Androgen Interaction with the Polyamine System of the Rat Prostate

Rui, Hallgeir; Brekke, I.; Mørkås, L.; Purvis, K.

doi:10.1111/j.1439-0272.1987.tb01872.x

Cited by 8 publications

(3 citation statements)

References 23 publications

(20 reference statements)

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“…Polyamines and transglutaminase in rat prostate tissues. This study confirmed the results from previous reports Mann & Lutwak-Mann;1981;Rui et al, 1987) in which it was shown that polyamines are (Rui et al, 1987).…”

Section: Discussionsupporting

confidence: 93%

Polyamines and transglutaminase actions: Polyamine und Transglutaminase-Wirkungen

Romijn

2009

Andrologia

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Summary Polyamines (putrescine, spermidine and spermine), secreted by the prostate gland, occur in high concentrations in the seminal fluid of many species, including man and rat. The physiological significance of seminal polyamines is still obscure, however. It has been postulated that polyamines may serve as amine donor substrates for transglutaminases (TGases), enzymes catalyzing protein crosslinking by the formation of γ‐glutamyllysine or bis(γ‐glutamyl)polyamine cross‐bridges. We have analyzed TGase‐activities and polyamine content of the various rat prostate lobes and rat seminal vesicles. Highest TGase activities were observed in the coagulating gland (anterior prostate) and the dorsolateral prostate, whereas very little TGase activity was present in the ventral prostate gland. In contrast, polyamine concentrations were highest in the ventral prostate but low in coagulating glands. Seminal vesicles, and in particular seminal vesicle secretions, contained low polyamine levels and intermediate TGase activity. Levels of protein‐bound polyamines, not extractable by perchlorid acid, did not correlate with TGase‐activities in ventral prostate and coagulating glands, suggesting an extracellular rather than intracellular function of prostatic TGase and polyamines. The observation that Km‐values of rat prostate TGase for all three polyamines (57–120 μM, using N,N‐dimethylated casein as protein substrate) were well below seminal polyamine concentrations is compatible with a regulatory role of polyamines in the process of seminal clot formation. Zusammenfassung Polyamine (Putrescin, Spermidin und Spermin), von der Prostata sezerniert, werden in hohen Konzentrationen in der Samenflüssigkeit vieler Spezies, Mensch und Ratte eingeschlossen, angetroffen. Die physiologische Bedeutung ihrer Anwesenheit im Samen ist aber unklar. Man hat postuliert, daß Polyamine als Amingruppen‐Donoren für das Enzym Transglutaminase (TGase) auftreten können, welches seinerseits cross‐linking von Proteinen mit Hilfe von γ‐Glutamyl‐Lysin oder bis(γ‐Glutamyl)‐Polyamin‐Brücken katalysiert. Wir haben die Aktivitäten von TGase sowie die Polyamin‐Gehalte in den verschiedenen Lappen der Rattenprostata und in den Samenblasen bestimmt. Die höchsten TGase‐Aktivitäten wurden in der Koagulationsdrüse und im dorsolateralen Prostatalappen aufgefunden, während die ventralen Prostatalappen nur wenig Aktivität aufwiesen. Im Gegensatz dazu wurden die höchsten Polyamin‐Spiegel in der ventralen Prostata und die niedrigsten in der Koagulationsdrüse gefunden. Die Bläschendrüsen, und insbesondere ihre Sekrete, enthielten niedrige Polyamin‐Konzentrationen und eine mäßige TGase‐Aktivität. Der Gehalt an proteingebundenem Polyamin (nicht extrahierbar mit Perchlorsäure) korrelierte nicht mit den TGase‐Aktivitäten der ventralen Prostatalappen und Koagulationsdrüsen. Dieser Befund läßt vermuten, daß Prostata‐TGase und ‐Polyamine ihre Funktion überwiegend extrazellulär ausüben. Die Beobachtung, daß die Km‐Werte von Prostata‐TGase für die drei Polyamine (57–120 μM mit N,N‐...

