Polyethylenimine linked with chitosan improves astaxanthin production in Haematococcus pluvialis

Lee, Jae Been; Park, Yun Hwan; Jeon, Min Seo; Kim, Sok; Choi, Young Joon

doi:10.1007/s00253-022-12275-5

Cited by 5 publications

(3 citation statements)

References 51 publications

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“…under those conditions. As was shown previously [42,43], immobilization of cells on PEI-based carriers is stressful per se: thus, it increased accumulation of atsaxanthin in Haematococcus pluvialis in comparison with its suspended culture. Likewise, immobilization of the Lobosphaera sp.…”

Section: Discussionsupporting

confidence: 60%

Immobilization on Polyethylenimine and Chitosan Sorbents Modulates the Production of Valuable Fatty Acids by the Chlorophyte Lobosphaera sp. IPPAS C-2047

Vasilieva¹,

Shibzukhova²,

Solovchenko³

et al. 2023

Preprint

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Green microalgae including those from the genus Lobosphaera are exploited in biotechnology to obtain valuable fatty acids e.g., arachidonic acid (C20:4, ARA) for the production of infant formulae, food and feed additives. In nature, microalgae frequently exist in naturally immobilized state (as biofilms) with limited cell division rate and increased stress resilience. In biotechnology, immobilization of microalgae on artificial cell carriers simplifies biomass harvesting, increases culture robustness and productivity. The choice of suitable cell carrier is central to biotechnology of immobilized culture. Cell carriers based on the natural amine-containing polymer chitosan and synthetic polyethylenimine (PEI) are promising candidates for immobilization of phototrophic microorganisms. This is the first report on the effects of immobilization on PEI and chitosan on the accumulation and composition of polyunsaturated fatty acids, including ARA, in Lobosphaera sp. IPPAS C-2047. The immobilization on PEI increased ARA percentage in the total tatty acids and ARA accumulation by 72% and 81%, compared with the suspended cells cultured respectively in complete or nitrogen-deprived medium 14 days. Immobilization of Lobosphaera sp. on the chitosan-based carrier declined ARA but increased oleic and α-linoleic acid percentages. Mechanisms of the effects of immobilization on fatty acid profiles of the microalgae are discussed.

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Section: Discussionsupporting

confidence: 60%

Immobilization on Polyethylenimine and Chitosan Sorbents Modulates the Production of Valuable Fatty Acids by the Chlorophyte Lobosphaera sp. IPPAS C-2047

Vasilieva¹,

Shibzukhova²,

Solovchenko³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…As has been shown previously [41,42], immobilization of cells on PEI-based carriers is itself stressful: thus, it increased the accumulation of atsaxanthin in Haematococcus pluvialis in comparison to its suspended culture. Likewise, immobilization of Lobosphaera sp.…”

Section: Discussionmentioning

confidence: 54%

Immobilization on Polyethylenimine and Chitosan Sorbents Modulates the Production of Valuable Fatty Acids by the Chlorophyte Lobosphaera sp. IPPAS C-2047

Vasilieva

Shibzukhova

Solovchenko

et al. 2023

JMSE

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Green microalgae, including those from the genus Lobosphaera, are exploited in various fields of biotechnology to obtain valuable fatty acids (e.g., arachidonic acid (C20:4, ARA)) for the production of infant formulae, food and feed additives. In nature, microalgae frequently exist in naturally immobilized state (as biofilms) with a limited cell division rate and increased stress resilience. In the fields of biotechnology, immobilization of microalgae on artificial cell carriers simplifies biomass harvesting and increases culture robustness and productivity. The choice of a suitable cell carrier is central to biotechnology involving immobilized cultures. Cell carriers based on the natural amine-containing polymer chitosan and synthetic polyethylenimine (PEI) are promising candidates for immobilization of phototrophic microorganisms. This is the first report on the effects of immobilization on PEI and chitosan on the accumulation and composition of polyunsaturated fatty acids, including ARA, in Lobosphaera sp. IPPAS C-2047. Immobilization on PEI increased the ARA percentage in the total fatty acids and ARA accumulation by 72% and 81% compared to the suspended cells cultured in complete or nitrogen-deprived medium 14 days, respectively. Immobilization of Lobosphaera sp. on the chitosan-based carrier reduced the ARA percentage but increased oleic and α-linoleic acid percentages. The mechanisms of the effects of immobilization on the fatty acid profiles of the microalgae are discussed.

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“…The production efficiency of astaxanthin can reach more than 90%. In addition, polyvinylimine and chitosan can also be reused [22] . It was found that under the induction of succinic acid, endogenous hydrogen sulfide improved astaxanthin production by mediating related gene transcription levels and reactive oxygen signaling.…”

Section: Algaementioning

confidence: 99%

Advances in Astaxanthin Biosynthesis

2024

AJALS

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Astaxanthin is a fat-soluble and water-soluble pigment, a red carotenoid widely found in oysters, rhodococcus, and salmon. It is a powerful natural antioxidant that can effectively eliminate free radicals in the body, combat aging, clear vascular waste, reduce fatigue, regulate immunity, and exhibit a variety of biological activities. It is widely used in medicine, food, and cosmetics. Although natural astaxanthin is present in a variety of organisms, its content is very low, the extraction process is complex, the yield is small, and the price is expensive. With the identification of key genes for astaxanthin synthesis, it is possible to construct the astaxanthin biosynthesis pathway in microorganisms, synthesize the precursor substances of astaxanthin, or directly synthesize astaxanthin. Microbial synthesis of astaxanthin is environmentally friendly, cost-effective, involves small microbial size, rapid growth, strong vitality, and has garnered significant attention in the industrial production of astaxanthin. This paper reviews the recent advancements in the synthesis of astaxanthin by algae, fungi, and bacteria, as well as the efforts to increase its production by microorganisms.

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Polyethylenimine linked with chitosan improves astaxanthin production in Haematococcus pluvialis

Cited by 5 publications

References 51 publications

Immobilization on Polyethylenimine and Chitosan Sorbents Modulates the Production of Valuable Fatty Acids by the Chlorophyte Lobosphaera sp. IPPAS C-2047

Immobilization on Polyethylenimine and Chitosan Sorbents Modulates the Production of Valuable Fatty Acids by the Chlorophyte Lobosphaera sp. IPPAS C-2047

Immobilization on Polyethylenimine and Chitosan Sorbents Modulates the Production of Valuable Fatty Acids by the Chlorophyte Lobosphaera sp. IPPAS C-2047

Advances in Astaxanthin Biosynthesis

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