Poly(ε-caprolactone) Nanofibers with a Self-Induced Nanohybrid Shish-Kebab Structure Mimicking Collagen Fibrils

Wang, Xiaofeng; Salick, Max R.; Wang, Xiaodong; Cordie, Travis; Han, Wenjuan; Peng, Yiyan; Li, Qian; Turng, Lih‐Sheng

doi:10.1021/bm400928b

Cited by 60 publications

(65 citation statements)

References 49 publications

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“…The first approach, controlled polymer crystallization on electrospun fibers either by incubation in polymer solution or by polymer solvent evaporation, creates lamellae on fibers with e.g. shish-kebab structures on fiber surfaces 5–7 . Alternatively, solvent extraction 8–10 , salt leaching 11 or calcination of electrospun fibers made up of two components yields porous surfaces by selective removal of the sacrificial component 12 .…”

Section: Introductionmentioning

confidence: 99%

Steering surface topographies of electrospun fibers: understanding the mechanisms

Yazgan

Dmitriev

Tyagi

et al. 2017

Sci Rep

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A profound understanding of how to tailor surface topographies of electrospun fibers is of great importance for surface sensitive applications including optical sensing, catalysis, drug delivery and tissue engineering. Hereby, a novel approach to comprehend the driving forces for fiber surface topography formation is introduced through inclusion of the dynamic solvent-polymer interaction during fiber formation. Thus, the interplay between polymer solubility as well as computed fiber jet surface temperature changes in function of time during solvent evaporation and the resultant phase separation behavior are studied. The correlation of experimental and theoretical results shows that the temperature difference between the polymer solution jet surface temperature and the dew point of the controlled electrospinning environment are the main influencing factors with respect to water condensation and thus phase separation leading to the final fiber surface topography. As polymer matrices with enhanced surface area are particularly appealing for sensing applications, we further functionalized our nanoporous fibrous membranes with a phosphorescent oxygen-sensitive dye. The hybrid membranes possess high brightness, stability in aqueous medium, linear response to oxygen and hence represent a promising scaffold for cell growth, contactless monitoring of oxygen and live fluorescence imaging in 3-D cell models.

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Section: Introductionmentioning

confidence: 99%

Steering surface topographies of electrospun fibers: understanding the mechanisms

Yazgan

Dmitriev

Tyagi

et al. 2017

Sci Rep

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“…Therefore, it is of interest to study the influence of this surface modification on cell growth and attachment to the NFSK surface. Studies in literature have proven that PCL nanofiber mats and NFSKs can support cell growth . Wang et al recently showed that cells were able to survive when seeded directly onto PCL NFSK scaffolds .…”

Section: Cytocompatibility Of Nfsksmentioning

confidence: 99%

“…50 Wang et al recently showed that cells were able to survive when seeded directly onto PCL NFSK scaffolds. 50 In the current work, in addition to unadorned PCL nanofibers, unmineralized NFSKs and NFSKs after 2 and 5 days of mineralization were evaluated for cytotoxicity. A live/dead assay was used to examine the cytocompatibility of cells attached to the nanofiber mat surface.…”

Section: Cytocompatibility Of Nfsksmentioning

confidence: 99%

Hierarchically ordered polymer nanofiber shish kebabs as a bone scaffold material

Chen

Gleeson

et al. 2017

J Biomedical Materials Res

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The ideal bone scaffold harnesses the body's ability to regenerate bone in critical size defects. A potential strategy to design the ideal bone scaffold is to mimic the natural structure of bone at the molecular level. The orientation and spatial distribution of nanocrystals in the organic matrix are two important and distinctive structural characteristics associated with natural bone. There has yet to be a synthetic system or scaffold that is able to control both the spatial distribution and orientation of the hydroxyapatite crystalline structure. To mimic the unique hybrid structure of natural bone using synthetic materials, there have been a number of reported approaches to control the mineral nanocrystal orientation on synthetic scaffolds. However, the spatial distribution of minerals is challenging to reproduce in a biomimetic manner. Herein we report using block copolymer-decorated polymer nanofibers to achieve biomineralized fibrils with precise control of both mineral crystal orientation and spatial distribution. We show that the crystallization of poly(caprolactone)-b-poly(acrylic acid) block copolymer on poly(caprolactone) nanofibers resulted in a unique shish kebab structure that significantly enhances the mechanical properties of the nanofibers, and is cytocompatible to L-929 mouse fibroblast cells. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1786-1798, 2017.

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“…A strategy that is currently being explored is to control for the mineral organization including spatial distribution and orientation during the mineralization process, which can be achieved using a template called nanofiber shish kebabs (NFSK) . NFSK are formed via polymer crystallization of electrospun nanofibers as shown in Figure . Our group has previously described this method to mimic mineralized collagen fibrils of bone .…”

Section: Introductionmentioning

confidence: 99%

Electrospun poly(ε‐caprolactone) nanofiber shish kebabs mimic mineralized bony surface features

Gleeson

Marcolongo

2018

J Biomed Mater Res

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Electrospinning of nanofiber is of growing interest especially in bone tissue engineering because of its similar fibrous properties to the extracellular matrix. To this end, we have fabricated polycaprolactone (PCL) nanofiber shish kebab (NFSK) templates. The novelty of this work is the ability to control the mineral orientation and spatial location on the nanofiber, mimicking natural collagen fibers. However, NFSK templates have properties that need to be investigated in terms of cellular response including fiber alignment and crystallization. In this study, MC3T3 E1 preosteoblast cells were seeded onto the templates to determine the effect of both fiber orientation and kebab size on the cell metabolic activity. PCL was electrospun to form aligned and randomly oriented nanofibers, which were then crystallized in a PCL solution in pentyl acetate for 15 and 60 min, resulting in the formation of homopolymer PCL NFSK templates. We evaluated the cell proliferation and alkaline phosphatase activity of MC3T3 E1 cells after 3, 7, and 14 days in coculture. Aligned nanofiber and polymer crystallization both significantly increased the cell proliferation and alkaline phosphatase activity at each time point. The aligned nanofibers and polymer crystallization resulted in the highest metabolic activities of the cells compared to the randomly oriented fibers and noncrystallized controls.

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Poly(ε-caprolactone) Nanofibers with a Self-Induced Nanohybrid Shish-Kebab Structure Mimicking Collagen Fibrils

Cited by 60 publications

References 49 publications

Steering surface topographies of electrospun fibers: understanding the mechanisms

Steering surface topographies of electrospun fibers: understanding the mechanisms

Hierarchically ordered polymer nanofiber shish kebabs as a bone scaffold material

Electrospun poly(ε‐caprolactone) nanofiber shish kebabs mimic mineralized bony surface features

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