Polyaza functionalized graphene oxide nanomaterial based sensor for Escherichia coli detection in water matrices

Abstract

Water quality is widely discussed owing to its significance in public health due to the inability to access clean water. Waterborne diseases account for the presence of pathogens like Escherichia coli (E. coli) in drinking water in the environmental community. Owing to the rapid increase of such bacterial microorganisms, a cost-effective sensor setup has been developed. Herein, we demonstrate the amine-functionalized graphene oxide (fGO) based 2D nanomaterial used to graft E. coli on its surface. The comparative analysis of the deposition of nanosheets on the glass substrate and PDMS was executed. The impedance variations of GO-based nanosensor at various concentrations of E. coli were performed and their potential difference was recorded. It was observed that the impedance changes inversely with the bacterial concentrations and was fed to the Arduino microcontroller. The experimental setup was standardized for the range of 0.01 Hz to 100 kHz. The obtained analog data was programmed with a microcontroller and the bacterial concentration in colony-forming units was displayed. The real-time analysis showsthe low-level detection of E. coli in aquatic environments. Experiments were conducted using the developed nanosensor to test the efficiency in complex water matrices and whose behavior changes with various physical, chemical, and environmental factors.

Ruthenium Nanoparticle-Immobilized Porous Carbon Nanofibers for Nonenzymatic Dopamine Sensing

“…Carbon nanomaterials are important for chemical and biological sensor applications since their surfaces may be easily modified using a number of covalent or π-stacking methods. − Carbon nanofiber using electrospun polymer nanofibers has been intensively studied thus far as it is a very easy process to produce carbon nanomaterials of diverse forms and configurations. − In recent years, researches have been carried out to change the surface and structural morphology of the carbon nanofibers using various methods such as multilevel nanostructured polymer, chemical vapor deposition (CVD), mixing precursors, chemical treatment, among others. − Porous 1D structure nanomaterials produced by a multilevel nanostructure polymer-based carbonization method have received great attention due to their large specific surface area and high aspect ratio. − For biological and chemical sensor applications, the surface of carbon nanofiber is functionalized by further chemical treatments that employ covalent or π-stacking methods. , …”

Approaches for the Detection of Escherichia coli in Wastewater: A Short Review

“…A basic process centred on absorption techniques was used to explore the detection of E. coli bacteria suspension in Polydimethylsiloxane (PDMS) Glass. [74] The device's microfluid foundations are ideal for miniaturizing measuring tools. The technology, on the other hand, lacks specificity since it can identify other elements as well.…”

“…This location is presently being worked on to minimize its scope. A basic process centred on absorption techniques was used to explore the detection of E. coli bacteria suspension in Polydimethylsiloxane (PDMS) Glass [74] . The device‘s microfluid foundations are ideal for miniaturizing measuring tools.…”