Polyelectrolyte multilayer films functionalized with peptides for promoting osteoblast functions

Tsai, Wei‐Bor; Chen, Rita P.‐Y.; Wei, Kuang-Ling; Chen, Yi-Ru; Liao, Tai-Yan; Liu, Hsuan‐Liang; Lai, Juin‐Yih

doi:10.1016/j.actbio.2009.05.034

Cited by 57 publications

(51 citation statements)

References 42 publications

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“…98 To demonstrate the efficacy of PEMs functionalized with short peptide sequences, Tsai et al constructed PAH/ PAA PEMs comprised of five bilayers, with a terminal RGD-PAH layer. 99 In this study, the incorporation of RGD stimulated osteoblast adhesion, proliferation, and phenotype. 99 The assembly of the multilayer was conducted at pH of 2 and 6.5.…”

Section: Detzel Et Almentioning

confidence: 99%

“…99 In this study, the incorporation of RGD stimulated osteoblast adhesion, proliferation, and phenotype. 99 The assembly of the multilayer was conducted at pH of 2 and 6.5. At lower pH, the anionic PE was incorporated at increased nonstoichiometric ratios to compensate for the fully ionized cationic PE (*70% PAA, 30% PAH at pH 2.0).…”

Section: Detzel Et Almentioning

confidence: 99%

“…At lower pH, the anionic PE was incorporated at increased nonstoichiometric ratios to compensate for the fully ionized cationic PE (*70% PAA, 30% PAH at pH 2.0). 99,100 Consequently, when the terminal RGD-PAH layer was deposited, the concentration of RGD molecules at the surface increased fivefold at pH 2.0. 99 Despite the increased RGD surface concentration, osteoblast adhesion and proliferation were unaffected since the concentration of RGD exceeded 0.6 pmol/ cm 2 .…”

Section: Detzel Et Almentioning

confidence: 99%

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Polyelectrolyte Multilayers in Tissue Engineering

Detzel

Larkin

Rajagopalan

2011

Tissue Engineering Part B: Reviews

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The layer-by-layer assembly of sequentially adsorbed, alternating polyelectrolytes has become increasingly important over the past two decades. The ease and versatility in assembling polyelectrolyte multilayers (PEMs) has resulted in numerous wide ranging applications of these materials. More recently, PEMs are being used in biological applications ranging from biomaterials, tissue engineering, regenerative medicine, and drug delivery. The ability to manipulate the chemical, physical, surface, and topographical properties of these multilayer architectures by simply changing the pH, ionic strength, thickness, and postassembly modifications render them highly suitable to probe the effects of external stimuli on cellular responsiveness. In the field of regenerative medicine, the ability to sequester growth factors and to tether peptides to PEMs has been exploited to direct the lineage of progenitor cells and to subsequently maintain a desired phenotype. Additional novel applications include the use of PEMs in the assembly of three-dimensional layered architectures and as coatings for individual cells to deliver tunable payloads of drugs or bioactive molecules. This review focuses on literature related to the modulation of chemical and physical properties of PEMs for tissue engineering applications and recent research efforts in maintaining and directing cellular phenotype in stem cell differentiation.

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Section: Detzel Et Almentioning

confidence: 99%

Section: Detzel Et Almentioning

confidence: 99%

Section: Detzel Et Almentioning

confidence: 99%

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Polyelectrolyte Multilayers in Tissue Engineering

Detzel

Larkin

Rajagopalan

2011

Tissue Engineering Part B: Reviews

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“…These peptides are ligands for some of the integrin family cell-surface receptors, which are an important class of receptors found on all cellular surfaces and that mediate attachment of cells to the extracellular matrix (Critchley (2000)). Many different peptides fragments have been used over the years to promote cell adhesion to electrode surfaces or chip surfaces (Huang et al (2009);Tsai et al (2009); Van Meerbergen et al (2008)). …”

Section: Gy Vtgpfu Cpf Ejcnngpigu Kp Vjg Fgxgnqrogpv Qh Oketqhcdtkecmentioning

confidence: 99%

New Trends and Challenges in the Development of Microfabricated Probes for Recording and Stimulating of Excitable Cells

