Polybutadiene latex particle size distribution analysis utilizing a disk centrifuge

Verdurmen, Edwin M.; Albers, J.G.; German, Anton L.

doi:10.1007/bf00653310

Cited by 15 publications

(12 citation statements)

References 8 publications

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“…Only the PSD obtained in SB was different with high levels of dimers, trimers, and even tetramers. For the measured multimer pattern, the predicted AHD of the aggregates based on deformable spheres agree well to theoretical values ( D app, i , i = number of particles in the aggregate) monomeric VP ( D VP ) = 81 nm; dimer = 101 nm ( D VP:Dapp,2 = 1:1.25); trimer = 115 nm ( D VP:Dapp,3 = 1:1.42) . The suitability of the prediction based on deformable spheres can be explained by the deformability of influenza A VP shown in previous AUC studies .…”

Section: Resultssupporting

confidence: 81%

Specific ion effects on the particle size distributions of cell culture–derived influenza A virus particles within the Hofmeister series

Heyse

Wolff

Reichl

2016

Engineering in Life Sciences

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o r P e e r R e v i e w 2 PVC, polyvinyl chloride; RPM, revolutions per minute; RT, room temperature; SB, standard buffer; SPA, single particle approximation; VP, Virus particle. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 F o r P e e r R e v i e w 3 Page 2 of 31 Wiley-VCH Engineering in Life Sciences Practical applicationThis work shows the practical application of a differential centrifugal sedimentation (DCS) disc centrifugation method for virus particle size distribution screenings (PSDs). The high resolution and accuracy of the implemented PSD measurement method offered interesting insights on the (aggregation) behavior of influenza A/Puerto Rico/8/34 (H1N1) virus particles in different buffer systems. Therefore, the method's applicability for screening of other biological colloidal particle systems, like vaccine or viral vector formulations, is feasible. Considering the large impact of virus particle size and aggregation behavior on downstream processing yields as well as formulation and blending of vaccines and gene therapy vectors our results address important issues concerning process optimization and product quality. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 Page 3 of 31 Wiley-VCH Engineering in Life Sciences

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Section: Resultssupporting

confidence: 81%

Specific ion effects on the particle size distributions of cell culture–derived influenza A virus particles within the Hofmeister series

Heyse

Wolff

Reichl

2016

Engineering in Life Sciences

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“…For example, DCP is a widely used, high-resolution particle sizing technique that has been used to characterize a wide range of colloidal particles including copolymer latexes, − viruses, − colloidal nanocomposites, ,− protein-coated particles, and various inorganic nanoparticles. ,− DCP is based on the principle of centrifugal sedimentation: particles are radially fractionated within a rotating disk according to their size and relative density; i.e., for particles with uniform density, large particles sediment more quickly than small particles. For calculating accurate particle size distributions using DCP, the effective particle density is an essential input parameter.…”

Section: Resultsmentioning

confidence: 99%

Determining the Effective Density and Stabilizer Layer Thickness of Sterically Stabilized Nanoparticles

