Polydopamine-assisted versatile modification of a nucleic acid probe for intracellular microRNA imaging and enhanced photothermal therapy

Abstract

Polydopamine can assist the versatile modification of a nucleic acid probe for intracellular miRNA responsed fluorescence imaging and enhanced photothermal therapy.

Recent advances in living cell nucleic acid probes based on nanomaterials for early cancer diagnosis

“… [56 , 57] miRNA-21 breast cancer MCF-7 miRNA-21 is highly expressed in a variety of cancers and is related to promoting tumor cell proliferation and inhibiting apoptosis. [58] , [59] , [60] , [61] , [62] , [63] , [64] , [65] , [66] MDA-MB-231 bladder cancer Biu-87 cervical cancer HeLa hepatocellular cancer HepG2 lung cancer A549 malignant cancer A375 miRNA-155 DLBCL DLBCL Highly expressed in lung, renal, hepatocellular, colorectal and breast cancers et al Promote the stemness of cancer cells, increase drug resistance to chemotherapy and radiotherapy, and can be used for tumor immunotherapy. [20 , 27 , 67 , 68] breast cancer MCF-7 miRNA-1246 lung cancer A549 miRNA-1246 can exert oncogenic roles in various cancers.…”

Recent Advances in a Polydopamine-Mediated Antimicrobial Adhesion System

“…Hence, PDA coatings can play the role as anchor to tether biopolymers consisting of thiols and amines ( Lee et al, 2009 ; Huang et al, 2015 ; Liu and Huang, 2016 ; Tang et al, 2018 ; Yang et al, 2019 ). This has allowed immobilization of oligonucleotide-based aptamers and molecular probes and multifunctional nanoparticles for potential applications in nanomedicine and biosensors ( Wang C. et al, 2016 ; Zheng et al, 2018 ; Jia et al, 2019 ). The phenolic groups of PDA have been reported to enhance the nucleation sites in the carbon dot formation to fabricate fluorescent PDA-passivated N-doped carbon dots ( Bai et al, 2018 ).…”

Nucleic‐Acid Structures as Intracellular Probes for Live Cells

“…Furthermore, Zheng et al developed a NanoFlare‐like construct using PDA‐coated gold nanoparticles . Target binding to the recognition strand results in the fluorophore strand's displacement, leading to fluorescence turn‐on.…”

“…D) As opposed to chemical conjugation, fluorophore‐labeled ONTs may also be adsorbed onto surface‐coated gold nanoparticles. For example, as depicted in the figure, fluorophore‐tagged hairpin ONTs can be adsorbed onto polydopamine (colored black)‐coated gold nanoparticles . Polydopamine acts as a further source of quenching and can be utilized in photothermal therapy due its ability to absorb NIR light.…”