Polymer Shelled Microparticles for a Targeted Doxorubicin Delivery in Cancer Therapy

Cerroni, Barbara; Chiessi, Ester; Margheritelli, Silvia; Oddo, Letizia; Paradossi, Gaio

doi:10.1021/bm101207k

Cited by 68 publications

(57 citation statements)

References 55 publications

(84 reference statements)

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“…HA in 3D scaffolds induces the growth of spherical tumoroids during the proliferative steps . We observed that HA is a determining factor for internalization of poly(vinyl alcohol) microparticles coated with HA as targeting agent of HT‐29 cells . Versatile and multiresponsive hybrid HAAz/p(NiPAAm) constructs can be suitably designed as nanoparticles, highly selective for tumor cells thus enabling specific drug delivery.…”

Section: Resultsmentioning

confidence: 88%

Multiresponsive Hyaluronan‐p(NiPAAm) “Click”‐Linked Hydrogels

Pasale

Cerroni

Ghugare

et al. 2014

Macromolecular Bioscience

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Combined reversible addition-fragmentation chain transfer (RAFT) and chemoselective "click" chemistry are used for assembling two polymeric chains into a hybrid network capable to respond simultaneously or separately to different external stimuli. An azido-derivative of hyaluronate is clicked together with a new telechelic RAFT-generated p(NiPAAm), carrying a propargyl function at both ends, suitable as macromolecular "clickable" cross-linker with controlled molecular weight. This hybrid system displays a multiresponsive behavior versus temperature, pH, and ionic strength, maintaining cumulative as well as separate sensitivities to the external stimuli. Hyaluronidase catalyzed degradation of the hydrogels, mimicking the extracellular matrix degradation process, is an additional asset for the use of this class of hydrogels as scaffold. Tumor cells, HT-29, grow on the surface of these hybrid hydrogels more than the healthy ones, as NIH3T3. This finding opens a road to micro- and nano-devices based on hyaluronic acid as a promising biopolymer to pursue localized drug delivery.

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Section: Resultsmentioning

confidence: 88%

Multiresponsive Hyaluronan‐p(NiPAAm) “Click”‐Linked Hydrogels

Pasale

Cerroni

Ghugare

et al. 2014

Macromolecular Bioscience

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“…Recently, polyvinyl alcohol (PVA) capsules gained specific interest due to their chemical versatility that enables functionalisation with different ligands, for instance hyaluronic acid for the targeting of tumour cells and tissues [90]. The properties of the air-filled PVA capsules were compared to other polymer-coated capsules and commercially available bubbles with lipid and protein coatings [91].…”

Section: Other Types Of Targeted Tucamentioning

confidence: 99%

Targeted ultrasound contrast agents for ultrasound molecular imaging and therapy

Rooij

Daeichin

Skachkov

et al. 2015

International Journal of Hyperthermia

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Ultrasound contrast agents (UCAs) are used routinely in the clinic to enhance contrast in ultrasonography. More recently, UCAs have been functionalised by conjugating ligands to their surface to target specific biomarkers of a disease or a disease process. These targeted UCAs (tUCAs) are used for a wide range of pre-clinical applications including diagnosis, monitoring of drug treatment, and therapy. In this review, recent achievements with tUCAs in the field of molecular imaging, evaluation of therapy, drug delivery, and therapeutic applications are discussed. We present the different coating materials and aspects that have to be considered when manufacturing tUCAs. Next to tUCA design and the choice of ligands for specific biomarkers, additional techniques are discussed that are applied to improve binding of the tUCAs to their target and to quantify the strength of this bond. As imaging techniques rely on the specific behaviour of tUCAs in an ultrasound field, it is crucial to understand the characteristics of both free and adhered tUCAs. To image and quantify the adhered tUCAs, the state-of-the-art techniques used for ultrasound molecular imaging and quantification are presented. This review concludes with the potential of tUCAs for drug delivery and therapeutic applications.

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“…29 The MBs and microgels also have the capability to act as a delivery vehicle for drugs or cytostatic agents. 32,33 Several attempts have been made to improve drug delivery systems in order to enhance stability, pharmacokinetics, bioavailability and biodistribution for several compounds. 34 For example, Villa et al have shown that MBs can be used for drug delivery of doxorubicin to cancer cells.…”

Section: Introductionmentioning

confidence: 99%

Dynamic MR Imaging, Biodistribution and Pharmacokinetics of Polymer Shelled Microbubbles Containing Spion

Barrefelt

Paradossi

Asem

et al. 2014

NANO

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Magnetic Resonance Imaging (MRI) is a noninvasive diagnostic method that provides information on morphological and physiological changes of the internal organs over time. Imaging and measurements can be repeated on the same subject, thereby reducing inter-individual variability effects and hence the number of subjects required. A potential MRI contrast agent consisting of microbubbles embedded with superparamagnetic iron oxide nanoparticles (SPION) in the shell (SPION MBs) was injected intravenously into rats to determine their biodistribution and pharmacokinetics using MR imaging. Agarose phantoms containing SPION MBs were scanned using 3 T MRI to construct a standard curve. Rats were injected with SPION MBs, free SPION or plain MBs and scanned dynamically at 3 T using a clinical MR scanner. The relaxation rate (R2*) was studied over time as a measure of the iron oxide concentrations to enable calculation of the pharmacokinetic parameters. The kinetics of SPION MBs in the liver was fitted to a one-compartment model. Furthermore, the biological fate of SPION MBs was examined via a histological survey of tissue samples using Perls' Prussian blue staining and immunohistochemistry (IHC). 1.2 h after injection of SPION MBs, T2* of the liver had decreased to its minimum. The elimination half-life of SPION MBs was 598.2 ± 97.3 h, while the half-life for SPION was 222.6 ± 26.4 h. Moreover, our study showed that SPION MBs were taken up by the macrophages in the lungs, spleen and liver. MBs labeled with SPION can be used for MR imaging. Moreover, MRI is a reliable and noninvasive tool that can be utilized in pharmacokinetic investigations of future contrast agents using SPION MBs and SPION in the rat.

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Polymer Shelled Microparticles for a Targeted Doxorubicin Delivery in Cancer Therapy

Cited by 68 publications

References 55 publications

Multiresponsive Hyaluronan‐p(NiPAAm) “Click”‐Linked Hydrogels

Multiresponsive Hyaluronan‐p(NiPAAm) “Click”‐Linked Hydrogels

Targeted ultrasound contrast agents for ultrasound molecular imaging and therapy

Dynamic MR Imaging, Biodistribution and Pharmacokinetics of Polymer Shelled Microbubbles Containing Spion

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