Polymer Particles by Templating of Vesicles

Hotz, Jutta; Meier, Wolfgang

doi:10.1002/(sici)1521-4095(199811)10:16<1387::aid-adma1387>3.0.co;2-3

Cited by 65 publications

(49 citation statements)

References 12 publications

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“…It was shown that encapsulation of QDs into liposomes provides transfer of waterinsoluble QD into aqueous media, facilitates their bioconjugation with proteins, minimizes nonspecific interaction of water-soluble and water-insoluble QD with surface material, and amplifies the analytical signal [2]. A major drawback of liposomes is their instability in vivo and during storage as well as their high sensitivity towards external influences such as variation in temperature, pH and osmotic pressure [26], that can however be overcome through coverage or templating them with different materials, such as a polymer [27] or silica [25]. The most important is that QDs obtained through this approach have substantially increased hydrodynamic diameter which limit their application in bio-imaging and targeting [28].…”

Section: Hydrophilization Of Qdsmentioning

confidence: 99%

Bioconjugation of quantum dots: Review & impact on future application

Foubert

Beloglazova

Rajković

et al. 2016

TrAC Trends in Analytical Chemistry

117

110

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Nowadays luminescent semiconductor quantum dots (QDs) are widely applied in different areas due to their unique optical properties. QDs can be used as photoluminescent labels with excellent possibilities for high-throughput detection and diagnostics. For most of such applications QDs must be coupled to biomolecules, which often represents a fundamental challenge. Although QDs have a lot of advantages over organic dyes, most of the techniques that have been developed for QD functionalization and bioconjugation, are more complicated than the corresponding techniques for organic fluorescent dyes. Here, the importance of choosing a suitable bioconjugation strategy in different applications, such as imaging and assays is described. The main goal of this review is to give a structured and detailed overview and comparison of the most widely used conjugation strategies in function of the active groups (carboxyl, amine, thiol, epoxy, hydroxyl and aldehyde groups) present on QD surface.

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Section: Hydrophilization Of Qdsmentioning

confidence: 99%

Bioconjugation of quantum dots: Review & impact on future application

Foubert

Beloglazova

Rajković

et al. 2016

TrAC Trends in Analytical Chemistry

117

110

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“…It has been shown that the hydrophobic portion of lipid bilayers can be selectively swollen by a variety of hydrophobic monomers, such as styrene [37,38,40,41], alkyl(meth)acrylates [39,42,43] or even lipofullerenes carrying polymerizable octadecadiine side chains [44]. A cross-linking polymerization of such monomers leads to the formation of two-dimensional polymer networks in the interior of the membranes.…”

Section: 3mentioning

confidence: 99%

Colloidal Nanoreactors and Nanocontainers

Sauer

Meier

2003

Colloids and Colloid Assemblies

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“…The possible osmosis of QDs through the phospholipid bilayer would results in luminescence decrease, which is undesirable for immunolabel. Leakage of the phospholipid membrane can be halted through the coverage of liposomes with a polymer net [27,28] or silica cover [29,30]. Silica coverage prevents the aggregation of liposomes and the leakage of their content, increasing their stability and facilitating their desiccation and therefore their storage.…”

Section: Introductionmentioning

confidence: 99%

Silica-coated liposomes loaded with quantum dots as labels for multiplex fluorescent immunoassay

Beloglazova¹,

Goryacheva²,

Speranskaya³

et al. 2015

Talanta

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In: TALANTA, 134, 120-125, 2015. To refer to or to cite this work, please use the citation to the published version:Beloglazova N., Goryacheva O., Speranskaya E., Aubert T., Shmelin P. , Kurbangaleev V., Goryacheva I.Y., De Saeger S. (2015). Silica-coated liposomes loaded with quantum dots as labels for multiplex fluorescent immunoassay. TALANTA, 134,[120][121][122][123][124][125] AbstractThis manuscript describes synthesis and followed application of silica-coated liposomes loaded with quantum dots as a perspective label for immunoaasay. The hollow spherical structure of liposomes makes them an attractive package material for encapsulation of multiple water-insoluble quantum dots and amplifying the analytical signal. Silica coverage ensures the stability of the loaded liposomes against fusion and internal leakage during storage, transporting, application and also provides groups for bioconugation. For the first time these nanostructures were employed for the sensitive multiplex immunochemical determination of two analytes. As a model system mycotoxins zearalenone and aflatoxin B1 were detected in cereals. For simplification of multiassay results' evaluation the silanized liposomed loaded with QDs of different colors were used. The IC 50 values for the simultaneous determination of zearalenone and aflatoxin B1 were 16.2 and 18 µg kg -1 for zearalenone and 2.2 and 2.6 µg kg -1 for aflatoxin B1 in wheat and maize, respectively. As confirmatory method, liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was used.

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Polymer Particles by Templating of Vesicles

Cited by 65 publications

References 12 publications

Bioconjugation of quantum dots: Review & impact on future application

Bioconjugation of quantum dots: Review & impact on future application

Colloidal Nanoreactors and Nanocontainers

Silica-coated liposomes loaded with quantum dots as labels for multiplex fluorescent immunoassay

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