Polyamines and Morphogenesis in Monocots: Experimental Systems and Mechanisms of Action

Tiburcio, Antonio F.; Campos, José Luis; Figueras, X.; Marcé, M.; Capell, Teresa; Riera, Rojas; Besford, R. T.

doi:10.1007/978-1-4899-1265-7_6

Cited by 3 publications

(1 citation statement)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The biphasic nature of the concentrations of these metabolites may, in part, be interpreted in context with the requirements of polyamines during embryo development, Polyamines and their respective biosynthetic enzymes increased dtiring embryo development (Montague et al, 1978, Feinberg et al, 1984, Feirer et al, 1984, Mengoli et al, 1989, Robie and Minocha 1989 and are required for continued development (Feirer et al, 1984, Robie andMinocha 1989), Also the itihibition of polyatnine synthetic pathways can lead to changes in embryo development (Robie and Minocha 1989), GABA is known to be a catabolite of polyamines of which either Om or Arg can be precursors (Bagni et al, 1993, Tiburcio et al, 1993, However, our present data do not allow us to conclude whether this route contributed to GABA formation in these experiments. Therefore, it is tempting to speculate, based on this and previous work, that the increase in omithine cycle metabolites may be related to increases in polyamines.…”

Section: Gaba Alamentioning

confidence: 99%

Stage‐specific nitrogen metabolism in developing carrot somatic embryos

Joy

McLntyre

Vogel

et al. 1996

Physiologia Plantarum

View full text Add to dashboard Cite

The physiology of individual somatic embryo developmental stages otDaucus carota L. was examined by in vivo nuclear magnetic resonance (NMR) spectroscopy, amino acid analysis and 14C‐labeling. 15N NMR spectroscopy was used to examine the uptake and incorporation of 15N isotopically labeled inorganic nitrogen sources. NMR spectra of proembryogenic masses (PEMs) contained resonances for histidine, amino sugars, glutamine, arginine, urea, alanine. α‐amino nitrogen, serine, aliphatic amines and several unknowns. Similar resonances were found in various embryo developmental stages. However, resonances for arginine and aliphatic amines peaked during globular and torpedo stages and substantially decreased in germinating stage embryos. The dominant resonances observed in non‐embryogenic cells and germinating embryos were glutamine and α‐amino nitrogen. Amino acid analysis of the various embryo stages showed that glutamate, glutamine and arginine were the major contributors to the soluble amino acid profiles. During development, glutamate and glutamine continued to increase in concentration whereas arginine and its related metabolites (i.e. ornithine and y‐aminobutyric acid [GABA]) were biphasic; increasing in globular and torpedo stage embryos and decreasing in germinating embryos. Carbon‐14 labeling indicated that labeled glutamine pools in non‐embryogenic and germinating embryos were greatest compared to other embryo stages, whereas labeled GABA pools were greatest in globular and torpedo stage embryos. Taken together, these data indicate that the physiology of each embryo developmental stage is distinct. They also suggest that during somatic embryo development, a switch takes place in metabolism whereby the glutamine synthetase/glutamate synthase (GS/GOGAT) pathway is predominant in non‐embryogenic cells and germinating stage embryos. Furthermore, during early to mid‐embryo development (PEMs, globular and torpedo stage embryos), metabolism utilizing the omithine cycle is enhanced and predominant.

show abstract

Section: Gaba Alamentioning

confidence: 99%

Stage‐specific nitrogen metabolism in developing carrot somatic embryos

Joy

McLntyre

Vogel

et al. 1996

Physiologia Plantarum

View full text Add to dashboard Cite

show abstract

An Assessment of Possible Factors Contributing to Recalcitrance of Plant Protoplasts

Roubelakis‐Angelakis

1993

Morphogenesis in Plants

View full text Add to dashboard Cite

Potential Applications of Polyamines in Agriculture and Plant Biotechnology

Tiburcio

Alcázar

2017

Methods in Molecular Biology

View full text Add to dashboard Cite

The polyamines putrescine, spermidine and spermine have been implicated in a myriad of biological functions in many organisms. Research done during the last decades has accumulated a large body of evidence demonstrating that polyamines are key modulators of plant growth and development. Different experimental approaches have been employed including the measurement of endogenous polyamine levels and the activities of polyamine metabolic enzymes, the study of the effects resulting from exogenous polyamine applications and chemical or genetic manipulation of endogenous polyamine titers. This chapter reviews the role of PAs in seed germination, root development, plant architecture, in vitro plant regeneration, flowering and plant senescence. Evidence presented here indicates that polyamines should be regarded as plant growth regulators with potential applications in agriculture and plant biotechnology.

show abstract

Polyamines and Morphogenesis in Monocots: Experimental Systems and Mechanisms of Action

Cited by 3 publications

References 69 publications

Stage‐specific nitrogen metabolism in developing carrot somatic embryos

Stage‐specific nitrogen metabolism in developing carrot somatic embryos

An Assessment of Possible Factors Contributing to Recalcitrance of Plant Protoplasts

Potential Applications of Polyamines in Agriculture and Plant Biotechnology

Contact Info

Product

Resources

About