HISTOPATHOLOGICAL CHANGES IN MICE INFECTED WITH RIVER WATER CONTAMINATED BY &lt;i&gt;PASTEURELLA MULTOCIDA &lt;/i&gt;TYPE B:2

Khaleel, Mohammed Muqdad; Abdullah, Faez Firdaus Jesse; Adamu, Lawan; Abba, Yusuf; Haron, Abdul Wahid; Saad, Mohd Zamri; Omar, Abdul Rahman

doi:10.3844/ajavsp.2014.71.76

Cited by 11 publications

(9 citation statements)

References 6 publications

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“…In fact HS has been associated with the monsoon seasons (Abdullah et al, 2013a) and dumping of infected carcasses into streams and rivers. Khaleel et al (2014) Stated that there is dissemination and multiplication of P. multocida B: 2 in vital organs after intranasal and subcutaneous challenge in mice.…”

Section: Introductionmentioning

confidence: 99%

Hormonal and Histopathological Alterations in Pituitary Glands and Reproductive Organs of Male and Female Mice Orally Inoculated with <i>Pasteurella Multocida</i> Type B: 2 and its Lipopolysaccharides

Abdullah¹,

Adamu²,

Tijjani³

et al. 2015

American Journal of Animal and Veterinary Sciences

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Oral infections of mice as animal model with Pasteurella multocida type B: 2 and its lipopolysaccharides is liable to cause detrimental alterations in the pituitary glands, which results in disruption of reproductive hormonal levels as well as organ functions in the male and female animals; thereby impeding the fertility of at risk animal population. Therefore, this study was designed to evaluate the histopathological modifications in the pituitary glands, reproductive organs and the associated hormonal levels in male and female mice inoculated orally with P. multocida type B: 2 and its lipopolysaccharides. Forty eight mice were divided into three groups consisting of sixteen mice each (8 male and 8 female in separate cages). Group 1 served as the control group and were inoculated orally with 0.4 mL Phosphate Buffer Saline (PBS), group 2 were inoculated orally with 0.4 mL of 10 9 Colony Forming Unit (CFU) of P. multocida type B: 2 while group 3 were inoculated orally with 0.4 mL of LPS from 10 9 of colony P. multocida type B: 2. There were significant differences (p<0.05) in the lesions (oedema, haemorrhage, degeneration and necrosis) observed in the testes, ovaries and the pituitary glands amongst the three groups. Similarly, there were significant differences in the levels of testosterone, progesterone and estrogen (p<0.05) in the male and female mice inoculated orally with P. multocida type B: 2 and its lipopolysaccharide. There is paucity of information on changes related to reproductive hormones, pituitary glands and reproductive organs of male and female mice following oral inoculation with P. multocida type B: 2 and its endotoxin. Hence, the sex hormonal levels could be used to assess the immune statuses of at risk animal population and proffer amenable Veterinary medical care to afflicted animal population.

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Section: Introductionmentioning

confidence: 99%

Hormonal and Histopathological Alterations in Pituitary Glands and Reproductive Organs of Male and Female Mice Orally Inoculated with <i>Pasteurella Multocida</i> Type B: 2 and its Lipopolysaccharides

Abdullah¹,

Adamu²,

Tijjani³

et al. 2015

American Journal of Animal and Veterinary Sciences

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“…The bacteria multiply in peritonsilar soft tissue, and macrophages may also carry viable organisms to local lymph nodes, where they multiply and spread to surrounding tissues (Wilkie et al 2012). However, virulence factors related to adherence and cellinvasive capacity found only in P. multocida B:2, rather than other serotypes like to P. multocida A:3, were thought to play an important role in the disease pathogenesis, suggesting that P. multocida B:2 has ability to translocate from the respiratory tract into the blood stream and produce its effect in other sites (Othman et al 2012;Annas et al 2014;Khaleel et al 2014). Moreover, the LPS as a major virulence factor of P. multocida B:2 has been reported to induce direct effect in male and female reproductive organs of mice in addition to disturbance in the levels of testosterone, estrogen and progesterone, respectively .…”

Section: Discussionmentioning

confidence: 98%

“…The cellular changes observed were haemorrhage and congestion, necrosis and degeneration, inflammatory cell infiltration and oedema. The histopathological changes were recorded and an overall scoring for severity was assigned to each sample as follows: score 0, normal, score 1, mild; score 2, moderate; score 3, severe (Khaleel et al 2014;Jesse et al 2015).…”

