Examination of the Polyclonal B Cell Activator and Adjuvant from Fungi

Active site(s) of a B‐cell mitogen, vesiculogen, was investigated by means of chemical and enzymic modifications. Vesiculogen is a non‐dialyzable fraction of a hot water extract from a fungus, Peziza vesiculosa (Yadomae et al, Microbiol. Immunol. 23, 997 (1979)), and was composed of protein (~60%), carbohydrate (~30%), and a small amount of amino sugar, uronic acid, phosphate, and lipid. The mitogenicity was not affected by periodate oxidation, N‐acylation, defatting treatment, destruction of the three dimentional structure, protease digestion, nuclease digestion, N‐bromosuccinimide treatment, and β‐mercaptoethanol treatment. However, the mitogenicity was decreased by a modification of carboxyl groups, such as 1‐ethyl‐3(3‐dimethylamino propyl)‐carbodiimide or dicyclohexylcarbodiimide treatment. Vesiculogen contains a large amount of acidic amino acids. These results suggest that the mitogenicity of vesiculogen is due to the presence of anionic groups, such as aspartic acid and glutamic acid, and that the mitogenic substance is a novel, heat stable polyanionic B‐cell activator obtained from Ascomycotina.

show abstract

Distribution of the mitogenic protein, sclerogen, in extracts from Sclerotinia sclerotiorum IFO 9395 assessed by using immunochemical analysis.

Shinohara¹,

Ohno²,

Yadomae³

1991

Journal of Pharmacobio-Dynamics

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Examination of the Polyclonal B Cell Activator and Adjuvant from Fungi

Cited by 7 publications

References 14 publications

Enhancement of the resistance of tilapia and grass carp to experimental Aeromonas hydrophila and Edwardsiella tarda infections by several polysaccharides

Enhancement of the resistance of tilapia and grass carp to experimental Aeromonas hydrophila and Edwardsiella tarda infections by several polysaccharides

Requirement of Anionic Groups for the Mitogenicity of a Fungal Mitogen, Vesiculogen

Distribution of the mitogenic protein, sclerogen, in extracts from Sclerotinia sclerotiorum IFO 9395 assessed by using immunochemical analysis.

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