POLYMERASE CHAIN REACTION DETECTION OF &lt;i&gt;PASTEURELLA MULTOCIDA&lt;/i&gt; TYPE B: 2 IN MICE INFECTED WITH CONTAMINATED RIVER WATER

Abdullah, Faez Firdaus Jesse; Khaleel, Mohammed Muqdad; Adamu, Lawan; Osman, Abdinasir Yusuf; Haron, Abdul Wahid; Saad, Mohd Zamri; Omar, Abdul Rahman

doi:10.3844/ajavsp.2013.146.151

Cited by 9 publications

(9 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Rivers and streams have been thought to be responsible for the transmission of haemorrhagic septicemia through carcasses washed down from one village upstream to another further down the rivers (Khaleel et al, 2013). In fact HS has been associated with the monsoon seasons (Abdullah et al, 2013a) and dumping of infected carcasses into streams and rivers. Khaleel et al (2014) Stated that there is dissemination and multiplication of P. multocida B: 2 in vital organs after intranasal and subcutaneous challenge in mice.…”

Section: Introductionmentioning

confidence: 99%

Hormonal and Histopathological Alterations in Pituitary Glands and Reproductive Organs of Male and Female Mice Orally Inoculated with Pasteurella Multocida Type B: 2 and its Lipopolysaccharides

Abdullah¹,

Adamu²,

Tijjani³

et al. 2015

American Journal of Animal and Veterinary Sciences

View full text Add to dashboard Cite

Oral infections of mice as animal model with Pasteurella multocida type B: 2 and its lipopolysaccharides is liable to cause detrimental alterations in the pituitary glands, which results in disruption of reproductive hormonal levels as well as organ functions in the male and female animals; thereby impeding the fertility of at risk animal population. Therefore, this study was designed to evaluate the histopathological modifications in the pituitary glands, reproductive organs and the associated hormonal levels in male and female mice inoculated orally with P. multocida type B: 2 and its lipopolysaccharides. Forty eight mice were divided into three groups consisting of sixteen mice each (8 male and 8 female in separate cages). Group 1 served as the control group and were inoculated orally with 0.4 mL Phosphate Buffer Saline (PBS), group 2 were inoculated orally with 0.4 mL of 10 9 Colony Forming Unit (CFU) of P. multocida type B: 2 while group 3 were inoculated orally with 0.4 mL of LPS from 10 9 of colony P. multocida type B: 2. There were significant differences (p<0.05) in the lesions (oedema, haemorrhage, degeneration and necrosis) observed in the testes, ovaries and the pituitary glands amongst the three groups. Similarly, there were significant differences in the levels of testosterone, progesterone and estrogen (p<0.05) in the male and female mice inoculated orally with P. multocida type B: 2 and its lipopolysaccharide. There is paucity of information on changes related to reproductive hormones, pituitary glands and reproductive organs of male and female mice following oral inoculation with P. multocida type B: 2 and its endotoxin. Hence, the sex hormonal levels could be used to assess the immune statuses of at risk animal population and proffer amenable Veterinary medical care to afflicted animal population.

show abstract

Section: Introductionmentioning

confidence: 99%

Hormonal and Histopathological Alterations in Pituitary Glands and Reproductive Organs of Male and Female Mice Orally Inoculated with Pasteurella Multocida Type B: 2 and its Lipopolysaccharides

Abdullah¹,

Adamu²,

Tijjani³

et al. 2015

American Journal of Animal and Veterinary Sciences

View full text Add to dashboard Cite

show abstract

“…In another study in the same area, the highest prevalence of HS disease was in a canal irrigated area of Rahim Yar Khan District in Punjab (Khan, ). It was also postulated recently that HS outbreaks among cattle and buffaloes could be due to using contaminated river water with HS carcasses (Abdullah et al., ).…”

Section: Discussionmentioning

confidence: 99%

A Case-control Study of Haemorrhagic Septicaemia in Buffaloes and Cattle in Karachi, Pakistan, in 2012

Moustafa

Ali

Bennett

et al. 2015

Transbound Emerg Dis

View full text Add to dashboard Cite

Summary A retrospective epidemiological case–control study was performed in Karachi, Pakistan, from January to April 2013. The owners of 217 dairy cattle and buffalo farms from six different locations in Karachi were interviewed. The aim of the study was to identify risk factors associated with the presence of haemorrhagic septicaemia (HS). Farms with a history of at least one instance of sudden death in a dairy animal during 2012 and a positive clinical HS diagnosis (made by local veterinarians) were defined as cases. Farms having no history of sudden deaths in 2012 were defined as controls. Univariable analyses were initially conducted, and factors with P ≤ 0.25 were offered to a multivariable logistic regression model to identify putative risk factors. The final multivariable logistic model contained five factors. Vaccination was found to be a protective factor (OR = 0.22) along with the length of time cattle were kept on farm (months). For every extra month cattle were kept, the odds of HS disease were reduced by a factor of 0.9. In contrast, for every extra animal in a herd, the risk of infection increased by a factor of 1.01. Supplying underground water and the presence of foot and mouth disease on the farm increased the risk by 2.90 and 2.37, respectively. To understand the epidemiology of HS in Karachi dairy herds, more in‐depth research is required to study the risk and protective factors identified in this survey and to evaluate risk mitigation strategies, where possible.

