A moving-part-free protamine-sensitive polymeric membrane electrode for sensitive biomedical analyses

Wang, Xuewei; Wang, Qing; Qin, Wei

doi:10.1016/j.bios.2012.05.020

Cited by 15 publications

(7 citation statements)

References 35 publications

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“…Since the response toward polycations is based on their ion pairing interaction with DNNS, fragments digested from polycations and polycations neutralized by polyanions should exhibit smaller optical responses than intact polycations. Based on this principle, polycation-sensitive sensors can be used to indirectly detect activities of enzymes such as trypsin, − chymotrypsin, and thrombin, as well as polyanions such as heparin, , pentosan polysulfate, carrageenan, and nucleic acid . Herein, we explored the sensing application of the plasticizer-free polycation optode for both enzyme activity and polyanion detection.…”

Section: Resultsmentioning

confidence: 99%

“…The determination of trypsin activity in blood or urine is important for diagnosis of pancreatic diseases like pancreatitis and cystic fibrosis, and prognosis of pancreas transplant surgery. , A trypsin assay could also be used for screening of trypsin inhibitors as drugs. ,, Therefore, trypsin sensing was chosen as an initial example of an enzyme activity assay using the new polycation optode system. Trypsin is able to digest arginine-rich protamine into small fragments via cleaving the carboxyl side of arginine .…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, it is interesting to explore if such polyion recognition and sensing chemistry could be adapted onto the surface of paper strips, without need for a separate bulk plasticizer or polymer phase. Moreover, any new sensing scheme for polyions can enable applications for enzyme activity testing, − enzyme inhibitor screening, ,, nonseparation immunoassay, and label-free aptamer-based sensing. ,, Therefore, herein, we examine the feasibility of preparing paper-based optical polyion sensors without added plasticizer/polymer as a new cost-effective platform for various polyion sensing applications, especially for potential use in resource-poor settings.…”

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confidence: 99%

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Inkjet-Printed Paper-Based Colorimetric Polyion Sensor Using a Smartphone as a Detector

2017

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The first paper-based polyion-sensitive optodes are reported. Dinonylnaphthalene sulfonic acid (a cation exchanger) and chromoionophore I (a lipophilic optical pH indicator) are printed on filter paper in the absence of any plasticizer and/or additional hydrophobic polymeric phase. The resulting optodes exhibit sensitive colorimetric response to polycations such as protamine but not to small inorganic cations because only polycations are able to form cooperative ion pairs with dinonylnaphthalenesulfonate adsorbed to the cellulose paper. The color change of the optode is recorded via an iPhone camera and analyzed by an iPhone App. The protamine-sensing optode platform is used to indirectly detect protease activity (trypsin) based on proteolytic digestion of protamine, and polyanions (pentosan polysulfate and heparin) based on the strong binding reaction of polyanions with protamine. The indirect sensing system is further simplified on a multilayer membrane device that consists of an optode paper site modified with buffer to prevent optode dependence on sample pH, and an underlying cellulose acetate filter membrane coated with protamine to eliminate addition of the indicator polycation into the sample. The detection of pentosan polysulfate concentrations in an undiluted urine sample is successfully demonstrated via this approach. Lastly, it is shown that plasticizer-free polyanion-sensitive optodes based on an adsorbed layer of quaternary ammonium type anion exchanger and a phenolic azo type proton chromoionophore can also be fabricated directly on cellulose paper strips.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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Inkjet-Printed Paper-Based Colorimetric Polyion Sensor Using a Smartphone as a Detector

2017

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“…9,18,21 More recently, potentiometric polyion sensing has also been further developed through its application to potentiometric aptasensing, 22,23 biomedical analyses without the use of moving components, 24 and the reversible potentiometric detection of biomedically relevant polyions. 25–28 At the same time, PQ-10 has found increasing use in cosmetic applications such as shampoo formulations for its ability to act as a conditioner to aid in repairing damaged hair.…”

Section: Discussionmentioning

confidence: 99%

Detecting levels of polyquaternium-10 (PQ-10) via potentiometric titration with dextran sulphate and monitoring the equivalence point with a polymeric membrane-based polyion sensor

2016

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Polymeric quaternary ammonium salts (polyquaterniums) have found increasing use in industrial and cosmetic applications in recent years. More specifically, polyquaternium-10 (PQ-10) is routinely used in cosmetic applications as a conditioner in personal care product formulations. Herein, we demonstrate the use of potentiometric polyion-sensitive polymeric membrane-based electrodes to quantify PQ-10 levels. Mixtures containing both PQ-10 and sodium lauryl sulfate (SLS) are used as model samples to illustrate this new method. SLS is often present in cosmetic samples that contain PQ-10 (e.g., shampoos, etc.) and this surfactant species interferes with the polyion sensor detection chemistry. However, it is shown here that SLS can be readily separated from the PQ-10/SLS mixture by use of an anion-exchange resin and that the PQ-10 can then be titrated with dextran sulphate (DS). This titration is monitored by potentiometric polyanion sensors to provide equivalence points that are directly proportional to PQ-10 concentrations.

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“…

signals from the generation of ionic products or the consumption of ionic substrates in enzymatic reactions, various enzymes such as cholinesterase, [14] galactosidase, [15] and protease [16] have been indirectly detected by ion-selective SPMEs. However, for peroxidase-catalyzed reactions, ion-selective SPMEs are rarely used primarily owing to the lack of ionic substrates and products detectable by SPMs.

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confidence: 99%

Tetra(p‐tolyl)borate‐Functionalized Solvent Polymeric Membrane: A Facile and Sensitive Sensing Platform for Peroxidase and Peroxidase Mimetics

Wang

Qin

2013

Chemistry A European J

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The determination of peroxidase activities is the basis for enzyme‐labeled bioaffinity assays, peroxidase‐mimicking DNAzymes‐ and nanoparticles‐based assays, and characterization of the catalytic functions of peroxidase mimetics. Here, a facile, sensitive, and cost‐effective solvent polymeric membrane‐based peroxidase detection platform is described that utilizes reaction intermediates with different pKa values from those of substrates and final products. Several key but long‐debated intermediates in the peroxidative oxidation of o‐phenylenediamine (o‐PD) have been identified and their charge states have been estimated. By using a solvent polymeric membrane functionalized by an appropriate substituted tetraphenylborate as a receptor, those cationic intermediates could be transferred into the membrane from the aqueous phase to induce a large cationic potential response. Thus, the potentiometric indication of the o‐PD oxidation catalyzed by peroxidase or its mimetics can be fulfilled. Horseradish peroxidase has been detected with a detection limit at least two orders of magnitude lower than those obtained by spectrophotometric techniques and traditional membrane‐based methods. As an example of peroxidase mimetics, G‐quadruplex DNAzymes were probed by the intermediate‐sensitive membrane and a label‐free thrombin detection protocol was developed based on the catalytic activity of the thrombin‐binding G‐quadruplex aptamer.

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A moving-part-free protamine-sensitive polymeric membrane electrode for sensitive biomedical analyses

Cited by 15 publications

References 35 publications

Inkjet-Printed Paper-Based Colorimetric Polyion Sensor Using a Smartphone as a Detector

Inkjet-Printed Paper-Based Colorimetric Polyion Sensor Using a Smartphone as a Detector

Detecting levels of polyquaternium-10 (PQ-10) via potentiometric titration with dextran sulphate and monitoring the equivalence point with a polymeric membrane-based polyion sensor

Tetra(p‐tolyl)borate‐Functionalized Solvent Polymeric Membrane: A Facile and Sensitive Sensing Platform for Peroxidase and Peroxidase Mimetics

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