2012
DOI: 10.1021/la205162d
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Poly(ε-caprolactone)-Banded Spherulites and Interaction with MC3T3-E1 Cells

Abstract: We report that protein adsorption, cell attachment, and cell proliferation were enhanced on spherulites-roughened polymer surfaces. Banded spherulites with concentric alternating succession of ridges and valleys were observed on spin-coated thin films of poly(ε-caprolactone) (PCL) and two series of PCL binary homoblends composed of high- and low-molecular-weight components when they were isothermally crystallized at 25-52 °C. Their thermal properties, crystallization kinetics, and surface morphology were exami… Show more

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Cited by 32 publications
(22 citation statements)
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“…The present results can be compared with our recent reports on two groups of substrates with concentric microgrooves 13, 40. Consistently, photo‐crosslinked PCLTA substrates with highly uniform 7.5‐μm‐wide and 10‐μm‐deep concentric grooves enhanced MC3T3‐E1 cell alignment and mineralization; however, different microgroove dimensions did not influence cell attachment and proliferation in that study and no groove width smaller than 7.5 μm was used 13.…”
Section: Resultssupporting
confidence: 88%
See 1 more Smart Citation
“…The present results can be compared with our recent reports on two groups of substrates with concentric microgrooves 13, 40. Consistently, photo‐crosslinked PCLTA substrates with highly uniform 7.5‐μm‐wide and 10‐μm‐deep concentric grooves enhanced MC3T3‐E1 cell alignment and mineralization; however, different microgroove dimensions did not influence cell attachment and proliferation in that study and no groove width smaller than 7.5 μm was used 13.…”
Section: Resultssupporting
confidence: 88%
“…Cell alignment was less significant when the aspect ratio of grooves, defined by dividing the groove depth by the width, was smaller, because cells cannot sense the shallow surface topography efficiently 46. For MC3T3‐E1 cells seeded on poly(ϵ‐caprolactone) banded spherulites with the groove depth of ∼350 nm and the groove width of ∼30 μm, cell attachment and proliferation were better than those on flat hot‐compressed disks but no cell alignment was observed because of the low aspect ratio of grooves 40. In another report, focal conic structures with 700‐nm‐high ridges spaced ∼8.5 μm apart were found on the solvent cast films of cholesteryl oligo(L‐lactic acid) and 3T3 fibroblasts could survive and proliferate better on these self‐assembled domains and spread preferentially along the domain boundaries but not along the concentric rings 47.…”
Section: Resultsmentioning
confidence: 99%
“…Since the 3D macroporous MWCNT and SWCNT scaffolds could be utilized as implants for bone tissue engineering applications, they will primarily interact with precursor osteoblasts cells in vivo . Thus, MC3T3 cells, a widely accepted cell line for in vitro bone studies were utilized as model cell lines to evaluate the in vitro cytocompatibility of MWCNT and SWCNT scaffolds [56]. …”
Section: Discussionmentioning
confidence: 99%
“…Thus, MC3T3 cells, a widely accepted cell line for in vitro bone studies were utilized as model cell lines to evaluate the in vitro cytocompatibility of MWCNT and SWCNT scaffolds. 56 To quantitatively assess the cytotoxicity of MWCNT and SWCNT scaffolds, we performed LDH assay to assess cell death of MC3T3 cells after 1, 3, and 5 days of culture. Porous PLGA scaffolds were used as live controls.…”
Section: Discussionmentioning
confidence: 99%
“…The loading concentration of each nanomaterial was the concentration that showed maximum reinforcement of PPF polymer in our previous studies [16, 17]. The adherent NIH3T3 fibroblast-like cells [46, 47] and MC3T3 osteoblast precursor cells [48, 49] used in this study are widely accepted for in vitro cytotoxicity testing of materials of orthopedic or bone tissue engineering applications. In vitro cytotoxicity studies were performed before crosslinking, after crosslinking, and after accelerated degradation of the nanocomposites.…”
Section: Discussionmentioning
confidence: 99%