Polyclonal Antibody Production in Murine Spleen Cells Induced by Staphylococcus

Nakano, Masayasu; Toyoda, Hideko; Tanabe, Masao; Matsumoto, Takao; Masuda, Shōgo

doi:10.1111/j.1348-0421.1980.tb02903.x

Cited by 3 publications

(4 citation statements)

References 32 publications

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“…In the in vivo experiments, the substances from all species of fungi tested showed significant enhancing effect on day 3 (Tables 2-1, 2-2), and this enhancement correlated with their mitogenic activity reported previously (15). The findings that PBA activity was greater on day 3 than on day 5 coincides with other reports (8,9).…”

Section: Suzuki Et Alsupporting

confidence: 80%

“…In addition, it seems that the substances which showed PBA activity (Table l) ought to give rise to nonspecific enhancement of the anti-SRBC PFC response even if their adjuvant effects do not generate it. It is not known what caused the present results, but there are some reports that T cells regulate the generation of polyclonal PFC (4,5,8), and therefore we will examine this question. Furthermore, the chemical properties of these materials will be examined.…”

Section: Suzuki Et Almentioning

confidence: 93%

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Examination of the Polyclonal B Cell Activator and Adjuvant from Fungi

Suzuki

Yonekubo

Yadomae

et al. 1982

Microbiology and Immunology

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Section: Suzuki Et Alsupporting

confidence: 80%

Section: Suzuki Et Almentioning

confidence: 93%

Examination of the Polyclonal B Cell Activator and Adjuvant from Fungi

Suzuki

Yonekubo

Yadomae

et al. 1982

Microbiology and Immunology

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“…This discrepancy is most probably due to the use of serum-free media in their cultures, which resulted in low background levels of PFC of unstimulated cells. The lack of polyclonal activating properties of protein A in mice reported by Nakano et al (34) was most likely due to the use of protein A at concentrations that were too low.…”

Section: Discussionmentioning

confidence: 93%

“…We also evaluated polyclonal activating properties of other staphylococcal cell wall fractions, i.e., teichoic acid (TA), protein A, and purified cell wall (PG-TA complex), since some of these products were shown to act as mitogens or polyclonal activators (or both) of human lymphocytes (10,14,27,42). The reports of their mitogenic or polyclonal activating properties in murine lymphocytes, however, are controversial (10,33,34,46).…”

mentioning

confidence: 99%

Studies on the mechanism of peptidoglycan- and lipopolysaccharide-induced polyclonal activation

Dziarski

1982

Infect Immun

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Peptidoglycan (PG) and lipopolysaccharide (LPS) are T cell-independent B cell mitogens and polyclonal activators in mice. The mechanism of in vitro proliferation and polyclonal activation of mouse splenocytes induced by PG from Staphylococcus aureus and LPS from Escherichia coli was further studied by using [3H]thymidine incorporation and protein A hemolytic plaque assays. Concanavalin A-generated suppressor cells suppressed both polyclonal and proliferative responses induced by PG, LPS, and pokeweed mitogen. The suppression of the proliferative responses was similar for all these mitogens, but was significantly less pronounced than the suppression of the polyclonal antibody response. Polyclonal activation induced by LPS was the most susceptible to suppression by concanavalin A-generated suppressor cells, and the suppression was significantly greater than in the PG-induced polyclonal response. Also, PGinduced polyclonal activation was not susceptible to inhibition by polymyxin B, which is an inhibitor of other B cell mitogens and polyclonal activators. For optimal generation of immunoglobulin-secreting cells, PG or LPS had to be present for at least 48 h after the initiation of the cultures. Removal of the mitogens after 4 or 24 h of incubation resulted in a suboptimal response. For effective induction of the proliferative response, the mitogens had to be present in cultures for over 24 h. Polyclonal-activating properties of staphylococcal cell wall components were also compared. PG was by far the most potent inducer of polyclonal antibodies. Teichoic acid was not active as a polyclonal activator, whereas purified cell wall and protein A were very weak inducers of polyclonal antibodies. These studies demonstrate that PG, in addition to LPS, can be a useful probe for studies on polyclonal activation.

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