Polydatin inhibits growth of lung cancer cells by inducing apoptosis and causing cell cycle arrest

Abstract

Polydatin (PD), a small natural compound from Polygonum cuspidatum, has a number of biological functions. However, the anticancer activity of PD has been poorly investigated. In the present study, thiazolyl blue tetrazolium bromide assay was used to evaluate the inhibitory effect of PD on cell growth. Cell cycle distribution and apoptosis were investigated by flow cytometry. In addition, the expression of several proteins associated with apoptosis and cell cycle were analyzed by western blot analysis. The results demonstrated that PD significantly inhibits the proliferation of A549 and NCI-H1975 lung cancer cell lines and causes dose-dependent apoptosis. Cell cycle analysis revealed that PD induces S phase cell cycle arrest. Western blot analysis showed that the expression of Bcl-2 decreased as that of Bax increased, and the expression of cyclin D1 was also suppressed. The results suggest that PD has potential therapeutic applications in the treatment of lung cancer.

Polydatin-induced cell apoptosis and cell cycle arrest are potentiated by Janus kinase 2 inhibition in leukemia cells

“…Due to these properties, PD is more easily absorbed in the intestinal tract than resveratrol. PD has been shown to have cytotoxic effects on various cancer cell lines, including colon (13), lung (14) and nasopharyngeal (15) cancer cells; however, the underlying mechanism of action has largely remained elusive. The present study showed that PD, exerted anti-proliferative effects via inducing cell cycle arrest and induced mitochondria-mediated apoptosis in MOLT-4 leukemia cell lines, which was potentiated by a JAK2 inhibitor.…”

“…PD has been shown to have multiple beneficial therapeutic properties, including cardioprotective (7)(8)(9), hepatoprotective (10), neuroprotective (11) and anti-inflammatory (12) effects. PD has also been reported to have anti-cancer effects with activity against colon (13) and lung (14) cancer as well as nasopharyngeal carcinoma (15). However, to the best of our knowledge, the effects of PD on human leukemic cells have yet not been studied.…”

Polydatin Induces Apoptosis and Inhibits Growth of Acute Monocytic Leukemia Cells

“…Previous studies have demonstrated that PD has many bioactivities such as anti-platelet aggregation, against shock [4,9], anti-ischemia/reperfusion injury [7], hepato-protective effect [10,11], neuroprotective activity [12,13], lung protective effects [14], anti-inflammatory [15], immune-regulating functions [16], and anti-tumor activity [17,18]. But most of the anti-tumor studies are concerning solid tumors, little is known concerning the acute monocytic leukemia.…”

Molecular mechanisms of natural compounds in cell death induction and sensitization to chemotherapeutic drugs in lung cancer

“…Polydatin (PD; 3,4,5'‐trih ydroxystilbene‐3‐β‐D‐mono‐D‐glucoside), a natural compound derived from Polygonum cuspidatum root, is commonly used in traditional Chinese medicine (Wang, Song, Chen, Zhao, & Zhao, ), and PD treatment is known to show a reduction in antiapoptotic Bcl‐2 levels and an increase in proapoptotic Bax expression to initiate apoptosis in A549 human lung cancer cells (Zhang et al, ).…”