Polycomb repressive complexes 1 and 2 are each essential for maintenance of X inactivation in extra-embryonic lineages

Masui, Osamu; Corbel, C.; Nagao, Koji; Endo, Takaho A.; Kezuka, Fuyuko; Diabangouaya, Patricia; Nakayama, Manabu; Kumon, Mami; Obuse, Chikashi; Koseki, Haruhiko; Heard, Edith

doi:10.1038/s41556-022-01047-y

Cited by 9 publications

(11 citation statements)

References 51 publications

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“…Deletion of Ring1a/b at E5.5 induced embryonic lethality of both sexes at E8.5. And the female embryos exhibited more severe abnormalities than male embryos, which may be attributable to the impaired XCI in extraembryonic tissues in PRC1-null female embryos ( Masui et al, 2023 ).…”

Section: The Role Of Pcg Complexes In Mammalian Embryonic Developmentmentioning

confidence: 99%

“…A disrupted suppression of Xist -linked genes was observed in extraembryonic lineage at E7.5, which demonstrated that PRC1/2 has a substantial impact on the maintenance of imprinted XCI in extraembryonic lineages. Notably, PRC1 and PRC2 seem to function independently since the loss of PRC1 or PRC2 did not affect the accumulation of H3K27me3 or H2AK119ub in both extraembryonic lineages and embryo at this stage ( Masui et al, 2023 ). While previous study reported that the knockout of Pcgf3/5 gene impaired the deposition of both H2AK119ub1 and H3K27me3 on Xi in mouse embryonic stem cells ( Almeida et al, 2017 ).…”

Section: The Role Of Pcg Complexes In Establishment and Maintenance O...mentioning

confidence: 99%

“…Recently, Masui et al (2023) induced PRCs deletion after the establishment of imprinted XCI ( Ring1a/b deletion at E5.5 or Eed deletion at E3.5), and especially focused on the role of PRC1 and PRC2 in the maintenance of XCI ( Borensztein et al, 2017 ). A disrupted suppression of Xist -linked genes was observed in extraembryonic lineage at E7.5, which demonstrated that PRC1/2 has a substantial impact on the maintenance of imprinted XCI in extraembryonic lineages.…”

Section: The Role Of Pcg Complexes In Establishment and Maintenance O...mentioning

confidence: 99%

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Research advances of polycomb group proteins in regulating mammalian development

Li,

Mo,

Chen

et al. 2024

Front. Cell Dev. Biol.

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Polycomb group (PcG) proteins are a subset of epigenetic factors that are highly conserved throughout evolution. In mammals, PcG proteins can be classified into two muti-proteins complexes: Polycomb repressive complex 1 (PRC1) and PRC2. Increasing evidence has demonstrated that PcG complexes play critical roles in the regulation of gene expression, genomic imprinting, chromosome X-inactivation, and chromatin structure. Accordingly, the dysfunction of PcG proteins is tightly orchestrated with abnormal developmental processes. Here, we summarized and discussed the current knowledge of the biochemical and molecular functions of PcG complexes, especially the PRC1 and PRC2 in mammalian development including embryonic development and tissue development, which will shed further light on the deep understanding of the basic knowledge of PcGs and their functions for reproductive health and developmental disorders.

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Section: The Role Of Pcg Complexes In Mammalian Embryonic Developmentmentioning

confidence: 99%

Section: The Role Of Pcg Complexes In Establishment and Maintenance O...mentioning

confidence: 99%

Section: The Role Of Pcg Complexes In Establishment and Maintenance O...mentioning

confidence: 99%

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Research advances of polycomb group proteins in regulating mammalian development

Li,

Mo,

Chen

et al. 2024

Front. Cell Dev. Biol.

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“…After XCI induction, PRC1 and PRC2 are transported to the Xi chromosome, where Xist accumulates. Mono-ubiquitination of lysine 119 on H2A (H2Aub119) and histone 3 lysine 27 trimethylation (H3K27me3) by PRC1 and PRC2, respectively, directly block transcription ( Aranda et al, 2015 ), induce and maintain heterochromatinization of the Xi chromosome, and suppress gene expression ( Almeida et al, 2017 ; Masui et al, 2023 ). Overall, Xist creates a heritably silent and heterochromatic nuclear territory with a 3D structure distinct from the Xa chromosome.…”

Section: Long Non-coding Rnas (Lncrnas) Are Involved In the Formation...mentioning

confidence: 99%

Potential roles of inter-chromosomal interactions in cell fate determination

Tomikawa

2024

Front. Cell Dev. Biol.

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Mammalian genomic DNA is packed in a small nucleus, and its folding and organization in the nucleus are critical for gene regulation and cell fate determination. In interphase, chromosomes are compartmentalized into certain nuclear spaces and territories that are considered incompatible with each other. The regulation of gene expression is influenced by the epigenetic characteristics of topologically associated domains and A/B compartments within chromosomes (intrachromosomal). Previously, interactions among chromosomes detected via chromosome conformation capture-based methods were considered noise or artificial errors. However, recent studies based on newly developed ligation-independent methods have shown that inter-chromosomal interactions play important roles in gene regulation. This review summarizes the recent understanding of spatial genomic organization in mammalian interphase nuclei and discusses the potential mechanisms that determine cell identity. In addition, this review highlights the potential role of inter-chromosomal interactions in early mouse development.

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“…At the end of the process, about 1000 genes spanning over 167 Mb are silenced [ 102 ]. Noticeably, silenced Xi regions are enriched in H3K27Me3 [ 103 ] and H2AK119ub marks [ 101 ], supporting the crucial role of PcG complexes in this process [ 101 , 104 ]. Epigenomic methods based on ensembles of millions of cells have led to a model in which Xist and its interactors form ribonucleoprotein complexes broadly distributed along Xi [ 103 ].…”

Section: Polycomb Complexes and Xist Regulationmentioning

confidence: 99%

On the Advent of Super-Resolution Microscopy in the Realm of Polycomb Proteins

Nepita

Piazza

Ruglioni

et al. 2023

Biology

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The genomes of metazoans are organized at multiple spatial scales, ranging from the double helix of DNA to whole chromosomes. The intermediate genomic scale of kilobases to megabases, which corresponds to the 50–300 nm spatial scale, is particularly interesting, as the 3D arrangement of chromatin is implicated in multiple regulatory mechanisms. In this context, polycomb group (PcG) proteins stand as major epigenetic modulators of chromatin function, acting prevalently as repressors of gene transcription by combining chemical modifications of target histones with physical crosslinking of distal genomic regions and phase separation. The recent development of super-resolution microscopy (SRM) has strongly contributed to improving our comprehension of several aspects of nano-/mesoscale (10–200 nm) chromatin domains. Here, we review the current state-of-the-art SRM applied to PcG proteins, showing that the application of SRM to PcG activity and organization is still quite limited and mainly focused on the 3D assembly of PcG-controlled genomic loci. In this context, SRM approaches have mostly been applied to multilabel fluorescence in situ hybridization (FISH). However, SRM data have complemented the maps obtained from chromosome capture experiments and have opened a new window to observe how 3D chromatin topology is modulated by PcGs.

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Polycomb repressive complexes 1 and 2 are each essential for maintenance of X inactivation in extra-embryonic lineages

Cited by 9 publications

References 51 publications

Research advances of polycomb group proteins in regulating mammalian development

Research advances of polycomb group proteins in regulating mammalian development

Potential roles of inter-chromosomal interactions in cell fate determination

On the Advent of Super-Resolution Microscopy in the Realm of Polycomb Proteins

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