Poly-pharmacokinetic strategy-delineated metabolic fate of bioactive compounds in a traditional Chinese medicine formula, Yuanhu Zhitong tablets, using parallel reaction monitoring mode

Wang, Ping; Zhang, Tinglan; Yang, Guohua; Li, Mengjie; Zhang, Jiaqi; Li, Ke; Fu, Yan; Xu, Haiyu; Yang, Hongjun

doi:10.1016/j.phymed.2018.09.026

Cited by 16 publications

(5 citation statements)

References 9 publications

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“…e selection of IS is also a very important part for the stability and accuracy of quantitative results. It is reported [23] that promethazine hydrochloride showed appropriate chromatographic behaviors and good response in positive mode when it as IS for the in vivo quantitative analyses of YHZT preparations.…”

Section: Uplc-ms/ms Condition Optimizationmentioning

confidence: 99%

Simultaneous Determination of Six Components in Beagle Dog Plasma by UPLC-MS/MS and Its Application to a Comparative Pharmacokinetic Study of Three Different Yuanhu Zhitong Preparations

Wang

Yin

Zhang

et al. 2022

Journal of Chemistry

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This study has developed a sensitive, simple, and reliable ultrahigh performance liquid chromatography tandem mass spectrometry method for the simultaneous determination of corydaline, tetrahydropalmatine, tetrahydroberberine, tetrahydrocoptisine, byakangelicin, and byakangelicol in beagle dog plasma after oral administration of Yuanhu Zhitong (YHZT) oral liquid, dispersible tablet, and dropping pills. A one-step protein precipitation method was used with methanol-acetonitrile (50 : 50, v/v) for plasma sample pretreatment. Six analytes and one internal standard were separated on a Waters Acquity UPLC BEH C18 column (2.1 mm × 100 mm, 1.7 μm) by gradient elution with 0.1% formic acid-water and 0.1% formic acid-acetonitrile at a flow rate of 0.4 mL/min. Detection was conducted by multiple reaction monitoring mode in the positive ionization. The calibration curves showed good linearity (r > 0.9920). The lower limit of quantization ranged from 0.1 to 1 ng/mL. Moreover, the other results of the method validation were within the acceptable range. After oral administration, the pharmacokinetic characteristics of the three YHZT preparations were different. Compared with the two solid preparations, the absorption of the six analytes in oral liquid was more rapid. Moreover, the area under the plasma drug concentration-time curve and the maximum plasma concentration of tetrahydropalmatine (the main analgesic component of YHZT prescription) in the oral liquid group were higher than those in the other two groups. The results showed oral liquid can exert its efficacy quickly, while dispersible tablet and dropping pills exhibit relatively slow release pattern. The comparative pharmacokinetic study would be helpful to the clinical rational selection and application of YHZT preparations.

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Section: Uplc-ms/ms Condition Optimizationmentioning

confidence: 99%

Simultaneous Determination of Six Components in Beagle Dog Plasma by UPLC-MS/MS and Its Application to a Comparative Pharmacokinetic Study of Three Different Yuanhu Zhitong Preparations

Wang

Yin

Zhang

et al. 2022

Journal of Chemistry

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“…Findings revealed the relationships between multiple compounds of Huangqi decoction, their corresponding metabolites, and their effects on metabolism, providing unprecedented understanding of the mechanisms of action of Huangqi decoction. Very recently, Wang, Li, Tao, et al (2019) performed pharmacokinetic evaluation of rats after oral administration of eight constituents in Yuanhu Zhitong tablets (Ping et al, 2019) or Yuanhu Zhitong prescription (i.e., a commonly used clinical herb preparation recorded in the China Pharmacopoeia for the treatment of stomach pain, hypochondriac pain, headache, and dysmenorrhea), Corydalis yanhusuo, and Angelica dahurica (Hoffm. ).…”

Section: Application Of Polypharmacokinetic Strategy To Mfhmentioning

confidence: 99%

“…Very recently, Wang, Li, Tao, et al. (2019) performed pharmacokinetic evaluation of rats after oral administration of eight constituents in Yuanhu Zhitong tablets (Ping et al., 2019) or Yuanhu Zhitong prescription (i.e., a commonly used clinical herb preparation recorded in the China Pharmacopoeia for the treatment of stomach pain, hypochondriac pain, headache, and dysmenorrhea), Corydalis yanhusuo, and Angelica dahurica (Hoffm. ).…”

