Polyethyleneterephthalate Provides Superior Retention of Endothelial Cells During Shear Stress Compared to Polytetrafluoroethylene and Pericardium

Capability of human umbilical cord blood progenitor-derived endothelial cells to form an efficient lining on a polyester vascular graft in vitro

“…Fluid shear stress exerts many effects on vascular ECs and plays an important role in maintaining the homeostasis of blood vessels. Consistent with Wong et al's study [47] and that of Sreerekha et al [51], our results exhibit an effective cell retention on polyethylene terephthalate. In addition, it was shown in 1997 [52] that confluent cultures of ECs without application of hemodynamic forces undergo morphological changes that indicate apoptotic cell death, which can be prevented by exposure to laminar flow.…”

“…From our results, endothelial attachment appears to be related to CSD, with the absolute number of attached cells on patches being nearly fivefold higher at 24 h for CSD2 compared with CSD1. Although seeding densities ranging from 8 Â 10 4 to 1.5 Â 10 6 cm À2 have been reported [17,36,[45][46][47], plating efficiencies have rarely been detailed in the literature. Anderson et al [45] established that cell attachment was directly related to the number of cells available in the cell suspension medium, with a saturation cell density of approximately 3.5 Â 10 5 cm À2 in the case of polytetrafluoroethylene grafts precoated with type I collagen.…”

“…Anderson et al [45] established that cell attachment was directly related to the number of cells available in the cell suspension medium, with a saturation cell density of approximately 3.5 Â 10 5 cm À2 in the case of polytetrafluoroethylene grafts precoated with type I collagen. There is no consensus on the optimal seeding density [47].…”

Bio- And Hemocompatible Tissue-Engineered Polycaprolactone Scaffolds Seeded by Human Endothelial and Smooth Muscle Cells After Implantation in the Abdominal Aorta of Scid Mice

“…В качестве каркаса для ТИС используют раз-личные материалы -как биологические [2,4], так и синтетические [3,5,6]. В нашей предыду-щей статье [11] мы сравнили разные виды мате-риалов и показали, что синтетические каркасы, полученные методом электроспиннинга, обе-спечивают более удовлетворительное заселение клетками, их адгезию и пролиферацию в отли-чие от ксеноперикарда [18,19], децеллюляризи-рованной гомоартерии [20,21,22], ПТФЭ и ПЭТ [23,24,25,7].…”

Evaluation of the Functional Properties of Human Endothelial and Smooth Muscle Cells After Seeding on the Surface of Natural and Synthetic Materials

“…Однако неудов-летворительное заселение клетками ограничивает использование этого материала для дальнейшего исследования. Слабое заселение перикарда клетка-ми подтверждают и другие исследования [16,17], в которых отмечено, что после 16 часов заселенные клетки начинают постепенно терять свои виталь-ные функции.…”