Polydatin, a Glycoside of Resveratrol, Induces Apoptosis and Inhibits Metastasis Oral Squamous Cell Carcinoma Cells In Vitro

Abstract

Although various methods, such as surgery and chemotherapy, are applied to the treatment of OSCC, there are problems, such as functional and aesthetic limitations of the mouth and face, drug side effects, and lymph node metastasis. Many researchers are making efforts to develop new therapeutic agents from plant-derived substances to overcome the side effects that occur in oral cancer treatment. Polydatin is known as a natural precursor of resveratrol, and research on its efficacy is being actively conducted recently. Therefore, we investigated whether polydatin can induce apoptosis and whether it affects cell migration and invasion through the regulation of EMT-related factors in OSCC. Polydatin decreased the survival and proliferation rates of CAL27 and Ca9-22 cells, and induced the release of cytochrome c, a factor related to apoptosis, and fragmentation of procaspase-3 and PARP. Another form of cell death, autophagy, was observed in polydatin-treated cells. In addition, polydatin inhibits cell migration and invasion, and it has been shown to occur through increased expression of E-cadherin, an EMT related factor, and decreased expression of N-cadherin and Slug and Snail proteins and genes. These findings suggest that polydatin is a potential oral cancer treatment.

Polydatin Attenuates Cisplatin-Induced Acute Kidney Injury via SIRT6-Mediated Autophagy Activation

“…et Zucc., is used for both medication and food, which plays a multitarget protective role in AKI by antiapoptosis, anti-inflammation, antioxidative stress, antiferroptosis, and regulation of autophagy [4][5][6][7][8][9][10][11][12]. What is more, PD has also been proved to play an antitumor effect by inhibiting cell viability, migration, and invasion of different tumor cells [13][14][15]. PD has been clinically used to treat multiple diseases, showing its safety in human application [16][17][18], which suggests that it can be used as a promising alternative compound for treating CP-AKI in cancer patients.…”

“…In recent years, the relationship between PD and autophagy has attracted considerable attention in different diseases, including sepsis [5,19,20], ischemia-reperfusion (I/R) injury [21][22][23], diabetes [24,25], atherosclerosis [26], Parkinson's disease [27], and tumors [13,[28][29][30]. PD has also been verified to play a nephroprotective role by regulating autophagy in sepsis-induced AKI [5,6] and diabetic nephropathy [25].…”

Chemopreventive and Anticancer Role of Resveratrol against Oral Squamous Cell Carcinoma

“…Additionally, the treatment with PD 0.25 mM increased the expression of ATG5 and LC3 proteins, thereby inducing autophagy in both Ca9-22 and Cal-27 cells. Finally, PD displayed antimetastatic properties due to the suppression of Snail and Slug proteins at the cell junction level, leading to the enhanced expression of E-cadherin and the downregulation of N-cadherin [ 45 ]. These results confirmed that the presence of the glycoside moiety did not alter the anticancer properties of RSV, thus confirming PD as a valid alternative to RSV in OSCC treatment and chemoprevention.…”

Polydatin: Pharmacological Mechanisms, Therapeutic Targets, Biological Activities, and Health Benefits

“…Furthermore, suppressing the phosphorylation of CREB and cyclin D1 gene expression might be associated with the apoptotic effect of polydatin on human breast cancer cells [ 101 ]. Moreover, extensive studies have demonstrated that the suppression of platelet-derived growth factor (PDGF)/Akt signaling [ 91 ], stimulation of cytochrome c release, and activation of poly (ADP-ribose) polymerase (PARP) fragmentation [ 109 ] could induce cell cycle arrest and apoptosis on polydatin-treated laryngeal/cervical cancer and oral squamous cell carcinoma.…”