Poly (ε-caprolactone)/layered double hydroxide microspheres-aggregated nanocomposite scaffold for osteogenic differentiation of mesenchymal stem cell

baradaran, Tina; Shafiei, Seyedeh Sara; Mohammadi, Sepideh; Moztarzadeh, Fathollah

doi:10.1016/j.mtcomm.2020.100913

Cited by 20 publications

(20 citation statements)

References 36 publications

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“…Synthetic [i.e., poly(ε‐caprolactone)] and natural polymers (i.e., chitosan, collagen, alginate, agarose, hyaluronic acid, and fibrin) have been largely proposed to manufacture scaffolds for hard and soft tissue regeneration. [ 4–7 ]…”

Section: Introductionmentioning

confidence: 99%

Photo‐Curing 3D Printing and Innovative Design of Porous Composite Structures for Biomedical Applications

Santis

Russo

Fucile

et al. 2021

Macromolecular Symposia

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Light‐activated resins and composites are used in conjunction with a light curing unit and allow an on‐demand process of polymerization. These kinds of materials usually represent the most popular choice in the restorative dental practice. Some works have already highlighted contemporary tendencies in the use of nondegradable scaffolds and mesenchymal stem cells in regenerative medicine. Accordingly, the aim of the current research is to develop 3D porous and light‐activated composite structures with optimized functional properties. Preliminary mechanical and biological tests are carried out.

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Section: Introductionmentioning

confidence: 99%

Photo‐Curing 3D Printing and Innovative Design of Porous Composite Structures for Biomedical Applications

Santis

Russo

Fucile

et al. 2021

Macromolecular Symposia

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“…A recent study also focused on the development of PCL/layered double hydroxide (LDH) microsphere-aggregated nanocomposite scaffolds as suitable candidates for bone tissue engineering [11]. The results demonstrated that the inclusion of LDH nanoparticles improved the osteogenic differentiation and mechanical properties (e.g., compressive modulus) of mesenchymal stem cells.…”

Section: Introductionmentioning

confidence: 99%

“…The results demonstrated that the inclusion of LDH nanoparticles improved the osteogenic differentiation and mechanical properties (e.g., compressive modulus) of mesenchymal stem cells. depending upon the LDH amount [11].…”

Section: Introductionmentioning

confidence: 99%

Combination Design of Time-Dependent Magnetic Field and Magnetic Nanocomposites to Guide Cell Behavior

et al. 2020

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The concept of magnetic guidance is still challenging and has opened a wide range of perspectives in the field of tissue engineering. In this context, magnetic nanocomposites consisting of a poly(ε-caprolactone) (PCL) matrix and iron oxide (Fe3O4) nanoparticles were designed and manufactured for bone tissue engineering. The mechanical properties of PCL/Fe3O4 (80/20 w/w) nanocomposites were first assessed through small punch tests. The inclusion of Fe3O4 nanoparticles improved the punching properties as the values of peak load were higher than those obtained for the neat PCL without significantly affecting the work to failure. The effect of a time-dependent magnetic field on the adhesion, proliferation, and differentiation of human mesenchymal stem cells (hMSCs) was analyzed. The Alamar Blue assay, confocal laser scanning microscopy, and image analysis (i.e., shape factor) provided information on cell adhesion and viability over time, whereas the normalized alkaline phosphatase activity (ALP/DNA) demonstrated that the combination of a time-dependent field with magnetic nanocomposites (PCL/Fe3O4 Mag) influenced cell differentiation. Furthermore, in terms of extracellular signal-regulated kinase (ERK)1/2 phosphorylation, an insight into the role of the magnetic stimulation was reported, also demonstrating a strong effect due the combination of the magnetic field with PCL/Fe3O4 nanocomposites (PCL/Fe3O4 Mag).

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“…In recent years, to obtain suitable orthopedic biomaterials to allow the full healing of bone defects before their complete degradation, studies have been carried out to combine magnesium-based materials with diverse biodegradable and biocompatible three dimensional (3D) porous scaffolds [1,[4][5][6][12][13][14][15][16][17][18][19][20][21][22]. Scaffolds are supporting structural devices that can influence the behavior of cells in bone tissue regeneration processes [17,[23][24][25].…”

Section: Introductionmentioning

confidence: 99%

“…It is also hydrophobic, adversely influencing cell adhesion and proliferation events [25,26,28,29]. For these reasons, specific investigations combining properties of PCL scaffolds with those of magnesium-based materials may represent a new avenue for bone tissue engineering [16][17][18][19][20]. In recent years, surface modification techniques have gained great importance for their ability to improve interactions of PCL with osteoconductive cells for the purpose of bone tissue regeneration [20,25,26].…”

Section: Introductionmentioning

confidence: 99%

Plasma-Assisted Deposition of Magnesium-Containing Coatings on Porous Scaffolds for Bone Tissue Engineering

et al. 2020

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Magnesium plays a pivotal role in the formation, growth, and repair of bone tissue; therefore, magnesium-based materials can be considered promising candidates for bone tissue engineering. This study aims to functionalize the surfaces of three-dimensional (3D) porous poly-ε caprolactone (PCL) scaffolds with magnesium-containing coatings using cold plasma-assisted deposition processes. For this purpose, the radiofrequency (RF) sputtering of a magnesium oxide target was carried out in a low-pressure plasma reactor using argon, water vapor, hydrogen, or mixtures of argon with one of the latter two options as the feed. Plasma processes produced significant differences in the chemical composition and wettability of the treated PCL samples, which are tightly related to the gas feed composition, as shown by X-ray photoelectron spectroscopy (XPS) and water contact angle (WCA) analyses. Cytocompatibility assays performed with Saos-2 osteoblast cells showed that deposited magnesium-containing thin films favor cell proliferation and adhesion on 3D scaffold surfaces, as well as cell colonization inside them. These films appear to be very promising for bone tissue regeneration.

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Poly (ε-caprolactone)/layered double hydroxide microspheres-aggregated nanocomposite scaffold for osteogenic differentiation of mesenchymal stem cell

Cited by 20 publications

References 36 publications

Photo‐Curing 3D Printing and Innovative Design of Porous Composite Structures for Biomedical Applications

Photo‐Curing 3D Printing and Innovative Design of Porous Composite Structures for Biomedical Applications

Combination Design of Time-Dependent Magnetic Field and Magnetic Nanocomposites to Guide Cell Behavior

Plasma-Assisted Deposition of Magnesium-Containing Coatings on Porous Scaffolds for Bone Tissue Engineering

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