Polyelectrolyte‐modified short microchannel for cation separation

Bai, Xiaoxia; Roussel, Christophe; Jensen, Henrik

doi:10.1002/elps.200305771

Cited by 28 publications

(21 citation statements)

References 32 publications

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“…3 Ratio hydroquinones 1a-d/␤-lactoglobulin A 3g=: 5 mM/5 M, from [68]. 4 The tagging yield was found to increase from 27 to 52% in 80 minutes of infusion. mass spectrum, the number of which corresponds exactly to the number of cysteines in the analyzed peptides only with 2-carboxymethylhydroquinone 1d [69,87].…”

Section: On-line Tagging Of Peptides and Application To Protein Identmentioning

confidence: 98%

“…For example, analytical separations such as capillary electrophoresis (CE) [3,4], two-dimensional electrokinetic separations [5], liquid chromatography (LC) [6], reaction and detection microchambers [7][8][9] have been implemented in the microfluidic format. More recently, analytical microfluidic systems have caught interest in the particular field of proteomics [10,11] because of the high analytical constraints demanded by this field, namely the low amount of sample usually available, the high complexity of the initial protein mixture, and the need for high-throughput analysis [12].…”

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confidence: 99%

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Generation of mass tags by the inherent electrochemistry of electrospray for protein mass spectrometry

Roussel

Dayon

Lion

et al. 2004

J. Am. Soc. Mass Spectrom.

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We present herein a review of our work on the on-line electrochemical generation of mass tags toward cysteine residues in peptides and proteins. Taking advantage of the inherent electrochemical nature of electrospray generated from a microfabricated microspray emitter, selective probes for cysteine were developed and tested for on-line nonquantitative mass tagging of peptides and proteins. The nonquantitative aspect of the covalent tagging thus allows direct counting of free cysteines in the mass spectrum of a biomolecule through additional adduct peaks. Several substituted hydroquinones were investigated in terms of electrochemical properties, and their usefulness for on-line mass tagging during microspray experiments were assessed with L-cysteine, peptides, and intact proteins. Complementarily, numerical simulations were performed to properly understand the respective roles of mass transport, kinetics of electrochemical-chemical reactions, and design of the microspray emitter in the mass tagging overall efficiency. Finally, the on-line electrochemical tagging of cysteine residues was applied to the analysis of tryptic peptides of purified model proteins for protein identification through peptide mass fingerprinting. (J Am Soc Mass Spectrom 2004, 15, 1767-1779

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Section: On-line Tagging Of Peptides and Application To Protein Identmentioning

confidence: 98%

mentioning

confidence: 99%

Generation of mass tags by the inherent electrochemistry of electrospray for protein mass spectrometry

Roussel

Dayon

Lion

et al. 2004

J. Am. Soc. Mass Spectrom.

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“…Furthermore, it appears that quantitation is not a trivial task in microchip CE because of linearity [162,163] or limited resolution [164][165][166] problems.…”

Section: Microchip Technologymentioning

confidence: 98%

Recent trends in CE of inorganic ions: From individual to multiple elemental species analysis

Timerbaev

2007

Electrophoresis

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The major methodological developments in CE related to inorganic analysis are overviewed. This is an update to a previous review article by the author (Timerbaev, A. R., Electrophoresis 2004, 25, 4008-4031) and it covers the review work and innovative research papers published between January 2004 and the first part of 2006. As was underlined in that review, a growing interest of analytical community in providing elemental speciation information found a sound response of the CE method developers. Presently, almost every second research paper in the field of interest deals with element species analysis, the use of inductively coupled plasma MS detection and biochemical applications being the topics of utmost research efforts. On the other hand, advances in general methodology traditionally centered on a CE system modernization for improvements in sensitivity and separation selectivity have attracted less attention over the review period. While there is no indication that inorganic ion applications would surpass by the developmental rate the more matured analysis of organic analytes, CE can now be seen as an analytical technique to be before long customary in a number of inorganic analysis arenas.

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“…Nowadays, various polymeric materials [16] are used such as PDMS [17,18], PMMA [19,20], polycarbonate [21], poly(ethylenterephthalate) [22,23], thermoset polyester [24,25], and polyester toner [26,27], Zeonix/ Zeonor [28,29] or thermoplastic olefin polymer of amorphous structure [7,30,31].…”

Section: Introductionmentioning

confidence: 99%

New analytical portable instrument for microchip electrophoresis with electrochemical detection

Fernández-la-Villa¹,

Pozo-Ayuso²,

Castaño‐Álvarez³

2010

Electrophoresis

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A new portable instrument that includes a high voltage power supply, a bipotentiostat, and a chip holder has been especially developed for using microchips electrophoresis with electrochemical detection. The main unit of the instrument has dimensions of 150 x 165 x 70 mm (wxdxh) and consists of a four-outputs high voltage power supply with a maximum voltage of +/-3 KV and an acquisition system with two channels for dual amperometric (DC or pulsed amperometric detection) detection. Electrochemical detection has been selected as signal transduction method because it is relatively easily implemented, since nonoptical elements are required. The system uses a lithium-ion polymer battery and it is controlled from a desktop or laptop PC with a graphical user interface based on LabVIEW connected by serial RS232 or Bluetooth. The last part of the system consists of a reusable chip holder for housing the microchips, which contain all the electrical connections and reservoirs for making the work with microchips easy. The performance of the new instrument has been evaluated and compared with other commercially available apparatus using single- and dual-channel pyrex microchips for the separation of the neurotransmitters dopamine, epinephrine, and 3,4-dihydroxy-L-phenyl-alanine. The reduction of the size of the instrument has not affected the good performance of the separation and detection using microchips electrophoresis with electrochemical detection. Moreover, the new portable instrument paves the way for in situ analysis making the use of microchips electrophoresis easier.

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Polyelectrolyte‐modified short microchannel for cation separation

Cited by 28 publications

References 32 publications

Generation of mass tags by the inherent electrochemistry of electrospray for protein mass spectrometry

Generation of mass tags by the inherent electrochemistry of electrospray for protein mass spectrometry

Recent trends in CE of inorganic ions: From individual to multiple elemental species analysis

New analytical portable instrument for microchip electrophoresis with electrochemical detection

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