Polymerase chain reaction for laboratory diagnosis of orf virus infections

Torfason, Einar G.; Guðnadóttir, Sigrún

doi:10.1016/s1386-6532(01)00232-3

Cited by 79 publications

(43 citation statements)

References 3 publications

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“…Multiplex PCR and PCR targeting B2L or virus interferon resistance gene (VIR) gene has been employed to diagnose the parapoxvirus infections (Inoshima et al, 2000;Torfason and Gunadottir, 2002;Kottaridi et al, 2006b;Abraho et al, 2012). A PCR assay developed for rapid diagnosis of contagious ecthyma virus specific primers targeting a highly conserved GIF/IL-2 and A32L gene was found sensitive to detect minimum DNA concentration of 5 ng (Ramesh et al, 2008;.…”

Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%

Contagious Pustular Dermatitis (Orf Disease) - Epidemiology, Diagnosis, Control and Public Health Concerns

Kumar¹,

Trivedi²,

Bhatt³

et al. 2015

Adv. Anim. Vet. Sci.

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| Contagious pustular dermatitis (CPD), also known as Orf or contagious ecthyma is an important viral disease of sheep and goats. It is mainly seen as a benign disease but malignant form has also been reported from few parts of the world. The rates of morbidity and mortality are higher, particularly in lambs and kids experiencing the disease for the first time. The causative agent of disease is Orf virus, type species of the genus Parapoxvirus belonging to family Poxviridae. The virus produces localized persistent proliferative skin lesions and affected hosts are infected repeatedly owing to its host immune evasive strategy. These viruses have been found uniformly labile to chloroform but resistant to ether and also exhibit antigenic variations among them. Clinically, the disease is enzootic and occurs in three forms viz. labial, genital / mammary and generalized forms. The incubation period in natural cases is 2-3 weeks. The disease outbreaks mostly occur between autumn and spring but its severity is more in autumn and winter than spring. The virus grows well in cell cultures of caprine, ovine and bovine origin. Confounding symptoms impose laboratory affirmation by serological assays or molecular techniques. The disease can be diagnosed by electron microscopy, serological tests and PCR/ quantitative real-time PCR. Virus specific antibody response to structural proteins of capripox and orf viruses in western-blot analysis readily differentiates these two infections. The antibody response to the 32 kDa and 26 kDa proteins of capripox viruses provides a firm basis for differentiation. Although, the role of humoral immunity is well established but probably cell mediated immunity plays a major role in recovery from natural infections. A number of inactivated and live or live modified vaccines have been tried with variable success. The duration of immunity after vaccination is controversial; outbreaks have occurred in vaccinated animals. The disease is also of public health significance as it causes infection in human beings. Keywords

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Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%

Contagious Pustular Dermatitis (Orf Disease) - Epidemiology, Diagnosis, Control and Public Health Concerns

Kumar¹,

Trivedi²,

Bhatt³

et al. 2015

Adv. Anim. Vet. Sci.

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“…It can cause contagious ecthyma in goats, sheep, and other ruminants worldwide. The viruses are sometimes transmissible to humans due to direct contact [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

“…Essential genes are located in the conserved central part of the genome, and variability is observed in the terminal ends [5]. Genes in near-terminal regions of the genome encode factors with important roles in viral-host interactions such as modulating host responses to infection and determining host range, but often not essential for growth in cultured cells [2,8]. The B2L gene resides in the BamHI B fragment of NZ-2 strain and encodes a major envelope protein of 42 kDa [9].…”

Section: Introductionmentioning

confidence: 99%

Identification and phylogenetic analysis of orf virus from goats in Taiwan

et al. 2007

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An outbreak of contagious ecthyma in goats in central Taiwan was investigated. The disease was diagnosed by physical and histopathologic examinations, and the etiology of the disease was identified as orf virus by electron microscopy and polymerase chain reaction (PCR) and sequence of major envelope protein (B2L) gene. The entire protein-coding region of B2L gene were cloned and sequenced. Phylogenetic analysis of B2L amino acid sequences showed that the orf virus identified in this outbreak was closer to the Indian ORFV-Mukteswar 59/05 isolate. This is the first report on the molecular characterization of orf virus in Taiwan.

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“…Ancak histopatolojik bulgular hastalığın klinik dönemine göre değişmektedir. Hastalığın tanı koydurucu histopatolojik bulgusu, piknotik epidermal hücreler, üst epidermis hücrelerinde solukluk, eozinofilik inklüzyon cisimcikleri içeren vakuolizasyon gösteren hücreler, histiyosit, makrofaj ve lenfosit infiltrasyonu 8,9 . Hastamızda tanıyı anamneze ve klinik bulgulara dayanarak koyduk.…”

Section: Olguunclassified