Section: Histopathological Examinationmentioning

confidence: 99%

Molecular detection and pathology of Pasteurella multocida B:2 in the reproductive system of pre-pubertal buffalo calves (Bubalus bubalis)

et al. 2015

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Pasteurella multocida is responsible for one of the major animal diseases with economic importance in both developed and developing countries. P. multocida B:2 causes haemorrhagic septicemia (HS) in cattle and buffaloes, which result in heavy economic losses through direct effect, resulting in high mortality rates and indirect effects through impairment of the animal productivity. It is well known that HS affects mainly the respiratory and digestive tracts of host animals. However, involvement of the reproductive system in the pathogenesis of HS has not been reported previously. This study was designed to present detailed detection and histopathological changes of the reproductive system and mammary glands of buffaloes experimentally infected with P. multocida B:2. Briefly, nine healthy buffalo calves aged 8-month-old were divided into three groups of three calves each. Calves from groups 1 and 2 were inoculated subcutaneously and orally with 10 mL of 1×10 12 cfu/mL of P. multocida B:2, respectively, while calves of group 3 were inoculated orally with 10 mL of phosphate buffer saline as a sham control. All the calves in group 1 had to be euthanized after 12 h post-infection, while calves in group 2 were euthanized after day 21 post-infection. Successful isolation and PCR confirmation of P. multocida B:2 was achieved from different parts of the reproductive system, including ovary, oviduct, uterine horn, uterine body and vagina as well as mammary glands and supramammary lymph nodes of the buffaloes in group 1 after 12 h post-infection. However, P. multocida B:2 was not detected in the reproductive organs of buffaloes in group 2 and group 3 after 21 days post-infection. Significant differences (P<0.05) were found in histopathological changes between the two groups, which mainly affected different anatomic regions of the reproductive system. This work provides an insight into the involvement of the female reproductive system of buffaloes during the pathogenesis of HS and shows that route of inoculation strongly affect the localization of the bacterium in the reproductive system.

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“…Lesion severity was thus interpreted as mild, moderate, or severe based on a previous scoring system reported by Khaleel et al (2014).…”

Section: Histopathological Lesion Evaluationmentioning

confidence: 99%

Gonado-hypophyseal lesions and reproductive hormonal changes in Brucella melitensis-infected mice and its lipopolysaccharides (LPSs)

et al. 2015

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Gonado-hypophyseal lesions and reproductive hormonal changes in Brucella melitensis-infected mice and its lipopolysaccharide (LPS) were investigated. Forty-eight mice (24 males and 24 females) were used as infection models in this study, and they were divided into three equal separate groups of eight mice based on their sex: M1, M2, and M3 and F1, F2, and F3 (M=Male, F=Female). Groups M1 and F1 were orally inoculated with sterile phosphate-buffered saline (PBS) and served as the negative control. Groups M2 and F2 were orally inoculated with 0.4 ml of 10 9 colony-forming units (cfu) of B. melitensis, while groups M3 and F3 were orally inoculated with 0.4 ml of LPSs from 10 9 cfu of B. melitensis. All the groups were observed for 10 days for changes in clinical signs or signs of mortality and then euthanized. Whole blood was collected via cardiac puncture, while tissues were fixed in 10 % formalin for histopathological processing. Histopathological lesions in the male and female reproductive tracts ranged from mild to moderate degenerative, inflammatory, and necrotic lesions. These changes were moderate in M2 than in M3 in males and in F3 than in F2 groups in females. Lesions in the pituitary gland ranged from mild to moderate inflammation and necrosis with astrogliosis. F2 and M2 groups had more lesion distribution in the pituitary gland than F3 and M3 groups. There was a 13 % decline in progesterone concentration in F2 and a 44 % decline in F3. Estradiol level declined by 33 % in F2 and slightly increased by 4.2 % in F3. Progesterone-estrogen ratio (P/E) increased by 31 % in F2 and declined by 46 % in F3. Testosterone concentration increased by 74 % in M2 and decreased by 56 % in M3. The study showed that oral administration of B. melitensis affects the pituitary gland and may alter the hypothalamicpituitary-gonadal axis.

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HISTOPATHOLOGICAL CHANGES IN MICE INFECTED WITH RIVER WATER CONTAMINATED BY <i>PASTEURELLA MULTOCIDA </i>TYPE B:2

Cited by 11 publications

References 6 publications

Hormonal and Histopathological Alterations in Pituitary Glands and Reproductive Organs of Male and Female Mice Orally Inoculated with <i>Pasteurella Multocida</i> Type B: 2 and its Lipopolysaccharides

Hormonal and Histopathological Alterations in Pituitary Glands and Reproductive Organs of Male and Female Mice Orally Inoculated with <i>Pasteurella Multocida</i> Type B: 2 and its Lipopolysaccharides

Molecular detection and pathology of Pasteurella multocida B:2 in the reproductive system of pre-pubertal buffalo calves (Bubalus bubalis)

Gonado-hypophyseal lesions and reproductive hormonal changes in Brucella melitensis-infected mice and its lipopolysaccharides (LPSs)

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