Polylysine-decorated macroporous microcarriers laden with adipose-derived stem cells promote nerve regeneration in vivo

Sun, Yi; Chi, Xiaoqi; Meng, Hui; Ma, Mengjiao; Wang, Jing; Feng, Zhipeng; Qi, Qi; Liu, Guodong; Wang, Yansen; Xie, Yu; Peng, Jiang

doi:10.1016/j.bioactmat.2021.03.029

Cited by 35 publications

(25 citation statements)

References 69 publications

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“…The macroprous structures on the surface of PLCL films were obtained after leaching of the NaCl porogen particles ( Supplementary Figure S1 ). The porous structures with a suitable pore size were beneficial for cell loading and adhesion, which is consistent with previous report ( Sun et al, 2021 ). The 3D and porous structures offer a larger surface area, enabling the efficient grafting/loading of KHI peptides and NGF.…”

Section: Discussionsupporting

confidence: 92%

Micropatterned Poly(D,L-Lactide-Co-Caprolactone) Conduits With KHI-Peptide and NGF Promote Peripheral Nerve Repair After Severe Traction Injury

Xing

Zhang

Liu

et al. 2021

Front. Bioeng. Biotechnol.

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Severe traction injuries after stretch to peripheral nerves are common and challenging to repair. The nerve guidance conduits (NGCs) are promising in the regeneration and functional recovery after nerve injuries. To enhance the repair of severe nerve traction injuries, in this study KHIFSDDSSE (KHI) peptides were grafted on a porous and micropatterned poly(D,L-lactide-co-caprolactone) (PLCL) film (MPLCL), which was further loaded with a nerve growth factor (NGF). The adhesion number of Schwann cells (SCs), ratio of length/width (L/W), and percentage of elongated SCs were significantly higher in the MPLCL-peptide group and MPLCL-peptide-NGF group compared with those in the PLCL group in vitro. The electromyography (EMG) and morphological changes of the nerve after severe traction injury were improved significantly in the MPLCL-peptide group and MPLCL-peptide-NGF group compared with those in the PLCL group in vivo. Hence, the NGCs featured with both bioactive factors (KHI peptides and NGF) and physical topography (parallelly linear micropatterns) have synergistic effect on nerve reinnervation after severe traction injuries.

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Section: Discussionsupporting

confidence: 92%

Micropatterned Poly(D,L-Lactide-Co-Caprolactone) Conduits With KHI-Peptide and NGF Promote Peripheral Nerve Repair After Severe Traction Injury

Xing

Zhang

Liu

et al. 2021

Front. Bioeng. Biotechnol.

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Section: Adscs In Regenerative Therapies: An Overview Of Recent Advan...mentioning

confidence: 99%

“…(a) Representative images and hematoxylin and eosin (H&E) staining of the regenerated nerve tissue at the midportion of the nerve guide conduits (NGCs) or autograft 12 weeks after implantation.Yellow arrows indicate blood vessels. Reproduced under terms of the CC-BY license [202]. Copyright 2021, The Authors, Published by Elsevier.…”

mentioning

confidence: 99%

An Update on Adipose‐Derived Stem Cells for Regenerative Medicine: Where Challenge Meets Opportunity

Qin

Yang

et al. 2023

Advanced Science

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Over the last decade, adipose‐derived stem cells (ADSCs) have attracted increasing attention in the field of regenerative medicine. ADSCs appear to be the most advantageous cell type for regenerative therapies owing to their easy accessibility, multipotency, and active paracrine activity. This review highlights current challenges in translating ADSC‐based therapies into clinical settings and discusses novel strategies to overcome the limitations of ADSCs. To further establish ADSC‐based therapies as an emerging platform for regenerative medicine, this review also provides an update on the advancements in this field, including fat grafting, wound healing, bone regeneration, skeletal muscle repair, tendon reconstruction, cartilage regeneration, cardiac repair, and nerve regeneration. ADSC‐based therapies are expected to be more tissue‐specific and increasingly important in regenerative medicine.

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“…Biodegradable materials, such as polycaprolactone, chitosan, and polylactic acid, are used to fabricate NGCs in many studies ( Barroca et al, 2018 ; Qian et al, 2020 ; Sun et al, 2021 ; Zeng et al, 2021 ). Electrical signals play an important role in normal nerve function and nerve regeneration ( Qian et al, 2019 ; Qian et al, 2020 ; Wang et al, 2020 ; Kasper et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Ti3C2Tx MXene-Coated Electrospun PCL Conduits for Enhancing Neurite Regeneration and Angiogenesis

Nan

Lin

Wang

et al. 2022

Front. Bioeng. Biotechnol.

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An electrical signal is the key basis of normal physiological function of the nerve, and the stimulation of the electric signal also plays a very special role in the repair process of nerve injury. Electric stimulation is shown to be effective in promoting axonal regeneration and myelination, thereby promoting nerve injury repair. At present, it is considered that electric conduction recovery is a key aspect of regeneration and repair of long nerve defects. Conductive neural scaffolds have attracted more and more attention due to their similar electrical properties and good biocompatibility with normal nerves. Herein, PCL and MXene-PCL nerve guidance conduits (NGCs) were prepared; their effect on nerve regeneration was evaluated in vitro and in vivo. The results show that the NGCs have good biocompatibility in vitro. Furthermore, a sciatic nerve defect model (15 mm) of SD rats was made, and then the fabricated NGCs were implanted. MXene-PCL NGCs show similar results with the autograft in the sciatic function index, electrophysiological examination, angiogenesis, and morphological nerve regeneration. It is possible that the conductive MXene-PCL NGC could transmit physiological neural electric signals, induce angiogenesis, and stimulate nerve regeneration. This paper presents a novel design of MXene-PCL NGC that could transmit self-originated electric stimulation. In the future, it can be combined with other features to promote nerve regeneration.

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Polylysine-decorated macroporous microcarriers laden with adipose-derived stem cells promote nerve regeneration in vivo

Cited by 35 publications

References 69 publications

Micropatterned Poly(D,L-Lactide-Co-Caprolactone) Conduits With KHI-Peptide and NGF Promote Peripheral Nerve Repair After Severe Traction Injury

Micropatterned Poly(D,L-Lactide-Co-Caprolactone) Conduits With KHI-Peptide and NGF Promote Peripheral Nerve Repair After Severe Traction Injury

An Update on Adipose‐Derived Stem Cells for Regenerative Medicine: Where Challenge Meets Opportunity

Ti3C2Tx MXene-Coated Electrospun PCL Conduits for Enhancing Neurite Regeneration and Angiogenesis

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