Polyamine levels in the seagrass Cymodocea nodosa

Marian, Fernando D.; García‐Jiménez, Pilar; Robaina, Rafael R.

doi:10.1016/s0304-3770(00)00111-x

Cited by 17 publications

(11 citation statements)

References 22 publications

(23 reference statements)

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“…The present results (Table 1) show the presence of Put, Spd and Spm in the marine algae studied. These results add to previous reports on the presence of PAs in other macroalgal species (Badini et al 1994, Lee 1998) and our recent results for the marine seagrass Cymodocea nodosa (Marián et al 2000). In all cases, the concentrations were within the range commonly found for PAs in higher plants (Harkess et al 1992, Biondi et al 1993.…”

Section: Discussionsupporting

confidence: 89%

Polyamines in marine macroalgae: Levels of putrescine, spermidine and spermine in the thalli and changes in their concentration during glycerol-induced cell growth in vitro

Marian¹,

García‐Jiménez²,

Robaina³

2000

Physiol Plant

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growth and morphogenesis and also increased the free and The levels of putrescine (Put), spermidine (Spd) and spermine bound PA levels in the sporelings. Tracer experiments using 70 (Spm) were analyzed in naturally collected samples of the marine macroalgae Dyctiota dichotoma, Gelidium canariensis kBq [U-14 C]-glycerol showed significant quantities of radioactivity in Put, Spd and Spm after 20 h of incubation. The effects and Grateloupia doryphora. Polyamines (PAs) appeared in free (35-134 mg g − 1 fresh weight) and bound TCA-insoluble of glycerol on growth were inhibited by the ornithine decarboxylase (EC 4.1.

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Section: Discussionsupporting

confidence: 89%

Polyamines in marine macroalgae: Levels of putrescine, spermidine and spermine in the thalli and changes in their concentration during glycerol-induced cell growth in vitro

Marian¹,

García‐Jiménez²,

Robaina³

2000

Physiol Plant

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“…As in higher plants PAs have been shown to be involved in algal cell growth, cell division and the maturation of reproductive structures (Marian et al 2000;Theiss et al 2002;Guzman-Uriostegui et al 2002;Sacramento et al 2004). The importance of PAs in the membranes of photosynthetic complexes has also been demonstrated in unicellular alga (Beigbeder et al 1995;Doernemann et al 1996;Sfichi et al 2004;Sfichi-Duke et al 2008).…”

Section: Introductionmentioning

confidence: 98%

UV-B radiation induces changes in polyamine metabolism in the red seaweed Porphyra cinnamomea

et al. 2011

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Early investigations on the productivity of intertidal seaweeds found that, unlike some seaweeds, members of the genus Porphyra, a Rhodophyte, could tolerate physical stressors such as ultraviolet-B radiation (UV-B) both during immersion and when exposed to air. Increased stress tolerance was thought to be due to an unknown mechanism that operated at the thylakoid level. As recent research has shown that polyamines (PAs), bound to the thylakoid membranes of chloroplasts, play a critical role in protecting the photosynthetic apparatus from high-light and UV damage in both higher plants and in unicellular algae, we investigated PA metabolism in Porphyra cinnamomea exposed to UV-B. Our results show that PA biosynthesis was significantly upregulated in P. cinnamomea in response to UV-B, with the greatest proportional increases being in bound soluble putrescine (PUT), which increased by over 200%, in bound soluble spermidine (SPD) and spermine (SPM) which both increased by more than 150% and in bound insoluble SPM which increased by more than 120%. As PAs can be synthesised from ornithine via ornithine decarboxylase (ODC) or from arginine via arginine decarboxylase (ADC) we investigated the pathway via which polyamines were synthesised in P. cinnamomea. While exposure to UV-B caused increases in the activities of both ADC and ODC, the increase in ADC activity was 10 fold greater than that of ODC, suggesting that the ADC pathway was the principle route by which PA levels increased in response to UV-B. Mechanisms of PA mediated UV-B protection are discussed.

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“…In algae, there are data on the endogenous levels of polyamines in several green, red and brown algae (Badini et al 1994;Marián et al 2000a). These polyamines are involved in cell division (Cohen et al 1984;García-Jiménez et al 1998;Marián et al 2000b;Voigt et al 2000;Theiss et al 2002), and in response to hyposaline stress (Lee 1998;García-Jiménez et al 2007).…”

Section: Introductionmentioning

confidence: 99%

The polyamine spermine induces cystocarp development in the seaweed Grateloupia (Rhodophyta)

2007

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Polyamines such as putrescine, spermidine and spermine are ubiquitous aliphatic amines involved in reproductive events in plants and algae, and first become evident through changes in endogenous levels during reproductive development. To examine whether the differences observed in polyamines, during carposporogenesis, in the red alga Grateloupia, followed a specific pattern as is seen in other organisms, infertile axes (i.e. not showing cystocarps) were excised from the same holdfast of female fertilized individuals (i.e. showing cystocarps in other axes), and cultivated until the cystocarps became visible. Changes in the endogenous levels of free putrescine, spermidine and spermine were monitored over the 8 days of culture. The activity of enzymes related to polyamine metabolism, such as L-ornithine decarboxylase (ODC), diamine oxidase and polyamine oxidase, was measured at the beginning and end of the experimental period. Up to 50% of the infertile axes became fertile and produced cystocarps at a density of 1.91 ± 0.1 cystocarps mm À2 after 8 days. The endogenous content of spermine increased markedly over the first 5 days of culture, then decreased to the initial level by day 8.Spermidine followed a similar pattern to spermine, whereas putrescine remained at high levels, until day 5 when it decreased abruptly. The activity of ODC was less on day 8 than on day 0, whereas the activities of diamine oxidase and polyamine oxidase increased. In parallel experiments with explants from infertile axes, exogenously added spermine (10 À6 M) increased the number of cystocarps, and reversed the effect of cyclohexylamine (CHA), which is known to inhibit polyamine synthesis in Grateloupia. Serial sectioning and microscopic observation of specimens from explants cultivated in 10 À6 M spermine indicated that cystocarp development was induced. The results suggest that, during transition from infertile to fertile spermine is accumulated, thus favouring the development of cystocarps, given the presumed role of spermine as an inducing agent.

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Polyamine levels in the seagrass Cymodocea nodosa

Cited by 17 publications

References 22 publications

Polyamines in marine macroalgae: Levels of putrescine, spermidine and spermine in the thalli and changes in their concentration during glycerol-induced cell growth in vitro

Polyamines in marine macroalgae: Levels of putrescine, spermidine and spermine in the thalli and changes in their concentration during glycerol-induced cell growth in vitro

UV-B radiation induces changes in polyamine metabolism in the red seaweed Porphyra cinnamomea

The polyamine spermine induces cystocarp development in the seaweed Grateloupia (Rhodophyta)

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