Polydopamine coating of uncrosslinked chitosan as an acellular scaffold for full thickness skin grafts

Bock, Nathalie; Pham, Truc Le Buu; Nguyen, Tran B.; Bình, Nguyễn Trọng; Tran, Hien A.; Tran, Phong A.

doi:10.1016/j.carbpol.2020.116524

Cited by 20 publications

(21 citation statements)

References 63 publications

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“…42 Films using a combined PDA-chitosan strategy have previously been demonstrated in the literature for skin grafts and bone tissue engineering. 37,38 Polydopamine conferred favorable changes in mechanical properties and promoted cell adhesion and proliferation. However, there is a lack of full characterization including both physical, mechanical, and chemical analysis as well as cell culture work in relation to PDA coating time, which is discussed below.…”

Section: Resultsmentioning

confidence: 99%

“…Previous findings stated that PDA coating improves cell viability, metabolic activity, and leads to flatter morphology compared to plain chitosan. 37,49 As noted above, while plain chitosan surfaces are hydrophobic, polydopamine-modified chitosan films are hydrophilic, which help in increased cell adhesion through non-specific binding compared to plain chitosan. 38,49 Additionally, the organic functional groups and molecular chains from the polydopamine layer provide more binding sites for cell-adhesive proteins such as fibronectin and vitronectin, increasing cell adhesion and spreading.…”

Section: Cell Culturementioning

confidence: 97%

“…Our observations for plain chitosan is in agreement with prior work showing the tensile strength of dry chitosan films of thickness 0.01-0.04 mm is in the realm of 40-60 MPa depending on solution casting concentration, and has about 1%-5% strain at break. 41,45 However, Bock et al 37 reported changes in the tensile properties of wet chitosan and PDAchitosan films, stating that coating did not significantly affect tensile strength, and elongation at break was slightly lower for PDA-coated substrates. While the actual values for wet and dry films differ, no significant trend in tensile strength was observed, while the elongation at break increased for PDA-coated films.…”

Section: Mechanical Propertiesmentioning

confidence: 99%

“…These results were quantitatively and qualitatively consistent with those earlier seen for PDAchitosan films. 37,38 In order to study the stability of these coatings on the chitosan films, the films were left in a petridish exposed to air for 2 weeks. Another set of films was left soaked in PBS buffer for the same period.…”

Section: Mechanical Propertiesmentioning

confidence: 99%

“…28 While polydopaminechitosan hydrogels and nanoparticles have been previously studied, 36 coatings of chitosan films have been relatively unexplored. Bock et al 37 reported on PDA coating as an acellular scaffold for skin grafts. They showed that the coating improved hydrophilicity, with a significant increase in attachment and metabolic activity of mammalian cells in vitro.…”

Section: Introductionmentioning

confidence: 99%

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Facile fabrication and nanoscale assembly of polydopamine-functionalized, flexible chitosan films

Brooks

Imran

Pradhan

et al. 2021

Journal of Bioactive and Compatible Polymers

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Substrates that are simultaneously thin, strong, optically transparent, and biocompatible have diverse applications in a range of fundamental and applied fields. While nature-derived materials offer advantages of sustainability and inherent biocompatibility compared to synthetic polymers, their brittleness and swelling, as well as surface charge and chemical functionalization non-conducive to cell growth, can hinder widespread application. In this work, we discuss the fabrication and systematic characterization of polydopamine-coated chitosan thin films. Chitosan is a widely used, partially deacetylated form of chitin, derived from crustaceans and arthropods. Polydopamine (PDA) is derived from chemistries mimicking mussel foot adhesive proteins. A facile dip-coating process of thin and flexible, uncrosslinked chitosan films in aqueous dopamine solutions leads to dramatic changes in physical and chemical properties. We show how the PDA forms time-dependent assemblies on the film surfaces, affecting surface roughness, hydrophilicity, and mechanical strength. Coating the surface for even a few seconds provides functional changes to the films. Our results shows that the optimal coating time is on the order of few hours, whereby the films are optically transparent with excellent extensibility and Young’s modulus, while further coating reduces the benefits of this surface coating. These materials are biocompatible, serving as substrates for cell adhesion and growth while maintaining good viability. Overall, these findings give insight to the effects of PDA assembly on surfaces, and illustrate how a simple, quick, and robust bioinspired coating process can prime substrates for biomedical applications such as tissue engineering, biosensing, and wound healing.

