Polyion-sensitive membrane-based electrodes for heparin-binding foldamer analysis

Kelly, Caitlin; Zhan, Kevin Y.; Vena, April; Yu, Andrew; Esson, J.

doi:10.1016/j.aca.2010.09.012

Cited by 9 publications

(7 citation statements)

References 31 publications

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“…However, some foldamers bind more favorably to Lovenox than others (i.e., some foldamers possess relatively low equilibrium binding constants with heparin). Therefore, for some foldamer-Lovenox titrations the dissociation rate of the foldamer-Lovenox complex may be high enough to provide free Lovenox chains that are able to partition into a polyanion sensitive polyion sensor, eliciting a negative response much sooner than expected . It is likely that this same phenomenon is occurring during the PQ-2 titrations with DS shown in Figure c.…”

Section: Resultsmentioning

confidence: 96%

“…This is most likely due to a significantly decreased equilibrium binding constant between DS and PQ-2. Indeed, it has been documented previously that low molecular weight heparin (Lovenox) can bind to polycationic foldamers in blood plasma which serves to reverse the clinical effects of Lovenox . However, some foldamers bind more favorably to Lovenox than others (i.e., some foldamers possess relatively low equilibrium binding constants with heparin).…”

Section: Resultsmentioning

confidence: 99%

“…Indeed, it has been documented previously that low molecular weight heparin (Lovenox) can bind to polycationic foldamers in blood plasma which serves to reverse the clinical effects of Lovenox. 19 However, some foldamers bind more favorably to Lovenox than others (i.e., some foldamers possess relatively low equilibrium binding constants with heparin). Therefore, for some foldamer-Lovenox titrations the dissociation rate of the foldamer-Lovenox complex may be high enough to provide free Lovenox chains that are able to partition into a polyanion sensitive polyion sensor, eliciting a negative response much sooner than expected.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

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Characterization and Quantification of Polyquaterniums via Single-Use Polymer Membrane-Based Polyion-Sensitive Electrodes

Ferguson

Meyerhoff

2017

ACS Sens.

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Two facile, robust, and universal methods by which various polymeric quaternary ammonium salts (polyquaterniums (PQs)) can be quantified and characterized using simple potentiometric polymeric membrane polyion-sensitive electrodes as detectors are described. The two methods are (a) direct detection with polycation sensitive membrane electrodes based on the sodium salt of dinonylnaphthalenesulfonate (NaDNNS), and (b) indirect detection using polyanion sensors based on tridodecylmethylammonium chloride (TDMAC) and dextran sulfate (DS) as a titrant to complex the various polyquaternary species (four different PQs: PQ-2, PQ-6, PQ-10, and poly(2-methacryloxyethyltrimethylammonium) chloride (PMETAC)). Direct detection yields information regarding the charge density of the polycationic species. For the titration method, a series of polyanion sensors doped with TDMAC are used to follow a potentiometric titration of a PQ species using a syringe pump to deliver the titrant. This indirect detection method is more reliable and yields limits of detection in the ppm range for the four PQs examined. The titration method is further explored for detecting excess levels of PQ-6, a common flocculating agent for municipal water supply systems, within the purified water emitted by the Ann Arbor, MI, drinking water treatment plant.

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Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

Section: ■ Experimental Sectionmentioning

confidence: 99%

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Characterization and Quantification of Polyquaterniums via Single-Use Polymer Membrane-Based Polyion-Sensitive Electrodes

Ferguson

Meyerhoff

2017

ACS Sens.

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“…17 Other small molecular systems have also been reported, such as surfen, delparantag, and foldamers. 18−21 Owing to their multivalency effects, cationic oligopeptides, 22,23 synthetic polymers, 24−27 and self-assembled systems 28−31 have drawn attention.…”

Section: Introductionmentioning

confidence: 99%

Serum Albumin–Peptide Conjugates for Simultaneous Heparin Binding and Detection

et al. 2019

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Heparin is a polysaccharide-based anticoagulant agent, which is widely used in surgery and blood transfusion. However, overdosage of heparin may cause severe side effects such as bleeding and low blood platelet count. Currently, there is only one clinically licensed antidote for heparin: protamine sulfate, which is known to provoke adverse effects. In this work, we present a stable and biocompatible alternative for protamine sulfate that is based on serum albumin, which is conjugated with a variable number of heparin-binding peptides. The heparin-binding efficiency of the conjugates was evaluated with methylene blue displacement assay, dynamic light scattering, and anti-Xa assay. We found that multivalency of the peptides played a key role in the observed heparin-binding affinity and complex formation. The conjugates had low cytotoxicity and low hemolytic activity, indicating excellent biocompatibility. Furthermore, a sensitive DNA competition assay for heparin detection was developed. The detection limit of heparin was 0.1 IU/mL, which is well below its therapeutic range (0.2–0.4 IU/mL). Such biomolecule-based systems are urgently needed for next-generation biocompatible materials capable of simultaneous heparin binding and sensing.

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“…One of the most commonly used polyion-sensitive electrodes is the electrode responsive to protamine, a small polycationic protein isolated from fish sperm. Beyond the direct detection of protamine, protamine-sensitive electrodes (PrSEs) have many applications in clinical diagnosis and pharmaceutical analysis (Dai et al, 1999;Kelly et al, 2010). Based on the specific proteolysis property of protease toward protamine, PrSEs have been used to determine the activities of proteases, which play crucial roles in a number of biological and physiological processes such as digestion, immune response, blood clotting and reproduction (Hedstrom, 2002).…”

Section: Introductionmentioning

confidence: 99%

A moving-part-free protamine-sensitive polymeric membrane electrode for sensitive biomedical analyses

Wang

Qin

2012

Biosensors and Bioelectronics

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Polyion-sensitive membrane-based electrodes for heparin-binding foldamer analysis

Cited by 9 publications

References 31 publications

Characterization and Quantification of Polyquaterniums via Single-Use Polymer Membrane-Based Polyion-Sensitive Electrodes

Characterization and Quantification of Polyquaterniums via Single-Use Polymer Membrane-Based Polyion-Sensitive Electrodes

Serum Albumin–Peptide Conjugates for Simultaneous Heparin Binding and Detection

A moving-part-free protamine-sensitive polymeric membrane electrode for sensitive biomedical analyses

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