et al. 2016

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A series of model sterically stabilized diblock copolymer nanoparticles has been designed to aid the development of analytical protocols in order to determine two key parameters: the effective particle density and the steric stabilizer layer thickness. The former parameter is essential for high resolution particle size analysis based on analytical (ultra)centrifugation techniques (e.g., disk centrifuge photosedimentometry, DCP), whereas the latter parameter is of fundamental importance in determining the effectiveness of steric stabilization as a colloid stability mechanism. The diblock copolymer nanoparticles were prepared via polymerization-induced self-assembly (PISA) using RAFT aqueous emulsion polymerization: this approach affords relatively narrow particle size distributions and enables the mean particle diameter and the stabilizer layer thickness to be adjusted independently via systematic variation of the mean degree of polymerization of the hydrophobic and hydrophilic blocks, respectively. The hydrophobic core-forming block was poly(2,2,2-trifluoroethyl methacrylate) [PTFEMA], which was selected for its relatively high density. The hydrophilic stabilizer block was poly(glycerol monomethacrylate) [PGMA], which is a well-known non-ionic polymer that remains water-soluble over a wide range of temperatures. Four series of PGMAx–PTFEMAy nanoparticles were prepared (x = 28, 43, 63, and 98, y = 100–1400) and characterized via transmission electron microscopy (TEM), dynamic light scattering (DLS), and small-angle X-ray scattering (SAXS). It was found that the degree of polymerization of both the PGMA stabilizer and core-forming PTFEMA had a strong influence on the mean particle diameter, which ranged from 20 to 250 nm. Furthermore, SAXS was used to determine radii of gyration of 1.46 to 2.69 nm for the solvated PGMA stabilizer blocks. Thus, the mean effective density of these sterically stabilized particles was calculated and determined to lie between 1.19 g cm–3 for the smaller particles and 1.41 g cm–3 for the larger particles; these values are significantly lower than the solid-state density of PTFEMA (1.47 g cm–3). Since analytical centrifugation requires the density difference between the particles and the aqueous phase, determining the effective particle density is clearly vital for obtaining reliable particle size distributions. Furthermore, selected DCP data were recalculated by taking into account the inherent density distribution superimposed on the particle size distribution. Consequently, the true particle size distributions were found to be somewhat narrower than those calculated using an erroneous single density value, with smaller particles being particularly sensitive to this artifact.

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“…It reports the weight-average particle diameter, which lies between the number-average and intensity-average diameters obtained from electron microscopy and dynamic light scattering, respectively. DCP has been used to size a wide range of lyophobic colloids, including silica, 1,2 titania, 3,4 barium titanate, 5 carbon nanotubes, 6 pigments, 7 E. coli, 8,9 adenovirus, 10 a range of copolymer latexes, [11][12][13][14][15][16] gold sols 17,18 and various types of nanocomposite particles. [19][20][21][22][23][24][25][26][27][28][29][30][31] In addition, DCP can be used to assess the colloidal stability of aqueous dispersions.…”

Section: Introductionmentioning

confidence: 99%

Correcting for a Density Distribution: Particle Size Analysis of Core–Shell Nanocomposite Particles Using Disk Centrifuge Photosedimentometry

et al. 2012

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Abstract. Many types of colloidal particles possess a core-shell morphology. In this paper we show that, if the core and shell densities differ, this morphology leads to an inherent density distribution for particles of finite polydispersity. If the shell is denser than the core, this density distribution implies an artificial narrowing of the particle size distribution as determined by disk centrifuge photosedimentometry (DCP). In the specific case of polystyrene/silica nanocomposite particles, which consist of a polystyrene core coated with a monolayer shell of silica nanoparticles, we demonstrate that the particle density distribution can be determined by analytical ultracentrifugation and introduce a mathematical method to account for this density distribution by reanalyzing the raw DCP data. Using the mean silica packing density calculated from small-angle xray scattering, the real particle density can be calculated for each data point. The corrected DCP particle size distribution is both broader and more consistent with particle size distributions reported for the same polystyrene/silica nanocomposite sample using other sizing techniques, such as electron microscopy, laser light diffraction and dynamic light scattering. Artifactual narrowing of the size distribution is also likely to occur for many other polymer/inorganic nanocomposite particles comprising a low-density core of variable dimensions coated with a high-density shell of constant thickness, or for core-shell latexes where the shell is continuous rather than particulate in nature.

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Polybutadiene latex particle size distribution analysis utilizing a disk centrifuge

Cited by 15 publications

References 8 publications

Specific ion effects on the particle size distributions of cell culture–derived influenza A virus particles within the Hofmeister series

Specific ion effects on the particle size distributions of cell culture–derived influenza A virus particles within the Hofmeister series

Determining the Effective Density and Stabilizer Layer Thickness of Sterically Stabilized Nanoparticles

Correcting for a Density Distribution: Particle Size Analysis of Core–Shell Nanocomposite Particles Using Disk Centrifuge Photosedimentometry

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