Polygalacturonase secreted by yeasts from Brazilian semi-arid environments

Oliveira, R.; Rosa, Carlos A.; Uetanabaro, Ana Paula Trovatti; Azeredo, Antônio; Góes-Neto, Aristóteles; Assis, Sandra Aparecida de

doi:10.1080/09637480802534517

Cited by 12 publications

(8 citation statements)

References 27 publications

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“…After streaking 48 h-old yeast cultures onto the surface of the medium, the plates were incubated at 28ºC for five days and then revealed by the addition of a solution of hexadecyltrimethylammonium bromide (1 g/L), according to the procedure described by Oliveira et al (2009). Both activities were evident from the presence of a clear halo around the colonies.…”

Section: Pectinase and Polygalacturonase Activitiesmentioning

confidence: 99%

Characterisation of Hanseniaspora Isolates with Potential Aroma-enhancing Properties in Muscat Wines

López

Mateo

Maicas

2016

SAJEV

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The sensorial characteristics of the wines produced with Muscat grapes are related to the level of terpene alcohols, so an improvement of such a level is expected as a result of hydrolytic processes conducted by Hanseniaspora. The aim of this work was to select and identify new strains for this purpose. In a second step, the strains were assayed to evaluate their oenological abilities. H. uvarum H107 and H. vineae G26 and P38 were proven to be good candidates to be used in commercial vinification processes to enhance wine properties. Wine inoculated with yeasts showed an increase in the level of aromatic compounds.

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Section: Pectinase and Polygalacturonase Activitiesmentioning

confidence: 99%

Characterisation of Hanseniaspora Isolates with Potential Aroma-enhancing Properties in Muscat Wines

López

Mateo

Maicas

2016

SAJEV

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“…Pectin degrading activity was identified in Moesziomyces antarcticus , Kalmonozyma fusiformata and Pseudozyma sp. [ [129] , [130] , [131] ].…”

Section: Microbial Strain Engineering Of Filamentous Fungi Yeast Andmentioning

confidence: 99%

Innovations in CAZyme gene diversity and its modification for biorefinery applications

Chettri

Verma

2020

Biotechnology Reports

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Highlights The review analyses the role of CAZymes in lignocellulose degradation with focus on the different approaches for improving the enzyme activity. Omics based approaches for the analysis of genes, transcripts, proteins and metabolites related to plant degrading mechanism. Directed evolution and rational design as approaches for engineering to enhancing the CAZyme properties. Microbial engineering of the fungal and microbial strains by adapting techniques like CRISPR/cas9 and use of various expression systems. Novel enzymes and cocktail formulation for biorefinery-based application for production of value-added products using low cost plant biomass.

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“…collected in natural environments of the Brazilian semi-arid region, Bahia, Brazil and obtained at the Microorganisms Culture Collection of Bahia (CCMB) -UEFS, Brazil. Molecular identification was performed according to Oliveira et al, (2009).…”

Section: Microorganismmentioning

confidence: 99%

Extraction and characterization of β-fructofuranosidases produced by Kluyveromyces marxianus CCMB 322

Peixoto

Cedro

Mendes

et al. 2020

RSD

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Invertase (β-fructofuranosidase, EC 3.2.1.26) catalyzes sucrose hydrolysis into glucose and fructose and it is one of the simplest carbohydrases. These enzymes occur widely in nature and their presence has been reported in microorganisms and plants. Since yeasts are the main industrial source, most researches concerning this enzyme have focused on invertase extracted from such source. This study extracted and characterized inverted intracellular (Inv-I) and extracellular (Inv-E) of Kluyveromyces marxianus CCMB 322 isolated in the baiano semi-arid region. Kluyveromyces marxianus CCMB 322 produces intracellular and extracellular invertase with different characteristics. The optimum activity was achieved at approximately pH 3.9 and 45ºC, in Inv-I and Inv-E. The invertases produced by K. marxianus CCMB 322 showed thermal stability similar to that found in other studies. The Km and Vmax values of the Inv-I enzyme were 61.12mM and 5.56 µmol/mL.min-1, but the Km and Vmax values of the Inv-E enzyme were 76.5mM and 0.364 µmol/mL.min-1. Inverted from K. marxianus is a higher affinity for sucrose compared to enzymes from other sources.

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Polygalacturonase secreted by yeasts from Brazilian semi-arid environments

Cited by 12 publications

References 27 publications

Characterisation of Hanseniaspora Isolates with Potential Aroma-enhancing Properties in Muscat Wines

Characterisation of Hanseniaspora Isolates with Potential Aroma-enhancing Properties in Muscat Wines

Innovations in CAZyme gene diversity and its modification for biorefinery applications

Extraction and characterization of β-fructofuranosidases produced by Kluyveromyces marxianus CCMB 322

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