Braeken¹,

Prodanov²

2010

New Developments in Biomedical Engineering

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types of neural prostheses are unclear. Notable exceptions for this are the auditory cochlear prostheses, which exploit the sonotopic organization of the cochlea, the cardiac pacemakers, the phrenic stimulator and the foot drop stimulator. Current DBS systems for Parkinson's disease rely on the high frequency stimulation, which can result in blocking of certain mid-brain pathways. The encoding of the kinematic information in these structures is still unknown. Dissociated cell cultures lose their anatomical connectivity and structure when seeded on substrates in vitro. The 'new' situation that exists on these surfaces is interesting in terms of communication between individual cells and their function in advanced networks. However, the translation of these processes to the in vivo situation is not always straightforward and challenging. On the level of the overall device several types of issues can be grouped in the categories of configuration and operation. Issues related to the configuration can be related to the assembly, the size and topology of the device, the encapsulation and the packaging of the device. Devices have to be packaged in a way that prevents leakage of the environment, which contains high salt and protein concentrations, to the chip. These packages have to be leakage-proof on the one hand and non-toxic for the cell culture or tissue on the other hand. Issues related to the operation of the device can be grouped under the power, safety and communication of the device. The operation has to be compatible with cell cultures or in vivo environments, which in this case has to be in agreement with certain regulations. One other important aspect of the safety of operation are the interactions with other equipment, for example MRI.

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“…На матрице иммобилизуют также биоактивные пептиды (например, RGD пептид), которые взаимодействуют с мембранными рецепторами клетки и обеспечивают более хорошую адгезию и распластывание клеток на полимерной матрице [23].…”

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Delta-sleep inducing peptide entrapment and release from polymer hydrogels based on modified polyvinyl alcohol

et al. 2013

BIOMED KHIM

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1Институт биоорганической химии имени академиков М.М. Шемякина и Ю.А. Овчинникова, 117997, Москва, ул. Миклухо-Маклая, 16/10; тел.: (495)3360600; эл. почта: sukhanovat@mail.ru 2 Российский химико-технологический университет имени Д.И. Менделеева, МоскваЦелью работы было включение дельта-сон индуцирующего пептида (ДСИП) в сшитые полимерные гидрогели на основе поливинилового спирта различного строения с последующим изучением кинетики выхода из них пептида в модели in vitro.В работе были использованы изотропные и макропористые гидрогели на основе акрилового производного поливинилового спирта (Акр-ПВС), а также эпоксидсодержащие макропористые гидрогели, получаемые сополимеризацией данного макромера с глицидиловым эфиром метакриловой кислоты В макропористый гидрогель ПВС пептид включали путем нанесения его водного раствора на предварительно сформированную матрицу, в эпоксидсодержащий гель ПВС-ГМА -путём адсорбции из водного раствора, а в изотропный гель ПВС -путём введения пептида в полимерную смесь в процессе формирования гидрогеля. Кинетику выхода пептида из гидрогелей исследовали при инкубации матриц в фосфатно-солевом буфере (PBS; pH 7,4), в физ. растворе (0,9% раствор NaCl) и в воде, определяя концентрацию ДСИП в супернатантах с помощью обращённо-фазовой ВЭЖХ.Из макропористого гидрогеля ПВС в течение первых 30 мин инкубации выход ДСИП составлял 74%, 70% и 64% в воде, PBS и 0,9% растворе NaCl, соответственно, а завершался через 3 ч. Из гидрогеля, содержавшего эпоксигруппы, даже спустя 48 ч инкубации не наблюдали выделения ни пептида, ни продуктов его распада.Для свежеприготовленного изотропного геля кинетика выхода пептида выглядела следующим образом: 27% в течение первых 30 мин и 78% через 33 ч. Для лиофильно высушенных образцов гидрогелей выход пептида составлял 63% в течение первых 30 мин, в то время как высушивание образцов гидрогеля при комнатной температуре в течение 3 сут. приводило к потере значительной части пептида в результате разрушения его структуры.Ключевые слова: макропористые полимерные гидрогели, модифицированный ПВС, дельта-сон индуцирующий пептид, включение пептидов, кинетика выхода пептида in vitro, тканевая инженерия. 65 * -адресат для переписки

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Polyelectrolyte multilayer films functionalized with peptides for promoting osteoblast functions

Cited by 57 publications

References 42 publications

Polyelectrolyte Multilayers in Tissue Engineering

Polyelectrolyte Multilayers in Tissue Engineering

New Trends and Challenges in the Development of Microfabricated Probes for Recording and Stimulating of Excitable Cells

Delta-sleep inducing peptide entrapment and release from polymer hydrogels based on modified polyvinyl alcohol

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