Polycystic ovarian syndrome (PCOS) refers to the presence of small, benign and painless cysts in the ovaries, usually manifested clinically by a faction of symptoms and changes in hormonal levels. Despite the fact that its prevalence is global, the knowledge of its etiology and management is still grossly inadequate even among women who are affected in various parts of the world. The study therefore investigated the level of knowledge of women of reproductive age group about polycystic ovarian syndrome and its effect on the reproductive hormones. A sample of ninety-four (94) subjects, consisting of forty-seven (47) PCOS women and forty-seven (47) non-PCOS women with age ranging from 15-50 years was used for the study, to evaluate their knowledge of PCOS and its effect on their reproductive hormones. A structured questionnaire was used to collect data from the respondents. The results showed that PCOS is mainly found among married women within the age of 21 to 30 years. The knowledge of the respondents about PCOS was also found to be very poor with only about 40.43% of them indicating having knowledge about the condition. Finally, PCOS was found to significantly elevate the concentrations of LH and FSH, significantly reduced the concentration of Progesterone, with an insignificant effect on prolactin, with respect to the non-PCOS patients. It was therefore concluded that the women in this study generally have a poor knowledge about the existence of PCOS and that PCOS has the capacity to cause hormonal imbalance.