Section: Perspectives For the Futurementioning

confidence: 99%

Advances in understanding of health‐promoting benefits of medicine and food homology using analysis of gut microbiota and metabolomics

et al. 2020

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The health-promoting benefits of medicine and food homology (MFH) are known for thousands of years in China. However, active compounds and biological mechanisms are unclear, greatly limiting clinical practice of MFH. The advent of gut microbiota analysis and metabolomics emerge as key tools to discover functional compounds, therapeutic targets, and mechanisms of benefits of MFH. Such studies hold great promise to promote and optimize functional efficacy and development of MFH-based products, for example, foods for daily dietary supplements or for special medical purposes. In this review, we summarized pharmacological effects of 109 species of MFH approved by the Health and Fitness Commission in 2015. Recent studies applying genome sequencing of gut microbiota and metabolomics to explain the activity of MFH in prevention and management of health consequences were extensively reviewed. We discussed the potentiality in future to decipher functional activities of MFH by applying metabolomics-based polypharmacokinetic strategy and multiomics technologies. The needs for personalized MFH recommendations and comprehensive databases have also been highlighted. This review emphasizes current achievements and challenges of the analysis of gut microbiota and metabolomics as a new avenue to understand MFH. K E Y W O R D S gut microbiota, medicine and food homology, metabolomics, multiomics technology Abbreviations: IBD, inflammatory bowel disease; LPS, lipopolysaccharide; MFH, medicine and food homology; SCFA, short-chain fatty acid; TCMIO, a comprehensive database of traditional Chinese medicine on immuno-oncology; WEGL, water extract of Ganoderma lucidum mycelium This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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“…In this work, we employed the Thermo-Fisher Dionex UltiMate 3000 UHPLC-Q Exactive Orbitrap-MS system, with a high-resolution and highthroughput platform to conduct the PK study of 38 marker compounds (due to the low concentration of the other 17 components, the time points that could be detected were less than six, their PK curves were not available), and the mass spectrum information and extracted ion ranges of the 38 components were listed in Table 1. The method was based on a high-resolution full-scan mode, to acquire a comprehensive profile of all ionized components in rat plasma, and previous studies have confirmed the reliability of this analytical method in PK studies (Wang et al, 2019). So, based on the above research, the impact on pharmacokinetic parameters before and after the combination of RB and RPA was analyzed by multi-component pharmacokinetics research method.…”

Section: Introductionmentioning

confidence: 99%

Studies on the Changes of Pharmacokinetics Behaviors of Phytochemicals and the Influence on Endogenous Metabolites After the Combination of Radix Bupleuri and Radix Paeoniae Alba Based on Multi-Component Pharmacokinetics and Metabolomics

et al. 2021

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Radix Bupleuri-Radix Paeoniae Alba (RB-RPA) is a classic herb pair, which is commonly used to treat depression by soothing “liver qi stagnation” in the clinic. However, little is yet known concerning the combination mechanism of Radix Bupleuri (RB) and Radix Paeoniae Alba (RPA), their bioactive forms in vivo and the regulatory effects on the organism. The present study aimed to elucidate the changes in multi-component pharmacokinetics (PK) behavior after the combination of RB and RPA by a high-resolution full-scan mode of UPLC-HRMS method (a total of 38 components PK profiles were obtained, of which 23 components come from RB and 15 components come from RPA). Moreover, the metabolomics approach was used to analyze the dynamic response of endogenous metabolites intervened by RB-RPA, and the correlation between concentration-time curves of 38 components from RB-RPA and the dynamic response profiles of endogenous metabolites was characterized by Pearson correlation analysis. The results demonstrated that the combination of RB and RPA could significantly improve the bioavailability of five components in RB, and six components in RPA. Besides, metabolomics results indicated that a total of 21 endogenous metabolites exhibited time-dependent changes in response to the RB-RPA administration, of which 12 endogenous metabolites were significantly increased, and nine endogenous metabolites were significantly decreased. Furthermore, correlation analysis results indicated that the components with significantly improved bioavailability after combination such as saikogenin F, saikogenin G, albiflorin, methyl gallate, paeonimetabolin II were significantly positively correlated with picolinic acid, a metabolite with neuroprotective effect; saikogenin F, saikogenin G were significantly positively correlated with itaconic acid, a endogenous metabolite with anti-inflammatory activity; and albiflorin, paeonimetabolin II were significantly positively correlated with α-linolenic acid, a metabolite with strong protective actions on brain functions. These results indicated that the combination of RB and RPA can enhance each other’s neuroprotective and anti-inflammatory activities. In this study, A novel and efficient strategy has been developed to analyze the influence of the combination of RB and RPA in vivo behaviors by combining multi-component pharmacokinetics with metabolomics, which was contributed to clarifying the scientific connotation of herb–herb compatibility.

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Poly-pharmacokinetic strategy-delineated metabolic fate of bioactive compounds in a traditional Chinese medicine formula, Yuanhu Zhitong tablets, using parallel reaction monitoring mode

Cited by 16 publications

References 9 publications

Simultaneous Determination of Six Components in Beagle Dog Plasma by UPLC-MS/MS and Its Application to a Comparative Pharmacokinetic Study of Three Different Yuanhu Zhitong Preparations

Simultaneous Determination of Six Components in Beagle Dog Plasma by UPLC-MS/MS and Its Application to a Comparative Pharmacokinetic Study of Three Different Yuanhu Zhitong Preparations

Advances in understanding of health‐promoting benefits of medicine and food homology using analysis of gut microbiota and metabolomics

Studies on the Changes of Pharmacokinetics Behaviors of Phytochemicals and the Influence on Endogenous Metabolites After the Combination of Radix Bupleuri and Radix Paeoniae Alba Based on Multi-Component Pharmacokinetics and Metabolomics

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