Polymer-Coated Magnetite Nanoparticles for Protein Immobilization

Abstract

Since their discovery, magnetic nanoparticles (MNPs) have become materials with great potential, especially considering the applications of biomedical sciences. A series of works on the preparation, characterization, and application of MNPs has shown that the biological activity of such materials depends on their size, shape, core, and shell nature. Some of the most commonly used MNPs are those based on a magnetite core. On the other hand, synthetic biopolymers are used as a protective surface coating for these nanoparticles. This review describes the advances in the field of polymer-coated MNPs for protein immobilization over the past decade. General methods of MNP preparation and protein immobilization are presented. The most extensive section of this article discusses the latest work on the use of polymer-coated MNPs for the physical and chemical immobilization of three types of proteins: enzymes, antibodies, and serum proteins. Where possible, the effectiveness of the immobilization and the activity and use of the immobilized protein are reported. Finally, the information available in the peer-reviewed literature and the application perspectives for the MNP-immobilized protein systems are summarized as well.

Polymeric Composite of Magnetite Iron Oxide Nanoparticles and Their Application in Biomedicine: A Review

“…An appropriate surface coating has the potential to enable targeting capabilities of IONPs, promoting the affinity of these NPs toward a specific area [28,32]. MNPs coated with polymers have a relevant application in protein immobilization by adsorption and covalent bonding [33]. Exploring the nanoparticle shape, NPs could be helpful to other applications such as magnetic biosensor systems, magnetic particle imaging (MPI), and cell separation [34].…”

“…The use of organic and inorganic molecules such as polymers, surfactants, or biomolecules has been studied as protecting agents from stabilizing the nanomaterial, avoiding aggregation, and tolerating changes in the pH and electrolytes presence be able to obtain an adequate chemistry surface [157]. The polymer could be one of the best options by the chemical and thermal stability of the NPs, because it reduces the tendency of aggregation and increases the possibilities of use [33]. However, the use of polymeric coating as protecting agents results in the increment of NPs size and, consequently, a lower magnetic property.…”

Simulação Da Concentração De Matéria Orgânica Em Canais Urbanos: Estudo De Caso Canal Do Prado, Campina Grandepb

“…Cerca de 90% das metodologias sintéticas que promovem a obtenção de nanopartículas compreendem os métodos químicos, nos quais estão contempladas as técnicas de microemulsão, hidrotermais, co-precipitação e solvotermais. Estas, diferem-se quanto a morfologia, tamanho, cristalinidade e estabilidade química das nanopartículas (MAJIDI et al, 2016;MYLKIE et al, 2021).…”

Effective utilization of magnetic nano-coupled cloned β-xylanase in saccharification process

“…22 The binding of bio-molecules like proteins, enzymes and antibodies onto magnetic nanoparticles are generally performed through covalent bonding, surface adsorption, inclusion in a gel phase, encapsulation and cross-linking by bi-functional reagents. 23–25 A recent method comprising carbodiimide activation has gain popularity because of its high efficiency and simplicity. 26…”