Identification and phylogenetic analysis of orf virus from goats in Taiwan

Chan, Kuei-Chuan; Lin, Jen‐Wei; Lee, Shu-Hwae; Liao, Chih-Jung; Tsai, Meng-Chun; Hsu, Wei‐Li; Wong, Min‐Liang; Shih, Hong-Mo

doi:10.1007/s11262-007-0144-6

Cited by 66 publications

(55 citation statements)

References 22 publications

(34 reference statements)

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“…Virus isolation was confirmed by conventional PCR targeting the full length major enveloped protein gene, which resulted an amplification of B2L gene with an expected DNA fragment of 1206 bp of Orf virus. These results matched the results of orf viruses reported earlier from different parts of India and throughout the world [2,3,6,15].…”

supporting

confidence: 92%

Isolation and molecular characterization of Orf virus from natural outbreaks in goats of Assam

Bora

Barman

et al. 2015

VirusDis.

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Outbreaks of contagious ecthyma (caused by a Parapox virus) in goats were investigated in 6 districts of Assam, a north eastern state of India. Diagnosis of the disease was carried out employing both standard virological as well as molecular methods. Four representative isolates from different places were selected for phylogenetic analysis. The major envelop protein (B2L) of Orf virus was targeted for molecular analysis. The sequencing and phylogenetic analysis of the selected sequences at nucleotide level revealed that the Orf virus isolates were closely related to each other (97.6-100 %) and showed highest similarity to the Orf virus isolate 82/04 (98.4 %), reported from Shahjahanpur, India. The data will provide an insight in transmission of the virus from northern to North eastern part of the country.

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supporting

confidence: 92%

Isolation and molecular characterization of Orf virus from natural outbreaks in goats of Assam

Bora

Barman

et al. 2015

VirusDis.

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“…ORFVs from the same outbreak have been reported [1,5,6,12,14,24]. Guo et al reported an outbreak of ORFV infection in a zoo, in which a single ORFV likely infected musk ox (Ovibos moschatus), domestic Shetland sheep (Ovis aries) and Sichuan takin (Budorcas taxicolor tibetana), but the amino acid sequence of the viral envelope gene contained one or two substitutions in each animal [12].…”

Section: Discussionmentioning

confidence: 95%

“…Sequence analyses of the viral envelope gene indicate that contagious pustular dermatitis in Japanese serows is mainly caused by ORFV [18,19]. However, only a partial sequence of the envelope gene was analyzed in these studies, and in other countries, sequence variations occur in the envelope gene of ORFVs isolated from the same outbreaks [1,5,6,12,14,24]. In general, the amino acid sequences of the genes encoding viral structural proteins, such as the envelope, are relatively well conserved, but the detailed genetic relationship between the PPVs of the recent and earlier outbreaks in Japanese serows is unclear.…”

mentioning

confidence: 99%

Spatial and Temporal Genetic Homogeneity of Orf Viruses Infecting Japanese Serows (Capricornis crispus)

Inoshima

Ito

Ishiguro

2010

The Journal of Veterinary Medical Science

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ABSTRACT. This study genetically characterized orf viruses (ORFVs) isolated from recent outbreaks in Japanese serows (Capricornis crispus) in 2007 and 2008, and from earlier outbreaks from 1985 to 2001. Nucleotide sequences of genes for the viral envelope, vascular endothelial growth factor (VEGF), and virus interferon resistance (VIR) were determined in two ORFVs isolated from recent outbreaks and in eight from earlier outbreaks. No deletions or insertions were observed in these genes. Surprisingly, the amino acid sequences of the envelope and VIR genes were identical among the 10 ORFVs, and only one amino acid substitution in the VEGF gene was found in one of the two recent ORFVs (2007). Genotyping by differential PCR for the A32L gene, which encodes an ATPase, classified all of the 10 ORFVs into the same genotype. In an ORFV isolated from a sheep in 1970, the three sequenced genes were almost the same as in the ORFVs isolated from Japanese serows, and the ORFV in 1970 was classified into the same genotype as the ORFVs from Japanese serows. These results suggest that recent outbreaks in Japanese serows are caused by ORFVs genetically closely related to the viruses in earlier outbreaks and that these genetically stable ORFVs have circulated among Japanese serows over a long period of time.

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“…covering a raised area of ulceration, granulation and inflammation (1). The diagnosis can be made by clinical examination of the lesions, but histopathology, PCR and ELISA methods are also used.…”

mentioning

confidence: 99%

Comparison of effectiveness of parenteral lincomycin/spectinomycin combination and dexpanthenol application in goat kids with contagious ecthyma

Kasap

Temizel

Karakuş

et al. 2018

Medycyna Weterynaryjna

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Contagious ecthyma (CE) is a highly contagious viral skin disease that is typically self-limited. Treatment options include topical antiseptics, such as KMNO4, local antibiotics and systemic antibiotics to prevent secondary skin infections. The aim of this study was to compare the effectiveness of the lincomycin/spectinomycin combination and dexpanthenol (Dxp) in goat kids with CE. The study was conducted at a Saanen dairy goat farm in Bursa, Turkey. The owner of the goat herd inquired at the veterinary hospital about the appearance of granulomatous lesions on the muzzles of goat kids. In this study, 24 goat kids (1-month-olds) were used. All animals were subjected to the same conditions. Blood and papule samples were taken from the animals’ lips, muzzle and buccal for virological analysis. Swab samples were taken from the lesions for culture and antibiogram. The animals were divided into three groups. Lesions were clinically scored at days 1, 7 and 15 according to a modified previously used scoring system. Goat kids were equally grouped on the basis of lesions on the buccal, lips and muzzle. The animals in group A received 15 mg/kg lincomycin/spectinomycin combination (Lypectin®, Vilsan) intramuscularly for 3 consecutive days, group B received 20 mg/kg Dxp (Bepanthen® amp, Bayer, Germany) intramuscularly for 3 consecutive days, and group C received 0.9% NaCl (2 ml), the control treatment. Clinical recoveries were almost equal in all groups, but by day 14, group A showed better recovery than group B and group C. Both study groups also showed better results than the control group for all days. In conclusion, we believe that the lincomycin/spectinomycin combination or dexpanthenol can be useful in the supplementary treatment of CE in goat kids..

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Identification and phylogenetic analysis of orf virus from goats in Taiwan

Cited by 66 publications

References 22 publications

Isolation and molecular characterization of Orf virus from natural outbreaks in goats of Assam

Isolation and molecular characterization of Orf virus from natural outbreaks in goats of Assam

Spatial and Temporal Genetic Homogeneity of Orf Viruses Infecting Japanese Serows (Capricornis crispus)

Comparison of effectiveness of parenteral lincomycin/spectinomycin combination and dexpanthenol application in goat kids with contagious ecthyma

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