Polymer surfaces structured with random or aligned electrospun nanofibers to promote the adhesion of blood platelets

Wan, Ling‐Shu; Xu, Zuhua

doi:10.1002/jbm.a.31907

Cited by 33 publications

(20 citation statements)

References 48 publications

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“…The NF surfaces had higher surface FIB adsorption compared to PCL and NW surfaces resulting in higher platelet adhesion. This is consistent with recent work demonstrating higher platelet adhesion and activation on NF surfaces [67]. …”

Section: Resultssupporting

confidence: 94%

Hemocompatibility of polymeric nanostructured surfaces

Leszczak

Smith

Popat

2013

Journal of Biomaterials Science, Polymer Edition

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Tissue integration is an important property when inducing transplant tolerance, however, the hemocompatibility of the biomaterial surface also plays an important role in the ultimate success of the implant. Therefore, in order to induce transplant tolerance, it is critical to understand the interaction of blood components with the material surfaces. In this study, we have investigated the adsorption of key blood serum proteins, in vitro adhesion and activation of platelets and clotting kinetics of whole blood on flat polycaprolactone (PCL) surfaces, nanowire (NW) surfaces and nanofiber (NF) surfaces. Previous studies have shown that polymeric nanostructured surfaces improve cell adhesion, proliferation and viability; however it is unclear how these polymeric nanostructured surfaces interact with the blood and its components. Protein adsorption results indicate that while there were no significant differences in total albumin adsorption on PCL, NW and NF surfaces, NW surfaces had higher total fibrinogen and immunoglobulin-G adsorption compared to NF and PCL surfaces. In contrast, NF surfaces had higher surface FIB and IgG adsorption compared to PCL and NW surfaces. Platelet adhesion and viability studies show more adhesion and clustering of platelets on the NF surfaces as compared to PCL and NW surfaces. Platelet activation studies reveal that NW surfaces have the highest percentage of unactivated platelets, whereas NF surfaces have the highest percentage of fully activated platelets. Whole blood clotting results indicate that NW surfaces maintain an increased amount of free hemoglobin during the clotting process compared to PCL and NF surface, indicating less clotting and slower rate of clotting on their surfaces.

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Section: Resultssupporting

confidence: 94%

Hemocompatibility of polymeric nanostructured surfaces

Leszczak

Smith

Popat

2013

Journal of Biomaterials Science, Polymer Edition

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“…Through results obtained from the images, it could be interpreted that nanostructured surfaces promoted the activation and adhesion of platelets. Wan and Xu in their study showed that the presence of nanofibers enhanced the activation and adhesion of platelets and induced the changes in surface topography and charge . Furthermore, SF‐coated Vit K/BC/CTS and PS/BC/CTS have more platelet attachment compared to other composite BC/CTS hemostatic dressings.…”

Section: Resultsmentioning

confidence: 97%

“…Wan and Xu in their study showed that the presence of nanofibers enhanced the activation and adhesion of platelets and induced the changes in surface topography and charge. 34 Furthermore, SF-coated Vit K/BC/CTS and PS/BC/CTS have more platelet attachment compared to other composite BC/CTS hemostatic dressings. Conversely, these groups showed less LDH activity compared to SF or SF/PC-coated BC/CTS dressings.…”

Section: Platelet Adhesionmentioning

confidence: 97%

Active nano/microbilayer hemostatic agents for diabetic rat bleeding model

et al. 2016

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Patients with diabetes mellitus have an increased cardiovascular risk due to the abnormality of hemostatic system components. Therefore, hemostasis is an important concept when considering that diabetics are under risk due to potential bleeding complications during surgical operation. The aim of our study was to examine the efficiency of a fabricated nano/microbilayer hemostatic dressing for bleeding control in diabetic patients. For this purpose, we prepared a nano/microbilayer hemostatic dressing that has a porous sublayer, including chitosan (CTS), bacterial cellulose (BC) as basement and active agents in coagulation cascade, such as vitamin K (Vit K), protamine sulfate (PS), and kaolin (Kao) as a filler and an upper layer consisting of silk fibroin (SF) or SF/phosphatidylcholine (PC) blend to achieve complete hemostasis in diabetic rats. Coagulative performances of the prepared hemostatic dressings were examined by the determination of bleeding time, blood loss, and mortality rate through diabetic rat femoral artery injury model. The percent of diabetic rat blood absorption was found to be 247 ± 5% for gauze as opposed to 2214 ± 56% for SF-coated PS/BC/CTS. Vit K-reinforced within 138 s and SF-coated BC/CTS hemostatic dressings within 144 s showed a rapid coagulation time. In vivo coagulation studies demonstrated that hemostatic agent-reinforced BC/CTS hemostatic dressing, especially PS/BC/CTS showed a significant hemostatic plug formation. This study suggests that the high positive charge and porosity give to these hemostatic agents reinforced hemostatic dressings the ability to rapidly swell and to promote the accumulation of red blood cells and platelets through electrostatic interactions. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1573-1585, 2017.

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“…To this end, electrospun nanofibrous scaffolds with various alignments (e.g. axially aligned, yarn, and radially aligned) have shown superior capacity in shaping cell morphology, guiding cell migration, and affecting cell differentiation when compared to other types of scaffolds both in vitro and in vivo 48,53,54 . More significantly, specific cellular behaviors (e.g.…”

Section: Biomimetic Nanofibrous Scaffolds For Tissue Engineeringmentioning

confidence: 99%

Biomimetic electrospun nanofibrous structures for tissue engineering

2013

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Biomimetic nanofibrous scaffolds mimicking important features of the native extracellular matrix provide a promising strategy to restore functions or achieve favorable responses for tissue regeneration. This review provides a brief overview of current state-of-the-art research designing and using biomimetic electrospun nanofibers as scaffolds for tissue engineering. It begins with a brief introduction of electrospinning and nanofibers, with a focus on issues related to the biomimetic design aspects. The review next focuses on several typical biomimetic nanofibrous structures (e.g. aligned, aligned to random, spiral, tubular, and sheath membrane) that have great potential for tissue engineering scaffolds, and describes their fabrication, advantages, and applications in tissue engineering. The review concludes with perspectives on challenges and future directions for design, fabrication, and utilization of scaffolds based on electrospun nanofibers.

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Polymer surfaces structured with random or aligned electrospun nanofibers to promote the adhesion of blood platelets

Cited by 33 publications

References 48 publications

Hemocompatibility of polymeric nanostructured surfaces

Hemocompatibility of polymeric nanostructured surfaces

Active nano/microbilayer hemostatic agents for diabetic rat bleeding model

Biomimetic electrospun nanofibrous structures for tissue engineering

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