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Section: Resultsmentioning

confidence: 99%

Section: Cell Culturementioning

confidence: 97%

Section: Mechanical Propertiesmentioning

confidence: 99%

Section: Mechanical Propertiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Facile fabrication and nanoscale assembly of polydopamine-functionalized, flexible chitosan films

Brooks

Imran

Pradhan

et al. 2021

Journal of Bioactive and Compatible Polymers

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Flexible Conductive Decellularized Fish Skin Matrix as a Functional Scaffold for Myocardial Infarction Repair

Fang

Zhan

et al. 2023

Macromolecular Bioscience

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Engineering cardiac patches are proven to be effective in myocardial infarction (MI) repair, but it is still a tricky problem in tissue engineering to construct a scaffold with good biocompatibility, suitable mechanical properties, and solid structure. Herein, decellularized fish skin matrix is utilized with good biocompatibility to prepare a flexible conductive cardiac patch through polymerization of polydopamine (PDA) and polypyrrole (PPy). Compared with single modification, the double modification strategy facilitated the efficiency of pyrrole polymerization, so that the patch conductivity is improved. According to the results of experiments in vivo and in vitro, the scaffold can promote the maturation and functionalization of cardiomyocytes (CMs). It can also reduce the inflammatory response, increase local microcirculation, and reconstruct the conductive microenvironment in infarcted myocardia, thus improving the cardiac function of MI rats. In addition, the excellent flexibility of the scaffold, which enables it to be implanted in vivo through “folding‐delivering‐re‐stretehing” pathway, provides the possibility of microoperation under endoscope, which avoids the secondary damage to myocardium by traditional thoracotomy for implantation surgery.This article is protected by copyright. All rights reserved

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PDA@Ti₃C₂T_x as a novel carrier for pesticide delivery and its application in plant protection: NIR‐responsive controlled release and sustained antipest activity

Wan

Fei

et al. 2021

Pest Management Science

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BACKGROUND Stimuli‐responsive pesticide controlled release system provides a new strategy for the development of high‐efficiency pesticides formulation. RESULTS In this article, we report a novel polydopamine surface modified MXene‐Ti3C2Tx nanocarrier for pesticide delivery and plant protection. Polydopamine modified Ti3C2Tx (PDA@Ti3C2Tx) nanocarrier was prepared by biomimetic self‐polymerization of dopamine on the surface of Ti3C2Tx. A typical pesticide, emamectin benzoate (EB), was loaded on PDA@Ti3C2Tx through physisorption process, with a high pesticide loading rate of 45.37%. PDA@Ti3C2Tx exhibited excellent photothermal conversion effect (η = 34.5%). Under the irradiation of near‐infrared (NIR) laser, EB would sustained release from PDA@Ti3C2Tx nanocarrier to surrounding medium. Compared with free EB, EB@PDA@Ti3C2Tx exhibited prolonged persistence period, which can keep antipest activity at 14 days post spraying. In addition, PDA@Ti3C2Tx nanocarrier and EB@PDA@Ti3C2Tx nanoformulation are of good safety, showing no side effect to the seed germination and seedling growth. CONCLUSION This research developed a novel nanocarrier for water‐insoluble pesticide delivery, realizing NIR‐responsive controlled release and sustained antipest activity.

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Polydopamine coating of uncrosslinked chitosan as an acellular scaffold for full thickness skin grafts

Cited by 20 publications

References 63 publications

Facile fabrication and nanoscale assembly of polydopamine-functionalized, flexible chitosan films

Facile fabrication and nanoscale assembly of polydopamine-functionalized, flexible chitosan films

Flexible Conductive Decellularized Fish Skin Matrix as a Functional Scaffold for Myocardial Infarction Repair

PDA@Ti₃C₂T_x as a novel carrier for pesticide delivery and its application in plant protection: NIR‐responsive controlled release and sustained antipest activity

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Polydopamine coating of uncrosslinked chitosan as an acellular scaffold for full thickness skin grafts

Cited by 20 publications

References 63 publications

Facile fabrication and nanoscale assembly of polydopamine-functionalized, flexible chitosan films

Facile fabrication and nanoscale assembly of polydopamine-functionalized, flexible chitosan films

Flexible Conductive Decellularized Fish Skin Matrix as a Functional Scaffold for Myocardial Infarction Repair

PDA@Ti3C2Tx as a novel carrier for pesticide delivery and its application in plant protection: NIR‐responsive controlled release and sustained antipest activity

Contact Info

Product

Resources

About

PDA@Ti₃C₂T_x as a novel carrier for pesticide delivery and its application in plant protection: NIR‐responsive controlled release and sustained antipest activity