show abstract

“…It was observed that bacteria was shed into the water and was successfully recovered and inoculated into susceptible mice through various routes of inoculation. Earlier, Abdullah et al (2013b) had reported the successful recovery of P. multocida using PCR from various organs of mice inoculated with contaminated river water. HS is a deadly infection and the extent of lesion manifestation depends on the duration of the disease and the dose of bacterial inoculums (Kharb and Charan, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…The disease is caused by serotypes B and E strains of Pasteurella multocida (Kharb and Charan, 2012;Khaleel et al, 2013;Abdullah et al, 2013a;. HS is widespread in most parts of tropical Asia and Africa.…”

Section: Introductionmentioning

confidence: 99%

HISTOPATHOLOGICAL CHANGES IN MICE INFECTED WITH RIVER WATER CONTAMINATED BY PASTEURELLA MULTOCIDA TYPE B:2

Khaleel

Abdullah

Adamu

et al. 2014

American Journal of Animal and Veterinary Sciences

View full text Add to dashboard Cite

Haemorrhagic Septicaemia (HS) is a fatal systemic disease of bovines typified by an acute, highly fatal septicaemic disease with high morbidity and mortality. In this study, sixty five adult ICR mice were used for the study. In the first phase, 5 mice each were inoculated with 10 9 cfu of P. multocida type B:2 through the intraperitoneal route. After development of classical signs of HS, the mice were euthanised and dropped into a tank of river waterfor 24, 48 and 72 h. Contaminated water from the tank were collected at the three different times (24, 48 and 72 h) and was used for intraperitoneal, oral and aerosol inoculation of 15 mice each with a dose of 1 mL −1 10 9 cfu (intraperitoneal and aerosol) and 0.4 mL −1 10 9 cfu (oral). The control group (n = 5) was given phosphate buffered saline. All mice in the three groups and control were culled after 72 h post inoculation and the vital organs (liver, spleen, lung, kidney, heart, brain) were collected in 10% buffered formalin, procesed, sectioned and stained with H&E. Degeneration and necrosis, hemorrhage and infiltration of inflammatory cells were the most observed lesions in all the organs, while thrombosis and oedema were least observed. The lesion severity was moderate to severe using contaminated water at 72 h post contamination and mild to moderate when inoculated with water 24 and 48 h post contamination. Mice inoculated through the oral and intraperitoneal routes recorded more severe lesion compared with those inoculated through the aerosol route. However, using contaminated water at 24 h, mice inoculated through the aerosol route had the highest distribution of inflammatory cells in the lungs, which declined following inoculation with contaminated water at 48 and 72 h, respectively.This study has shown that contamination of water bodiesby carcasses of HS infected animals is a likely source of infection to heathy animals in the wild and that development of lesions severity is time dependant and significantly higher using contaminated water at 72 h than water contaminated for 24 and 48 h. This further indicates the danger of leaving decomposing carcasses in water bodies for prolonged periods.

show abstract

POLYMERASE CHAIN REACTION DETECTION OF <i>PASTEURELLA MULTOCIDA</i> TYPE B: 2 IN MICE INFECTED WITH CONTAMINATED RIVER WATER

Cited by 9 publications

References 9 publications

Hormonal and Histopathological Alterations in Pituitary Glands and Reproductive Organs of Male and Female Mice Orally Inoculated with <i>Pasteurella Multocida</i> Type B: 2 and its Lipopolysaccharides

Hormonal and Histopathological Alterations in Pituitary Glands and Reproductive Organs of Male and Female Mice Orally Inoculated with <i>Pasteurella Multocida</i> Type B: 2 and its Lipopolysaccharides

A Case-control Study of Haemorrhagic Septicaemia in Buffaloes and Cattle in Karachi, Pakistan, in 2012

HISTOPATHOLOGICAL CHANGES IN MICE INFECTED WITH RIVER WATER CONTAMINATED BY <i>PASTEURELLA MULTOCIDA </i>TYPE B:2

Contact Info

Product

Resources

About