Polyclonal antibody assays are better measurements of vitamin d status in asian women than monoclonal

Thomas, S.; Ross, L. J. N.; Ingles, Sue A.; Pandian, Raj; Paulson, Richard J.; Bendikson, Kristin

doi:10.1016/j.fertnstert.2017.02.052

Cited by 2 publications

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“…However, these assays have different selective affinity for VDBP genotypes (which vary by ethnicity), and hence the reported findings may have been skewed. Polyclonal assays are proposed to be superior in measuring VDBP and subsequently calculating free vitamin D, and should therefore be utilised in future studies, particularly in studies of multi-ethnic populations [174][175][176]. Many studies investigating VDBP and pregnancy outcomes have also primarily focused on a direct link between VDBP and pregnancy complications, and most did not consider an alternative indirect link.…”

Section: Critical Appraisal: Limitations and Future Directionsmentioning

confidence: 99%

Vitamin D-Binding Protein in Pregnancy and Reproductive Health

et al. 2020

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Vitamin D-binding protein (VDBP), the main carrier of vitamin D, has recently been implicated in reproductive health and pregnancy outcomes including endometriosis, polycystic ovary syndrome (PCOS), pre-eclampsia, and gestational diabetes mellitus (GDM). Improved methods for measuring VDBP and an increased understanding of its role in biological processes have led to a number of newly published studies exploring VDBP in the context of pregnancy. Here, we synthesize the available evidence regarding the role of VDBP in reproductive health and pregnancy, and we highlight areas requiring further study. Overall, low levels of maternal serum VDBP concentrations have been associated with infertility, endometriosis, PCOS and spontaneous miscarriage, as well as adverse pregnancy outcomes including GDM, pre-eclampsia, preterm birth and fetal growth restriction. However, increased VDBP concentration in cervicovaginal fluid has been linked to unexplained recurrent pregnancy loss and premature rupture of membranes. Some genetic variants of VDBP have also been associated with these adverse outcomes. Further studies using more accurate VDBP assays and accounting for ethnic variation and potential confounders are needed to clarify whether VDBP is associated with reproductive health and pregnancy outcomes, and the mechanisms underlying these relationships.

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Section: Critical Appraisal: Limitations and Future Directionsmentioning

confidence: 99%

Vitamin D-Binding Protein in Pregnancy and Reproductive Health

et al. 2020

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“…First, calculation of free-25(OH)D is largely dependent on the accuracy of total-25(OH)D, albumin, and VDBP measurements. For the latter, genetic variants in VDBP gene mean that measuring VDBP concentrations by a monoclonal antibody ELISA can lead to spuriously low VDBP concentrations in black compared to white subjects [59][60][61], and the same was reported in Asian population [62]. Monoclonal antibody ELISA that recognizes a single epitope near the polymorphic region of VDBP shows preferential binding to the Gc1 s isoform of VDBP that are more commonly associated with European and poorer recognition of the Gc1f phenotype which predominates in individuals of African ancestry [26,29].…”

Section: Discussionmentioning

confidence: 99%

Free 25-hydroxyvitamin-D concentrations are lower in children with renal transplant compared with chronic kidney disease

et al. 2020

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Background Total serum 25-hydroxyvitamin D [25(OH)D] is considered the best marker of vitamin D status and used routinely in clinical practice. However, 25(OH)D is predominantly bound to vitamin D-binding protein (VDBP), and it has been reported that the free-25(OH)D and 25(OH)D loosely bound to albumin fraction correlates better with clinical outcomes. Methods We assessed total-25(OH)D, measured free-25(OH)D, and calculated free-25(OH)D and their relationship with VDBP and biomarkers of mineral metabolism in 61 children (22 CKD 2-3, 18 dialysis, and 21 post-transplant). Results Total-25(OH)D concentrations were comparable across the three groups (p = 0.09), but free-and bioavailable-25(OH)D (free-and albumin-25(OH)D) were significantly lower in the transplant group (both: p = 0.01). Compared to CKD and dialysis patients, the transplant group had significantly higher VDBP concentrations (p = 0.03). In all three groups, total-25(OH)D concentrations were positively associated with measured free-, calculated free-, and bioavailable-25(OH)D. Multivariable regression analysis showed that total-25(OH)D was the only predictor of measured free-25(OH)D concentrations in the dialysis group (β = 0.9; R 2 = 90%). In the transplant group, measured free-25(OH)D concentrations were predicted by both total-25(OH)D and VDBP concentrations (β = 0.6, − 0.6, respectively; R 2 = 80%). Correlations between parathyroid hormone with total-25(OH)D and measured and calculated free-25(OH)D were only observed in the transplant group (all: p < 0.001). Conclusions In transplanted patients, VDBP concentrations were significantly higher compared to CKD and dialysis patients, and consequently, free-25(OH)D concentrations were lower, despite a comparable total-25(OH)D concentration. We suggest that free-25(OH)D measures may be required in children with CKD, dialysis, and transplant, with further research required to understand its association with markers of mineral metabolism.

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Polyclonal antibody assays are better measurements of vitamin d status in asian women than monoclonal

Cited by 2 publications

References 0 publications

Vitamin D-Binding Protein in Pregnancy and Reproductive Health

Vitamin D-Binding Protein in Pregnancy and Reproductive Health

Free 25-hydroxyvitamin-D concentrations are lower in children with renal transplant compared with chronic kidney disease

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