Polymerase chain reaction-based biochemical logic gate coupled with cell-free transcription–translation of green fluorescent protein as a report gate

Abstract

Polymerase chain reaction-based biochemical logic gates were designed for AND, OR, NOT, and AND-NOT operations, whose output signal is reported by coupled cell-free transcription-translation of green fluorescent protein.

Nucleotides and Nucleic Acids; Oligo- and Polynucleotides

“…The pH driven transition between single strand and i-motif of cytosine rich oligonucleotides has been used as a simple on-off switch motor. 683 RNA based logic gates (AND, NOR, NAND, OR) capable of executing cellular processes based on aptamers, 488,684 riboswitches 438 and a transcription-translation system 685 have been reported. A photochemical logic gate has been designed based on the photochemical ligation via 5-carboxyvinyluridine and photochemical cleavage by carbazole-modified oligonucleotides.…”

Growing prospects of DNA nanomaterials in novel biomedical applications

“…The inputs of DNA/RNA, metal ions or light will activate the logic gates and then produce output signals based on Watson-Crick base-pairing, DNA strand displacement, DNAzyme cleavage, 127 nucleic acid ligand binding, 128 i-motif transformation, DNA hairpins 129 and PCR. 130 Ghadiri et al 131 successfully created three photonic logic gates, AND, NAND and INHIBIT, using the unique base-pairing properties of DNA and FRET between uorescent molecules. Subsequently, ssDNA was exploited to invade and displace local sections of complementary dsDNA.…”

Nerve Gas Simulant Sensing by a Uranyl–Salen Monolayer Covalently Anchored on Quartz Substrates

“…[4][5][6][7][8][9][10][11][12][13] Molecular complementarity betweent he sensing agent andt arget compound assures selective analyte recognition. [14][15][16][17][18][19][20] Nevertheless,t he design of effectives ensing agents that retain and possibly enhance the molecular recognition properties at the solid-state interfacec ontinues to be am ajor challenge. [21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36] The development of device-quality monolayerbased sensors requires not only selectivity and sensitivity towardsaspecific analyte, but also ah igh degree of stability and af ast nondestructive read-outprocess.…”

“…Molecular films for sensing applications recently attracted much academic and industrial interest . Molecular complementarity between the sensing agent and target compound assures selective analyte recognition …”