Biomimetic Calcium Carbonate Concentric Microgrooves with Tunable Widths for Promoting MC3T3‐E1 Cell Functions

Wu, Xiaohui; Wang, Shanfeng

doi:10.1002/adhm.201200205

Cited by 32 publications

(27 citation statements)

References 49 publications

(70 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[30][31][32] Many studies have investigated the interaction of osteoblasts with porous Ta, Ta discs, and Ta coatings, but limited research has been performed on the interaction of osteoblasts with Ta-NPs. Ninomiya et al 14 compared the effects of various implant surfaces (Ti mesh, cobalt-chromium beads, and porous Ta) on osteoblast proliferation and found that the osteoblast proliferation on porous Ta was significantly greater than that on the other surfaces, thus revealing porous Ta as a superior scaffold.…”

Section: Discussionmentioning

confidence: 99%

Involvement of autophagy in tantalum nanoparticle-induced osteoblast proliferation

Kang¹,

Wei²,

Song³

et al. 2017

IJN

View full text Add to dashboard Cite

Porous tantalum (Ta) implants are highly corrosion resistant and biocompatible, and they possess significantly better initial stability than that of conventional titanium (Ti) implants. During loading wear, Ta nanoparticles (Ta-NPs) that were deposited on the surface of a porous Ta implant are inevitably released and come into direct contact with peri-implant osteoblasts. The wear debris may influence cell behavior and implant stabilization. However, the interaction of Ta-NPs with osteoblasts has not been clearly investigated. This study aimed to investigate the effect of Ta-NPs on cell proliferation and their underlying mechanism. The Cell Counting Kit-8 (CCK-8) assay was used to measure the cell viability of MC3T3-E1 mouse osteoblasts and showed that Ta-NP treatment could increase cell viability. Then, confocal microscopy, Western blotting, and transmission electron microscopy were used to confirm the autophagy induced by Ta-NPs, and evidence of autophagy induction was observed as positive LC3 puncta, high-LC3-II expression, and autophagic vesicle ultrastructures. The CCK-8 assay revealed that the cell viability was further increased and decreased by the application of an autophagy inducer and inhibitor, respectively. In addition, pre-treatment with autophagy inhibitor 3-methyladenine (3-MA) inhibited the Ta-NP-induced autophagy. These results indicate that the Ta-NPs can promote cell proliferation, that an autophagy inducer can further strengthen this effect and that an autophagy inhibitor can weaken this effect. In conclusion, autophagy was involved in Ta-NP-induced cell proliferation and had a promoting effect.

show abstract

Section: Discussionmentioning

confidence: 99%

Involvement of autophagy in tantalum nanoparticle-induced osteoblast proliferation

Kang¹,

Wei²,

Song³

et al. 2017

IJN

View full text Add to dashboard Cite

show abstract

“…[34][35][36][37][38][39] In this study, the ALP activity of MG63 cells on the VH/rhBMP-2/n-MZS exhibited significantly higher levels of expression than that on the VH/rhBMP-2/MZS system at 7 days, indicating that the cells differentiated more quickly …”

Section: Alp Activitymentioning

confidence: 55%

Sustained release of VH and rhBMP-2 from nanoporous magnesium–zinc–silicon xerogels for osteomyelitis treatment and bone repair

Li¹,

Wu²,

Li³

et al. 2015

IJN

View full text Add to dashboard Cite

Nanoporous magnesium–zinc–silicon (n-MZS) xerogels with a pore size ∼4 nm, a surface area of 718 cm 2 /g, and a pore volume of 1.24 cm 3 /g were synthesized by a sol–gel method. The n-MZS xerogels had high capacity to load vancomycin hydrochloride (VH) and human bone morphogenetic protein-2 (rhBMP-2), after soaking in phosphate buffered saline (PBS) for 24 hours (1.5 and 0.8 mg/g, respectively). Moreover, the n-MZS xerogels exhibited the sustained release of VH and rhBMP-2 as compared with magnesium–zinc–silicon (MZS) xerogels without nanopores (showing a burst release). The VH/rhBMP-2/n-MZS system not only exhibited a good antibacterial property but also promoted the MG63 cell proliferation and differentiation demonstrating good bactericidal activity and cytocompatibility. The results suggested that n-MZS with larger surface area and high pore volume might be a promising carrier for loading and sustained release of VH and rhBMP-2. Hence, the VH/rhBMP-2/n-MZS system might be one of the promising biomaterials for osteomyelitis treatment and bone repair.

