Polyamines, hydrolases (PAP, LAP, SDH, plasmin) TSH, T3, T4 and C-peptide in benign hyperplasia of the prostate

Dunzendorfer, U; Russell, Diane Haddock

doi:10.1007/bf00254408

Cited by 9 publications

(7 citation statements)

References 12 publications

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“…This grdwth plateau was attained in the presence of alterations in the polyamine pathway: The activities of ODC and ARG were both decreased in a dose-dependent fashion in the ventral prostate and coagulating glands, respectively, as was the spermine:putrescine ratio. The relationship between the latter polyamines may be used as an indicator of alterations in glandular growth (Dunzendorfer et al -1981). The decrease in this ratio was primarily due to a relative increase in the tissue levels of putrescine but also due to a decrease in tissue spermine.…”

Section: Discussionmentioning

confidence: 99%

Androgen Interaction with the Polyamine System of the Rat Prostate

et al. 2009

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The ventral and dorsal lobes of the rat prostate contain larger quantities of the aliphatic amines putrescine, spermidine and spennine and higher activities of the enzyme omithine decarboxylase (ODC; EC 4.1.1.17) than other accessory sex glands. In contrast, the coagulating glands and the seminal vesicles contain only small quantities of the amines but the highest activities of the arginase (ARG; EC 3.5.3.1). Lineweaver-Burk plots indicated that the Km-values for ARG in the coagulating gland and ODC in the ventral prostate lobe were 20 mM and 0.2 mM, respectively. Castration decreased ODC and ARG activities to 3 and 50% of control levels, respectively, after 3 days, whilst the Kmvalues were unaffected. Daily administration of 3 mg dihydrotestosterone (DHT) prevented these castrational changes. Oestrogen treatment alone had no effect on the activities of the enzymes, but appeared to exert a synergistic effect with androgen on the ODC. Administration of androgen to intact rats for 7 days caused a dose-related alteration in the ratios of the various amines, particularly the spermine: putrescine ratio. A minor but significant decrease was also recorded in the activity of the ODC, which was mirrored by an increase in the levels of putrescine in the tissue. The data suggest that androgen control of the polyamine pathway is biphasic, first stimulatory and later inhibitory with lesions occurring at the ODC, possibly via short loop feedback of its product putrescine, but also at subsequent enzymic steps in spermidine and spermine biosynthesis. lnteraktion der Androgene mit dem Polyaminstoffwechsel der Rattenprostata Zusammenfassung: Der ventrale und dorsale Lappen der Rattenprostata enthalt grofiere Mengen an aliphatischen Aminen wie Putrescin, Spermidin und Spermin, und auch grofiere Aktivitaten des Enzyms Omithindecarboxylase (ODC; EC 4.1 .I .17) als andere accessorische Geschlechtsdriisen. Im Gegensatz dazu enthalten die Koagulationsdriisen und die Samenblasen nur geringe Mengen an Aminen, aber die hochsten Aktivitaten von Arginase (ARG; EC 3.5.3.1). Berechnungen nach Lineweaver-Burk zeigten, dai3 die Km-Werte fur ARG in der Koagulationsdriise und ODC im ventralen Prostatalappen 20 mM beziehungsweise 50% der Kontrollgruppenwerte nach 3 Tagen, wahrend die Km-Werte nicht beeinflufit waren. Die tagliche Gabe von 3 mg Dihydrotestosteron verhinderte die Veranderungen unter Kastration. Alleinige ostrogenbehandlung hatte keinen Effekt auf die Enzymaktivitaten, abei-es zeigte sich ein synergistischer Effekt zusammen mit Androgen auf die ODC. Wurden gesunde Ratten iiber 7 Tage mit Androgenen behandelt, bewirkte das eine dosisabhangige Veranderung der Aminkonzentrationen, im besonderen das Verhaltnis Sper-Androgen Interaction 135 min zu Putrescin. Ein geringer jedoch signifikanter Abfall wurde auch fur die ODC-Aktivitat registriert, welches sich in einem Anstieg der Putrescin-Werte im Gewebe widerspiegelte. Diese Daten lassen vermuten, d& die durch Androgene bewirkte Kontrolle auf den Polyaminstoffwechsel biphasisch ist, zu Begi...

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Section: Discussionmentioning

confidence: 99%

Androgen Interaction with the Polyamine System of the Rat Prostate

et al. 2009

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“…In animal models, the levels of thyroid hormone determine the size of the prostate (Howland and Ibrahim, 1974). In fact, thyroid hormone has been regarded as a factor in the regulation of the benign prostatic hyperplasia and prostate cancer (Dunzendorfer et al, 1981; Lehrer et al, 2002). Recently, studies have shown that T3 can modulate cell growth and potentiates the androgen‐mediated transcription of PSA gene in LNCaP cells (Esquenet et al, 1995; Zhang et al, 1999; Zhu and Zhang, 2001).…”

Section: Discussionmentioning

confidence: 99%

Cell Growth Effects of Triiodothyronine and Expression of Thyroid Hormone Receptor in Prostate Carcinoma Cells

Hsieh

Juang

2005

Journal of Andrology

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Thiiodothyronine (T3) plays an important role in the regulation of cell growth and differentiation. In this study, we show the different effects of T3 on cell growth response and expression of the thyroid hormone receptor in human prostate cell lines from normal to hormonal refractory metastatic cancer cells. Although the thyroid hormone receptor (TR␤1) ubiquitously express in human prostatic epithelium cell lines (PZ-HPV-7, CA-HPV-10, LNCaP, DU145, PC-3), T3 did not show any effect on the cell proliferation of prostatic cell lines except LNCaP cells in vitro. Immunoblot assay revealed that PZ-HPV-7 and CA-HPV-10 cells express 5-10-fold of TR␤1 more than LNCaP cells; however, the immunocytochemical staining and immunoblot assay of cellular fractions suggested the TR␤1 is located on the cell nuclear membrane of PZ-HPV-7 and CA-HPV-10 cells. Our results suggested that T3 upregulates cellular proliferation on LNCaP cells but not other prostatic carcinoma cells and PZ-HPV-7 and CA-HPV-10 cells express the novel TR␤1, which locates at cell nuclear membrane.

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“…Although thyroid hormone is an important regulator of growth and differentiation in many cell types, the critical influence of thyroid hormone on the growth and development of the reproductive system are, as yet, not well understood [1]. An early study suggested that thyroid hormone contributes to the regulation of benign prostatic hyperplasia (BPH) and prostate cancer [2]. A recent study indicates that men with BPH or prostate cancer have significantly elevated serum triiodothyronine (T3) levels [3,4].…”

Section: Introductionmentioning

confidence: 99%