Nuclear Magnetic Resonance (NMR) spectroscopy was used to probe the primary structure of the glucan pullulan. Carbon-13, proton and phospliorus-31 NMR spectra in aqueous solution of the intact polysaccharide obtained from three different sources indicate that significant suuctural differences can occur among these samples. In particular, two samples consist solely of a(1+6) linked maltotriose units, in accord with the generally accepted structure. However a third sample has fewer a(l-+6) linkages and appears to consist of higher a( 1 + 6) linked maltooligosaccharides in addition to maltotriose. Unlike the other samples. this sample was also phosphorylated. Enzymatic digzstion of pullulan followed by NMR spectroscopy using pullulanase confirmed these results, as did HPLC analysis of the resulting digest, which showed the presence of maltooligosaccharides consisting of ten and more glucose units. However, HPLC analysis of acid hydro1 ysed pullulan indicated that no sugars other than glucose were present in any of the samples examined.
The physiology of individual somatic embryo developmental stages otDaucus carota L. was examined by in vivo nuclear magnetic resonance (NMR) spectroscopy, amino acid analysis and 14C‐labeling. 15N NMR spectroscopy was used to examine the uptake and incorporation of 15N isotopically labeled inorganic nitrogen sources. NMR spectra of proembryogenic masses (PEMs) contained resonances for histidine, amino sugars, glutamine, arginine, urea, alanine. α‐amino nitrogen, serine, aliphatic amines and several unknowns. Similar resonances were found in various embryo developmental stages. However, resonances for arginine and aliphatic amines peaked during globular and torpedo stages and substantially decreased in germinating stage embryos. The dominant resonances observed in non‐embryogenic cells and germinating embryos were glutamine and α‐amino nitrogen. Amino acid analysis of the various embryo stages showed that glutamate, glutamine and arginine were the major contributors to the soluble amino acid profiles. During development, glutamate and glutamine continued to increase in concentration whereas arginine and its related metabolites (i.e. ornithine and y‐aminobutyric acid [GABA]) were biphasic; increasing in globular and torpedo stage embryos and decreasing in germinating embryos. Carbon‐14 labeling indicated that labeled glutamine pools in non‐embryogenic and germinating embryos were greatest compared to other embryo stages, whereas labeled GABA pools were greatest in globular and torpedo stage embryos. Taken together, these data indicate that the physiology of each embryo developmental stage is distinct. They also suggest that during somatic embryo development, a switch takes place in metabolism whereby the glutamine synthetase/glutamate synthase (GS/GOGAT) pathway is predominant in non‐embryogenic cells and germinating stage embryos. Furthermore, during early to mid‐embryo development (PEMs, globular and torpedo stage embryos), metabolism utilizing the omithine cycle is enhanced and predominant.
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