show abstract

“…30 The transition from newly formed bone to a mature one is a gradual process where there is a dynamic interaction between scaffolds and the invasion of bone cells/tissues. [31][32][33] In this study, it was found that new bone tissues gradually increased and the materials continued to reduce for both l-MBPC and l-BPC scaffolds after implanting at different time points. At 3 months, a large quantity of new bone tissues formed in l-MBPC scaffolds, which was significantly higher than in l-BPC scaffolds.…”

mentioning

confidence: 59%

Degradability, bioactivity, and osteogenesis of biocomposite scaffolds of lithium-containing mesoporous bioglass and mPEG-PLGA-b-PLL copolymer

Cai¹,

Guo²,

Shen³

et al. 2015

IJN

View full text Add to dashboard Cite

Biocomposite scaffolds of lithium (Li)-containing mesoporous bioglass and monomethoxy poly(ethylene glycol)-poly(D,L-lactide- co -glycolide)-poly(L-lysine) (mPEG-PLGA- b -PLL) copolymer were fabricated in this study. The results showed that the water absorption and degradability of Li-containing mesoporous bioglass/mPEG-PLGA- b -PLL composite (l-MBPC) scaffolds were obviously higher than Li-containing bioglass/mPEG-PLGA -b -PLL composite (l-BPC) scaffolds. Moreover, the apatite-formation ability of l-MBPC scaffolds was markedly enhanced as compared with l-BPC scaffolds, indicating that l-MBPC scaffolds containing mesoporous bioglass exhibited good bioactivity. The cell experimental results showed that cell attachment, proliferation, and alkaline phosphatase activity of MC3T3-E1 cells on l-MBPC scaffolds were remarkably improved as compared to l-BPC scaffolds. In animal experiments, the histological elevation results revealed that l-MBPC scaffolds significantly promoted new bone formation, indicating good osteogenesis. l-MBPC scaffolds with improved properties would be an excellent candidate for bone tissue repair.

show abstract

Biomimetic Calcium Carbonate Concentric Microgrooves with Tunable Widths for Promoting MC3T3‐E1 Cell Functions

Cited by 32 publications

References 49 publications

Involvement of autophagy in tantalum nanoparticle-induced osteoblast proliferation

Involvement of autophagy in tantalum nanoparticle-induced osteoblast proliferation

Sustained release of VH and rhBMP-2 from nanoporous magnesium–zinc–silicon xerogels for osteomyelitis treatment and bone repair

Degradability, bioactivity, and osteogenesis of biocomposite scaffolds of lithium-containing mesoporous bioglass and mPEG-PLGA-b-PLL copolymer

Contact Info

Product

Resources

About

Biomimetic Calcium Carbonate Concentric Microgrooves with Tunable Widths for Promoting MC3T3‐E1 Cell Functions

Cited by 32 publications

References 49 publications

Involvement of autophagy in tantalum nanoparticle-induced osteoblast proliferation

Involvement of autophagy in tantalum nanoparticle-induced osteoblast proliferation

Sustained release of VH and rhBMP-2 from nanoporous magnesium&ndash;zinc&ndash;silicon xerogels for osteomyelitis treatment and bone repair

Degradability, bioactivity, and osteogenesis of biocomposite scaffolds of lithium-containing mesoporous bioglass and mPEG-PLGA-b-PLL copolymer

Contact Info

Product

Resources

About

Sustained release of VH and rhBMP-2 from nanoporous magnesium–zinc–silicon xerogels for osteomyelitis treatment and bone repair