Polymerase chain reaction of 2-kb cyanobacterial gene and human anti-α1-chymotrypsin gene from genomic DNA on the In-Check single-use microfabricated silicon chip

Consolandi, Clarissa; Severgnini, Marco; Frosini, Andrea; Caramenti, G.; Fazio, Marco; Ferrara, Francesco; Zocco, A.; Fischetti, A.; Palmieri, M.; Bellis, Gianluca De

doi:10.1016/j.ab.2006.03.019

Cited by 22 publications

(18 citation statements)

References 19 publications

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“…The static treatment involves the pre-coating of chip surface, during fabrication of PCR chip or immediately before use, with one of the following substances: SiO 2 (10,14,20,21,25), bovine serum albumin (BSA) (11,17,25,37,53,61,73), polyethylene glycol (PEG) (65,78,82) or silanizing agents (for example 3-glycidoxypropyl trimethoxysilane (11), dichlorodimethylsilane (23,69), Sigmacoat Õ (16,29) or trimethylchlorosilane (33). The SiO 2 pre-coating is a reproducible and inexpensive standard MEMS process and can be accomplished in a batch fashion.…”

Section: Surface Treatmentmentioning

confidence: 99%

“…This dynamic treatment occurs during the actual operation of PCR chips because the reagents are in the PCR solution. Other widely used dynamic reagents are BSA (10,11,13,16,20,21,28,31,50,51,53,57,59,60,(62)(63)(64)70,(74)(75)(76)78) and polyvinylpyrrolidone (PVP) (17,21,44). Glycerol (10,78), formamide (10,78), polyethylene glycol (PEG) 8000 (21), and Tween 20 (21), have also been studied as dynamic coating agents.…”

Section: Surface Treatmentmentioning

confidence: 99%

“…During the past two years, the miniaturization of such PCR devices has been of growing interest. In general, they can be classified as single chamber stationary PCR chip ( Figure 1A) (10,(12)(13)(14)(15)(16)18,21,22,24,26,27,29,32,37,39,40,43,45,46,48,(51)(52)(53)57,62,63,(68)(69)(70) and multi chamber stationary PCR chip ( Figure 1B) (11,17,19,20,25,28,31,(33)(34)(35)(36)38,47,49,54,78). The former can perform very well in terms of fluidic and thermal controls and offer beneficial properties such as reduced thermal and fluidic crosstalk between chambers.…”

Section: Stationary Chamber-based Pcr Amplificationmentioning

confidence: 99%

“…Among the contact heating methods, the MEMSbased film heating elements have smaller thermal mass, faster thermal response and higher heating rates (e.g. 4108C/s) (11,14,31,32,35,38,40,45,47,48,51,77,79,80,89) (see Table 1). To date, the fastest heating rate (1758C/s) and cooling rate (1258C/s) have been obtained (65) using this method (80).…”

Section: On-chip Pcr Reaction Speedmentioning

confidence: 99%

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Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends

Zhang

Xing

2007

Nucleic Acids Research

379

291

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The possibility of performing fast and small-volume nucleic acid amplification and analysis on a single chip has attracted great interest. Devices based on this idea, referred to as micro total analysis, microfluidic analysis, or simply ‘Lab on a chip’ systems, have witnessed steady advances over the last several years. Here, we summarize recent research on chip substrates, surface treatments, PCR reaction volume and speed, architecture, approaches to eliminating cross-contamination and control and measurement of temperature and liquid flow. We also discuss product-detection methods, integration of functional components, biological samples used in PCR chips, potential applications and other practical issues related to implementation of lab-on-a-chip technologies.

