Polyadenylic Acid in Visna Virus RNA

Gillespie, David; Takemoto, Kenneth K.; Robert, Marjorie; Gallo, Robert C.

doi:10.1126/science.179.4080.1328

Cited by 37 publications

(8 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cytolytic changes, including the development of large multinueleated giant cells, accompany virus multiplication. Visna virus resembles the tumorigenic RNA viruses in size, morphogenesis (6,45), sensitivities to chemical and physical agents (46), the presence of an l~NA-dependent I)NA polymerase (21,37,42), and rapidly sedimenting 60--70S single-stranded I~NA with long polyadenylate stretches (2,9,14,22). Preliminary analysis of visna virus proteins indicates a similarity in the number and gel mobility patterns to the proteins of the oncogenic RNA viruses (28).…”

Section: Introductionmentioning

confidence: 99%

Visna virus-induced fusion of continuous simian kidney cells

August

Harter

1974

Archiv f Virusforschung

View full text Add to dashboard Cite

SummarySyncytium formation was observed in four continuous monkey kidney cell lines (BS-C-1, VEI~O, LLC, MK2 and CV-1) after inoculation with visna virus a~ high multiplicity. Visna virus-induced cell fusion occurred most rapidly and extensively in BS-C-1 and VEI~O cells; it was least prominent in CV-1 cells. No evidence of significant visna virus multiplication was detected in any of the four monkey kidney cell lines. BS-C-1 cells were successfully used as an indicator cell line in a visna virus plaque assay.

show abstract

Section: Introductionmentioning

confidence: 99%

Visna virus-induced fusion of continuous simian kidney cells

August

Harter

1974

Archiv f Virusforschung

View full text Add to dashboard Cite

show abstract

“…This RNA has an important secondary structure and is rather extensively "nicked" when extracted from particles harvested at long time intervals (3). It contains a poly(A) segment of large and homogeneous size (9). The virus particle, whose structure is very close to that of RNA tumor viruses (18,26), contains an RNA-dependent DNA polymerase (15,20,23) and replicates through the intermediate of a DNA provirus (11).…”

mentioning

confidence: 99%

Properties of Visna Virus Particles Harvested at Short Time Intervals: RNA Content, Infectivity, and Ultrastructure

Brahic

Vigne

1975

J Virol

View full text Add to dashboard Cite

and ethylenediacrylate were from Eastman Organic Chemicals.Acrylami'de and bisacrylamide were from Biorad.RNase-free sucrose, used for RNA work, was purchased from Schwarz-Mann.[3H ]uridine (20 Ci/mM) and carrier-free sP were from CEA, France. All other chemicals were obtained as reagent grade.TNE buffer refers to the following solution: 0.01 M Tris-hydrochloride (pH 7.5), 0.1 M NaCl, and 0.001 M EDTA. TNE-SDS buffer refers to the same solution containing 0.5% sodium dodecyl sulfate (SDS). For the RNA work, the buffers and plastic pieces of equipment were treated with diethylpyrocarbonate 1222 on July 7, 2020 by guest

show abstract

“…It has a buoyant density of 1.16 g/ml in sucrose (6)(7)(8), an RNAinstructed DNA polymerase (6,9,10), and a 60-70S RNA (6)(7)(8). This high-molecular-weight RNA contains poly-(adenylic acid) approximately 200 nucleotides in length (11)(12)(13) and consists of subunits of 2 to 3 X 106 daltons (G. Schochetman and J. Schlom, J. Virol., submitted for publication).…”

mentioning

confidence: 99%

“…Further studies were conducted to determine the degree of copying, if any, of poly(A) stretches known to be present in MPMV RNA (11)(12)(13). A cDNA containing poly(dT).stretches would be of limited value since this would result in hybridization to normal cellular messenger RNAs.…”

mentioning

confidence: 99%