Polyethylene glycol-mediated blocking and monolayer morphology of an electrochemical aptasensor for malaria biomarker detection in human serum

Figueroa-Miranda, Gabriela; Wu, Changtong; Zhang, Yuting; Nörbel, Lena; Lo, Yvonne; Tanner, Julian A.; Elling, Lothar; Offenhäusser, Andreas; Mayer, Dirk

doi:10.1016/j.bioelechem.2020.107589

Cited by 34 publications

(25 citation statements)

References 64 publications

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“…5 A). Furthermore, thiol terminated PEG molecules were used as anti-fouling backfill to suppress unspecific binding [ 78 ]. The binding of the target to the receptor layer induced conformational changes within the aptamer film.…”

Section: Resultsmentioning

confidence: 99%

DNA aptamer selection for SARS-CoV-2 spike glycoprotein detection

Martínez-Roque

Franco-Urquijo

García-Velásquez

et al. 2022

Analytical Biochemistry

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Section: Resultsmentioning

confidence: 99%

DNA aptamer selection for SARS-CoV-2 spike glycoprotein detection

Martínez-Roque

Franco-Urquijo

García-Velásquez

et al. 2022

Analytical Biochemistry

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“…We and others observed that SAMs with molecular structural variations in charges proved to greatly impact their wettability and antifouling properties. 27,30,31 We anticipate that such ability of SAMs to modulate these surface properties would, in retrospect, provide a means to tune the performance of E-AB sensors, such as baseline stability, sensitivity, specificity, etc. As our proof-of-concept study, we incorporated here a DOX-detecting aptamer into an E-AB sensor platform and fabricated a series of sensors from six SAMs.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Exploring End-Group Effect of Alkanethiol Self-Assembled Monolayers on Electrochemical Aptamer-Based Sensors in Biological Fluids

Wang

Zhang

et al. 2021

Anal. Chem.

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The continuous, real-time monitoring of specific analytes in situ in biological fluids would provide personalized, high-precision pharmacokinetic information for the goal of precision medicine. Due to their conformationally linked signaling mechanism, electrochemical aptamer-based (E-AB) sensors are promising candidates for accurate measurements in such complex media. They suffer, however, from severe baseline drift when interrogated continuously and in real-time manner. In response, here, we investigate a couple of self-assembled monolayers in the application of E-AB sensors, achieving the improvement of their baseline stability and simultaneous modulation of sensor performance, e.g., target affinity and specificity.

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“…Nevertheless, still some challenges in the LOD and the requirement of complex microfluidic systems strongly limit their practicality. Very low LODs are achieved by electrochemical biosensors, but they only work with serum [25,26] or serial dilutions are needed if a quantitative measurement is required [27]. Fluorescence-based biosensors show a great potential for point-of-care applications since the sensitivity can be drastically increased by using plasmon nanostructures as a fluorescence enhancer and the sample pretreatment can be avoided [12].…”

Section: Introductionmentioning

confidence: 99%

Randomly positioned gold nanoparticles as fluorescence enhancers in apta-immunosensor for malaria test

et al. 2021

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A plasmon-enhanced fluorescence-based antibody-aptamer biosensor — consisting of gold nanoparticles randomly immobilized onto a glass substrate via electrostatic self-assembly — is described for specific detection of proteins in whole blood. Analyte recognition is realized through a sandwich scheme with a capture bioreceptor layer of antibodies — covalently immobilized onto the gold nanoparticle surface in upright orientation and close-packed configuration by photochemical immobilization technique (PIT) — and a top bioreceptor layer of fluorescently labelled aptamers. Such a sandwich configuration warrants not only extremely high specificity, but also an ideal fluorophore-nanostructure distance (approximately 10–15 nm) for achieving strong fluorescence amplification. For a specific application, we tested the biosensor performance in a case study for the detection of malaria-related marker Plasmodium falciparum lactate dehydrogenase (PfLDH). The proposed biosensor can specifically detect PfLDH in spiked whole blood down to 10 pM (0.3 ng/mL) without any sample pretreatment. The combination of simple and scalable fabrication, potentially high-throughput analysis, and excellent sensing performance provides a new approach to biosensing with significant advantages compared to conventional fluorescence immunoassays. Graphical abstract

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Polyethylene glycol-mediated blocking and monolayer morphology of an electrochemical aptasensor for malaria biomarker detection in human serum

Cited by 34 publications

References 64 publications

DNA aptamer selection for SARS-CoV-2 spike glycoprotein detection

DNA aptamer selection for SARS-CoV-2 spike glycoprotein detection

Exploring End-Group Effect of Alkanethiol Self-Assembled Monolayers on Electrochemical Aptamer-Based Sensors in Biological Fluids

Randomly positioned gold nanoparticles as fluorescence enhancers in apta-immunosensor for malaria test

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