PolyGuanine methacrylate cryogels for ribonucleic acid purification

Abstract

The isolation and purification of ribonucleic acid have attracted attention recently for the understanding of the functions in detail because of the necessity for the treatment of genetic diseases. In this study, guanine-incorporated polymeric cryogels were developed to obtain highly purified ribonucleic acid. The satisfactory purification performance was achieved with the guanine-incorporated poly (2-hydroxyethyl methacrylate-guanine methacrylate) cryogels. The most crucial advantages to use guanine as a functional monomer are to obtain a real natural interaction between guanine on the polymeric material and cytosine on the ribonucleic acid. Moreover, using cryogel with a highly porous structure and high swelling ratio provide advantages of getting more water within the structure to get more analyte to interact. The characterization of cryogels has proved the success of the synthesis and the perfect natural interaction to be taken place between the ligand (guanine methacrylate) and the cytosine in the ribonucleic acid molecules. Although the pores within the structure of cryogels are small, they provide efficient and fast adsorption. The chromatographic separation performance was investigated for different conditions (pH, temperature etc.). The desorption ratio and reusability were also analyzed at the end of the five adsorption-desorption cycles with no significant changes.

The Adsorption of Calmoduline via Nicotinamide Immobilized Poly(HEMA-GMA) Cryogels

“…In the case of elemental analysis results, the poly(HEMA-GMA)@Nic-Ca(II) cryogels have the N% value corresponding the ligand amount of 32.9 mg/g cryogel (nicotinamide 1.175 mmol/g cryogel). The surface area of cryogels was estimated as 8.736 m 2 /g which quite comparable with the literature (24,25). The Ca(II) amount immobilized on the cryogels was found as 455 mmoles/g.…”

Lead Removal from Industrial Waste

“…Because of cryogelic polymerization, there were big cavities formed as a result of water removal at the end of the polymerization. The cavities with rough surface (approximately 3.343 m2/g, via Quantachrome AutosorbVR iQ-Chemi, Florida, USA, which was comparable with the literature [26][27][28]) suitable for the adsorption of lead ions could be seen it SEM (Carl Zeiss AG-EVO® 50 Series, Germany) images given in Figure 2. The water uptake amount of cryogels was found (376% on average) using the difference between the weights of dry and water retained cryogel according to the formula given by Equation 1.…”

Cryogels and Monoliths: Promising Tools for Chromatographic Purification of Nucleic Acids

“…Moreover, RNA adsorbed onto this new cryogel could be efficiently recovered without denaturation, enabling fast, cost-effective, and single-step RNA purification. While the removal of impurities from crude samples was not explicitly demonstrated, the novel cryogel showed promising results in selective and efficient RNA purification [ 125 ].…”