for capillary chromatography to take advantage of specific interactions between the solutes and the modified walls. [15][16][17][18] Methods for the preparation of open capillary tubes coated with molecularly imprinted polymer 19 and cation exchange polymer 20 have also been reported for use in chromatography.…”