Polyamide 6/chitosan nanofibers as support for the immobilization of Trametes versicolor laccase for the elimination of endocrine disrupting chemicals

Maryšková, Milena; Ardao, Inés; García‐González, Carlos A.; Martinová, Lenka; Rotkova, Jana; Ševců, Alena

doi:10.1016/j.enzmictec.2016.03.001

Cited by 79 publications

(38 citation statements)

References 38 publications

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“…In addition, electrospun nanofibers have the potential to be applied as good sorbents for the extraction, removal, and filtration of heavy‐metal ions, dyes, pesticides, and phenolic endocrine disruptor pollutants from real samples . Furthermore, many functional additives, including dyes, biomolecules, and nanoparticles (NPs), have been incorporated into electrospun nanofibers to make them an excellent adsorption medium for the removal of contaminants …”

Section: Introductionmentioning

confidence: 99%

Simple preparation and characterization of molecularly imprinted nylon 6 nanofibers for the extraction of bisphenol A from wastewater

Ardekani

Borhani

Rezaei

2018

J of Applied Polymer Sci

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Nylon 6 nanofibers incorporated with molecularly imprinted polymers (MIPs) were successfully fabricated by electrospinning with fiber diameters in the range 80-145 nm. Then, they were used as a new material for the extraction of selected bisphenol A (BPA) in water samples. Field emission scanning electron microscopy images revealed that the nanofibers had a smooth morphology with a good incorporation of MIPs. The Fourier transform infrared and energy-dispersive X-ray spectroscopy results also confirmed the formation of the MIPs in the nanofibers. Furthermore, Raman spectroscopy showed that the crystalline structure of the pristine nylon 6 nanofiber was a kind of α form, and the incorporation of MIPs led to a γ-form structure in the nanofibers; this proved the interactions between nylon 6 and the MIPs. Adsorption studies also confirmed that the adsorption efficiency of BPA onto the molecularly imprinted polymer nanofibers (MIP-NFs; 83.5%) was much greater than that onto nonimprinted polymer nanofibers (NIP-NFs; 36.8%). Also, the imprinting factor was 3.4; this strongly implied the successful formation of molecularly imprinted cavities on the MIP-NFs with a strong affinity to BPA. The maximum adsorption capacity of the MIP-NFs was 103.8 mg/g.

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Section: Introductionmentioning

confidence: 99%

Simple preparation and characterization of molecularly imprinted nylon 6 nanofibers for the extraction of bisphenol A from wastewater

Ardekani

Borhani

Rezaei

2018

J of Applied Polymer Sci

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“…However, in physical adsorption approaches, BSA can desorb enzymes because of their relatively low affinity for cellulose . BSA was also used to passivate chitosan nanofibers before covalent attachment of laccase, but retained only about 7 % of the original laccase activity . Our two‐step one‐pot approach for enzyme stabilization has not been reported, and appears essential for the development of stable and functional enzyme‐paper biocatalysts, biosensors or biomaterials.…”

Section: Methodsmentioning

confidence: 99%

“…Although immobilized laccase stability at 4 °C is reported, 25 °C stability is not well known (Figure C). Additionally, reports are sometimes unclear about how much activity is retained relative to the unmodified enzyme . Thus, activity retention at 25 and 4 °C was measured as a function of storage time, and was compared both to unmodified enzyme (Figure ) and to each respective sample, at day 0 (Supporting Information, Figure S7).…”

Section: Methodsmentioning

confidence: 99%

A Modular Approach for Interlocking Enzymes in Whatman Paper

et al. 2018

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A potentially universal approach is presented for enzyme attachment to cellulose that significantly enhances enzyme stability while retaining high activity, and involves no chemical functionalization of cellulose. Bovine serum albumin (BSA) was interlocked in cellulose to form a protein‐friendly surface (named BSA‐Paper), while also providing COOH and NH2 groups for subsequent attachment of enzymes. The desired enzyme is then mixed with additional BSA and interlocked on BSA‐Paper. The second BSA layer dilutes and crosslinks the enzyme for improved stability. Laccase was tested as a model enzyme for interlocking on BSA‐Paper, and was found to retain over 100 % activity and was 240 times more stable at 25 °C (half life=180 d) than laccase. This new approach was also tested with a few other enzymes with encouraging results, thus providing a potentially universal method for stabilization of enzymes on cellulose with retention of high activities.

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“…PA6 is a semicrystalline polymer with good tensile properties, and it has been used for textiles, adhesives, and biomedical purposes . PA6/chitosan nanofibers were used as support to immobilize Trametes versicolor laccase for the elimination of endocrine‐disrupting chemicals . Chymotyrpsin was immobilized on hierarchical nylon 6,6 electrospun nanofibers, and an enhanced enzyme performance was reported .…”

Section: Introductionmentioning

confidence: 99%

Cellulose monoacetate/polycaprolactone and cellulose monoacetate/polycaprolactam blended nanofibers for protease immobilization

Aykut

Sevgi

Demirkan

2017

J of Applied Polymer Sci

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Enzymes can be used multiple times when they are immobilized on a support. More enzymes can be immobilized on a surface when nanofibers are used as a supporting surface because the specific surface area increases tremendously. In this regard, polycaprolactam/cellulose monoacetate (PA6/CMA) and polycaprolactone/cellulose monoacetate (PCL/CMA) blended nanofibers (NFs) were prepared via an electrospinning process. Protease enzymes were immobilized on neat PA6, PCL, PA6/CMA, and PCL/CMA nanofibers and glutaraldehyde (GA) activated analogs through the physical adsorption method. The immobilized enzyme activity was measured by using a casein substrate, and the results were compared with free enzyme activity. Among all of the samples, the highest immobilization yield of about 82% was obtained with GA‐activated neat PCL NF samples. The best remaining activity of the immobilized enzymes on pure CMA NFs was found to be 59% after seven reuses. Even after nine reuses, enzyme activities are still observed on the CMA NF samples. It was expected that the addition of CMA in PCL and PA6 NFs would increase the reusability number to reach the reusability of CMA NFs, but it was not significantly enhanced. If CMA chains could be mostly collected on the sheath or close to the sheath of the NFs during the electrospinnig process, this target could be achieved. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 45479.

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Polyamide 6/chitosan nanofibers as support for the immobilization of Trametes versicolor laccase for the elimination of endocrine disrupting chemicals

Cited by 79 publications

References 38 publications

Simple preparation and characterization of molecularly imprinted nylon 6 nanofibers for the extraction of bisphenol A from wastewater

Simple preparation and characterization of molecularly imprinted nylon 6 nanofibers for the extraction of bisphenol A from wastewater

A Modular Approach for Interlocking Enzymes in Whatman Paper

Cellulose monoacetate/polycaprolactone and cellulose monoacetate/polycaprolactam blended nanofibers for protease immobilization

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