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Section: Discussionsupporting

confidence: 93%

Polyamines and transglutaminase actions: Polyamine und Transglutaminase-Wirkungen

Romijn

2009

Andrologia

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“…Furthermore, in kidneys of female mice, the addition of testosterone has led to induction of ODC and AII and an associated reduction in OAT levels (35,36). As expected, castration of male mice has shown the opposite effect with a decline in levels of ODC and AII and a simultaneous increase in OAT (36,37). Consistent with these studies, we found ODC and even more dramatically AII expression to be significantly higher in the androgen-dependent prostate cancer cell lines compared to the androgen-independent lines.…”

Section: Regulation Of Arginase By Androgensmentioning

confidence: 59%

Expression of arginase II in prostate cancer

Mumenthaler

Tze

et al. 2008

Int J Oncol

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Previous reports have shown elevated arginase activity in prostate cancer patients. This study was designed to compare expression levels of arginase II (AII) in various human prostate cancer cell lines and tissues. Expression levels of AII and other enzymes involved in arginine metabolism were examined in androgen-dependent (LNCaP, LAPC-4) and androgen-independent (PC3, DU145, CL-1, CL-2) prostate cancer cell lines by real-time RT-PCR and Western blot analysis. Further expression analysis of AII was accomplished by immunohistochemical staining of a tissue microarray comprised of 246 primary prostatectomy specimens. In addition, polyamine levels were measured within the prostate cancer cell lines by HPLC. Total polyamines were significantly lower in the androgen-dependent cell lines compared to the androgen-independent cell lines. AII expression was found to be most prominent in the androgen-dependent cell lines and least prominent in the androgen-independent cell lines. Additionally, we found expression of ornithine aminotransferase (OAT), an enzyme also responsible for ornithine production, to be inversely correlated with AII expression. The tissue microarray data revealed that the highest AII expression was seen in BPH, followed by PIN and normal samples, with the lowest expression levels observed in prostate cancer tissues. Moreover, we observed an expression gradient across Gleason grades revealing stronger AII expression in low-grade tumors. The polyamine data, combined with the expression analysis studies, support a possible correlation between AII, OAT, and polyamine synthesis. Based on these results, arginase II expression may play a role in prostate cancer progression. More specifically, the elevated AII expression seen in androgen-dependent and in more differentiated prostate cancers suggests that AII could be a potentially useful marker of early stage prostate adenocarcinoma.

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“…Extrahepatic arginase may play a role in reactions other than the urea cycle. In mammals, it is found in prostate (Gotoh, Araki, & Mori, ; Vockley et al, ), testis (Nadolska‐Lutyk, Graboń, & Porembska, ), epididymis (Reddi, Knox, & Herzfeld, ), seminal plasma (Rui, Brekke, Mørkås, & Purvis, ) and human sperm cells (Papp, Grof, Molnar, & Jambor, ). Moreover, ornithine synthesised from L‐arginine is a precursor for polyamine synthesis.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the role of L‐arginine on spermatological parameters, seminal plasma nitric oxide levels and arginase enzyme activities in rams

et al. 2019

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The purpose of this study is to investigate the effects of L‐arginine on spermatological parameters, seminal plasma nitric oxide levels and arginase enzyme activities. Fertile rams that are 2–3 years old and weighing 50–60 kg were used as material. The semen was collected by artificial vagina at 1st, 4th, 24th, 48th, 72nd, 96th and 120th hours for the control group before L‐arginine administration. For treatment groups, L‐arginine was administered intraperitoneally at a dose of 5 mg kg−1 bw−1 and semen was collected at the time point described for the control group. Spermatological characteristics of semen samples (semen volume, pH, sperm motility, concentration and abnormal sperm rate), seminal plasma nitric oxide levels and arginase enzyme activities were determined. Increased seminal plasma nitric oxide level (p < .01), seminal plasma arginase activity (p < .01), semen volume (p < .05), semen mass activity (p < .05), sperm motility (p < .05) and concentration (p < .01) and decreased abnormal sperm rate (p < .05 and p < .01) were observed by L‐arginine administration. In conclusion, it may be concluded that L‐arginine application in rams during the breeding season may have positive effects on rams' reproductive performance.

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Androgen Interaction with the Polyamine System of the Rat Prostate

Cited by 8 publications

References 23 publications

Polyamines and transglutaminase actions: Polyamine und Transglutaminase-Wirkungen

Polyamines and transglutaminase actions: Polyamine und Transglutaminase-Wirkungen

Expression of arginase II in prostate cancer

Evaluation of the role of L‐arginine on spermatological parameters, seminal plasma nitric oxide levels and arginase enzyme activities in rams

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