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Section: Surface Treatmentmentioning

confidence: 99%

Section: Surface Treatmentmentioning

confidence: 99%

Section: Stationary Chamber-based Pcr Amplificationmentioning

confidence: 99%

Section: On-chip Pcr Reaction Speedmentioning

confidence: 99%

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Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends

Zhang

Xing

2007

Nucleic Acids Research

379

291

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“…The In-Check platform has already been used successfully to amplify human genome sequences and detect human genome mutations, such as the gene associated with β-thalassemia as well as the detection of viral infectious diseases with full integration of the PCR amplification with subsequent microarray detection [22–24]. …”

Section: Introductionmentioning

confidence: 99%

Novel Biochip Platform for Nucleic Acid Analysis

Pernagallo

Ventimiglia

Cavalluzzo

et al. 2012

Sensors

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This manuscript describes the use of a novel biochip platform for the rapid analysis/identification of nucleic acids, including DNA and microRNAs, with very high specificity. This approach combines a unique dynamic chemistry approach for nucleic acid testing and analysis developed by DestiNA Genomics with the STMicroelectronics In-Check platform, which comprises two microfluidic optimized and independent PCR reaction chambers, and a sequential microarray area for nucleic acid capture and identification by fluorescence. With its compact bench-top “footprint” requiring only a single technician to operate, the biochip system promises to transform and expand routine clinical diagnostic testing and screening for genetic diseases, cancers, drug toxicology and heart disease, as well as employment in the emerging companion diagnostics market.

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Effect of oxidized silicon (SiO_x) surfaces functionalization on real‐time PCR by Lab‐on‐a‐chip microdevices

Suriano¹,

Hume²,

Cereda³

et al. 2011

Surface & Interface Analysis

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aThere is a great need to improve the biocompatibility of silicon-based lab-on-chip substrate materials for reliable quantitative analysis of biological solutions. These advanced microdevice surfaces need not only be biocompatible but also have surfaces of defined wettability characteristics. The inhibition of biomolecular activity due to microdevice surface interaction is common and can result in inaccurate results or decreased reaction yields. In this work we investigate different techniques for the chemical functionalization of oxidized silicon (SiO x ) surfaces in order to: (i) obtain defined hydrophobic/hydrophilic surfaces; and (ii) increase the efficiency of performing Real-Time Polymerase Chain Reaction (PCR) on a silicon-based lab-on-chip. Silicon oxide surfaces are functionalized by grafting alkylic chain silanes and poly(ethylene glycol) (PEG) chains to the surfaces, rendering them hydrophobic or hydrophilic. Functionalized surfaces are characterized through contact angle and atomic force microscopy (AFM) measurements, showing stable hydrophobic surfaces with contact angles of 69-78• and layer thicknesses of 11-15 Å and hydrophilic surfaces displaying contact angles of 5-6• and thicknesses of 22-52 Å. PCR experiments carried out directly on bare silicon oxide lab-on-chip surfaces show low yields of DNA amplification. Hydrophobic surfaces decrease the inhibition of PCR. Hydrophilic surfaces are a major improvement on the bare silicon oxide exhibiting the same maximum reaction yield as obtained with a standard thermocycler. We have found that the best results are associated with PEG modified surfaces, which prove very suitable for the fabrication of reliable PCR silicon lab-on-chips.

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Polymerase chain reaction of 2-kb cyanobacterial gene and human anti-α1-chymotrypsin gene from genomic DNA on the In-Check single-use microfabricated silicon chip

Cited by 22 publications

References 19 publications

Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends

Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends

Novel Biochip Platform for Nucleic Acid Analysis

Effect of oxidized silicon (SiO_x) surfaces functionalization on real‐time PCR by Lab‐on‐a‐chip microdevices

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Polymerase chain reaction of 2-kb cyanobacterial gene and human anti-α1-chymotrypsin gene from genomic DNA on the In-Check single-use microfabricated silicon chip

Cited by 22 publications

References 19 publications

Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends

Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends

Novel Biochip Platform for Nucleic Acid Analysis

Effect of oxidized silicon (SiOx) surfaces functionalization on real‐time PCR by Lab‐on‐a‐chip microdevices

Contact Info

Product

Resources

About

Effect of oxidized silicon (SiO_x) surfaces functionalization on real‐time PCR by Lab‐on‐a‐chip microdevices