Polyglycerol-functionalized nanodiamond as a platform for gene delivery: Derivatization, characterization, and hybridization with DNA

Zhao, Li; Nakae, Yuki; Qin, Hongmei; Ito, Tadamasa; Kimura, Takeshi; Kojima, Hideto; Chan, Lawrence; Komatsu, Naoki

doi:10.3762/bjoc.10.64

Cited by 53 publications

(53 citation statements)

References 22 publications

(28 reference statements)

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“…For instance, this strategy has been used to coat the surface of NDs with polymers such as polyglycerol (PG) which can be formed by ring opening polymerization directly on the surface of NDs in the presence of the monomer glycidol. Furthermore, this polymer coating can be modified by converting the terminal hydroxyl groups of PG into azide (N 3 ) groups which can serve as a linking point for click chemistry reactions with a polypeptide carrying alkyne groups in the side chain [67]. …”

Section: Synthesis and Functionalization Of Ndsmentioning

confidence: 99%

“…Alternatively, Zhao et al . investigated the covalent attachment of polypeptides to polyglycerol (PG)-modified NDs to create highly-dispersed and positively charged conjugates [123]. Through click chemistry, positively-charged polypeptides were chemically conjugated with PG-NDs to form DNA vectors that were well-dispersed in solution.…”

Section: Delivery Of Therapeuticsmentioning

confidence: 99%

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Multifunctional nanodiamonds in regenerative medicine: Recent advances and future directions

Whitlow

Pacelli

Paul

2017

Journal of Controlled Release

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With recent advances in the field of nanomedicine, many new strategies have emerged for diagnosing and treating diseases. At the forefront of this multidisciplinary research, carbon nanomaterials have demonstrated unprecedented potential for a variety of regenerative medicine applications including novel drug delivery platforms that facilitate the localized and sustained release of therapeutics. Nanodiamonds (NDs) are a unique class of carbon nanoparticles that are gaining increasing attention for their biocompatibility, highly functional surfaces, optical properties, and robust physical properties. Their remarkable properties have established NDs as an invaluable regenerative medicine platform, with a broad range of clinically relevant applications ranging from targeted delivery systems for insoluble drugs, bioactive substrates for stem cells, and fluorescent probes for long-term tracking of cells and biomolecules in vitro and in vivo. This review introduces the synthesis techniques and the various routes of surface functionalization that allow for precise control over the properties of NDs. It also provides an in-depth overview of the current progress made towards the use of NDs in the fields of drug delivery, tissue engineering, and bioimaging. Their future outlook in regenerative medicine including the current clinical significance of NDs, as well as the challenges that must be overcome to successfully translate the reviewed technologies from research platforms to clinical therapies will also be discussed.

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Section: Synthesis and Functionalization Of Ndsmentioning

confidence: 99%

Section: Delivery Of Therapeuticsmentioning

confidence: 99%

Multifunctional nanodiamonds in regenerative medicine: Recent advances and future directions

Whitlow

Pacelli

Paul

2017

Journal of Controlled Release

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“…In many of those applications, functional biological molecules need to be tailored to the ND surfaces. Even though many approaches to attach biological molecules to ND surfaces have been investigated [5,8,12,[14][15][16], it remains challenging to monitor behaviors of the grafted molecules because the presence of ND hampers the employment of standard techniques (e.g., chromatography and optical spectroscopy).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, in a majority of cases, NDs contain nitrogen-vacancy (NV) centers, which are atomicscale fluorescent defects with excellent quantum properties. Thus, ND is a promising material for applications of biological imaging, targeted drug delivery, and nano-scale sensing [1][2][3][4][5][6][7][8][9][10][11][12][13]. In many of those applications, functional biological molecules need to be tailored to the ND surfaces.…”

Section: Introductionmentioning

confidence: 99%

High-Frequency Electron Paramagnetic Resonance Spectroscopy of Nitroxide-Functionalized Nanodiamonds in Aqueous Solution

Akiel

Stepanov

Takahashi

2016

Cell Biochem Biophys

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Nanodiamond (ND) is an attractive class of nanomaterial for fluorescent labeling, magnetic sensing of biological molecules, and targeted drug delivery. Many of those applications require tethering of target biological molecules on the ND surface. Even though many approaches have been developed to attach macromolecules to the ND surface, it remains challenging to characterize dynamics of tethered molecule. Here, we show high-frequency electron paramagnetic resonance (HF EPR) spectroscopy of nitroxide-functionalized NDs. Nitroxide radical is a commonly used spin label to investigate dynamics of biological molecules. In the investigation, we developed a sample holder to overcome water absorption of HF microwave. Then, we demonstrated HF EPR spectroscopy of nitroxide-functionalized NDs in aqueous solution and showed clear spectral distinction of ND and nitroxide EPR signals. Moreover, through EPR spectral analysis, we investigate dynamics of nitroxide radicals on the ND surface. The demonstration sheds light on the use of HF EPR spectroscopy to investigate biological molecule-functionalized nanoparticles.

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“…140 The modified nanodiamonds are biocompatible and possess excellent chemical, mechanical, and photostability. 141,142 As described in the following sections in this review, click chemistry has been so widely used in a variety of areas (e.g., drug synthesis, biology, molecular biology, materials science, and others), and nowadays is regarded as an indispensible tool for science and technology (of course, for nanoarchitectonics).…”

Section: Dna Nanoarchitectonicsmentioning

confidence: 99%

Chemistry Can Make Strict and Fuzzy Controls for Bio-Systems: DNA Nanoarchitectonics and Cell-Macromolecular Nanoarchitectonics

Komiyama

Yoshimoto

Sisido

et al. 2017

Bulletin of the Chemical Society of Japan

275

131

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In this review, we introduce two kinds of bio-related nanoarchitectonics, DNA nanoarchitectonics and cellmacromolecular nanoarchitectonics, both of which are basically controlled by chemical strategies. The former DNA-based approach would represent the precise nature of the nanoarchitectonics based on the strict or "digital" molecular recognition between nucleic bases. This part includes functionalization of single DNAs by chemical means, modification of the main-chain or side-chain bases to achieve stronger DNA binding, DNA aptamers and DNAzymes. It also includes programmable assemblies of DNAs (DNA Origami) and their applications for delivery of drugs to target sites in vivo, sensing in vivo, and selective labeling of biomaterials in cells and in animals. In contrast to the digital molecular recognition between nucleic bases, cell membrane assemblies and their interaction with macromolecules are achieved through rather generic and "analog" interactions such as hydrophobic effects and electrostatic forces. This cell-macromolecular nanoarchitectonics is discussed in the latter part of this review. This part includes bottom-up and top-down approaches for constructing highly organized cell-architectures with macromolecules, for regulating cell adhesion pattern and their functions in twodimension, for generating three-dimensional cell architectures on micro-patterned surfaces, and for building synthetic/natural macromolecular modified hybrid biointerfaces.

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Polyglycerol-functionalized nanodiamond as a platform for gene delivery: Derivatization, characterization, and hybridization with DNA

Cited by 53 publications

References 22 publications

Multifunctional nanodiamonds in regenerative medicine: Recent advances and future directions

Multifunctional nanodiamonds in regenerative medicine: Recent advances and future directions

High-Frequency Electron Paramagnetic Resonance Spectroscopy of Nitroxide-Functionalized Nanodiamonds in Aqueous Solution

Chemistry Can Make Strict and Fuzzy Controls for Bio-Systems: DNA Nanoarchitectonics and Cell-Macromolecular Nanoarchitectonics

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